User:Dyd5192

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	BACAN:LEF	2015-10-14 11:47:27 CDT	GO:0008237 metallopeptidase activity (F)	PMID:7854123	ECO:0000247 sequence alignment evidence used in manual assertion	Figure 1. Shows DNA sequence comparison of LEF with other proteins from different organisms that present metallopeptidase activity. Lethal factor sequence contain the HEXXH motif inferring its metallopeptidase activity	challenge
unacceptable	BACAN:LEF	2015-10-14 11:53:16 CDT	GO:0008270 zinc ion binding (F)	PMID:7854123	ECO:0000314 direct assay evidence used in manual assertion	Figure 3. Shows LEF binding to radioactive labeled zinc binding	challenge
unacceptable	STAAU:Q7WUJ5	2015-11-11 11:22:56 CST	GO:0090484 drug transporter activity (F)	PMID:26430941	ECO:0000314 direct assay evidence used in manual assertion	Figure 2. Ethidium Bromide accumulation assay shows that qacB overexpression results in efflux pumps that result in methicillin-resistant Staphylococcus aureus (MRSA)	challenge
acceptable	CLOTE:TETX	2015-11-17 10:37:19 CST	GO:0004222 metalloendopeptidase activity (F)	PMID:1396558	ECO:0000314 direct assay evidence used in manual assertion	Figure 7. Shows that the L chain of TeTx when bound to zinc is able to proteolytically cleave Synaptobrevin	challenge
unacceptable	CLOBO:BXD	2015-11-17 11:07:07 CST	GO:0044164 host cell cytosol (C)	PMID:15584922	ECO:0000314 direct assay evidence used in manual assertion	Figure 6a. Shows that Baf A1pre-treatment drastically decreased cleavage of synaptobrevin 2 by BoNT/D and GFP-BoNT/D evidencing that both proteins pass through acidic compartments en route delivering the catalytic domain to the cytosol. Figure 6b. Shows that GFP-BoNT/D co-localizes with transferrin	challenge
acceptable	SERME:PRZN	2015-11-17 11:15:04 CST	GO:0006508 proteolysis (P)	PMID:26453184	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1b. The proteolytic activity of recombinant wild type Spep was confirmed by zymogram analysis	challenge
unacceptable	STAEP:GSEA	2015-11-17 12:06:05 CST	GO:0008233 peptidase activity (F)	PMID:23970550	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1C. Esp activity assay was performed and enzymatic activity was measured. Figure 1D. purified pro-Esp, Esp, or EspS235A was incubated with S. aureus Newman during assembly of biofilms and graph shoes considerable reduction on biofilm formation for Esp sample. In conclusion, purified, recombinant Esp displays protease activity, inhibiting S. aureus biofilm formation and cleaving Atl	challenge
unacceptable	STAEP:GSEA	2015-11-17 12:23:11 CST	GO:0009405 pathogenesis (P)	PMID:23970550	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 6. Shows that Esp blocks the release of extracellular DNA during biofilm formation	challenge
unacceptable	STAAU:B0YLX3	2015-11-20 09:12:26 CST	GO:0009405 pathogenesis (P)	PMID:24847020	ECO:0000314 direct assay evidence used in manual assertion	Figure 2A and 2B. Images show macrophages and neutrophils being killed by PVL. Characteristic membrane halos and expansion of cellular nuclei are seen, along with increased permeability to ethidium bromide (indicator of pore formation and membrane damage)	challenge
acceptable	STAAU:B0YLX3	2015-11-20 09:18:48 CST	GO:0009405 pathogenesis (P)	PMID:26091719	ECO:0000314 direct assay evidence used in manual assertion	Figure 1A. Pore formation in freshly isolated neutrophils from different mammalian species after incubation with PVL shows differential species-specific cytotoxicity of PVL. PVL is active only toward human and rabbit neutrophils, but not toward neutrophils from macaque, cow, and mouse	challenge

acceptable:4
unacceptable:6
requires_changes:0
flagged:0

Annotations challenged by Dyd5192

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	Dyd5192, Team PSU 1	BACAN:LEF	GO:0008237 - metallopeptidase activity (F)	PMID:7854123	ECO:0000247 sequence alignment evidence used in manual assertion	challenge	C: 4
updatedbyinstructor	Yeyuan, Team Bob Ross	HUMAN:ITCH	GO:0006511 - ubiquitin-dependent protein catabolic process (P)	PMID:25518932	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 3 fixed by Dyd5192
updatedbyinstructor	Jkane94, Team MIA	HUMAN:TPX2	GO:0007026 - negative regulation of microtubule depolymerization (P)	PMID:26414402	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1 fixed by Dyd5192
updatedbyinstructor	Kaylamichbrown, Team MIA	YEAST:SIR2	GO:0031047 - regulatory ncRNA-mediated gene silencing (P)	PMID:9009207	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2
unacceptable	Zacharymax, Team G-nomes	HUMAN:MAVS	GO:0060340 - positive regulation of type I interferon-mediated signaling pathway (P)	PMID:25636800	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
unacceptable	Zacharymax, Team G-nomes	HUMAN:MAVS	GO:0060340 - positive regulation of type I interferon-mediated signaling pathway (P)	PMID:25636800	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1
unacceptable	Zacharymax, Team G-nomes	HUMAN:TCAM1	GO:0004674 - protein serine/threonine kinase activity (F)	PMID:25636800	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1
updatedbyinstructor	Zacharymax, Team G-nomes	HUMAN:TCAM1	GO:0035666 - TRIF-dependent toll-like receptor signaling pathway (P)	PMID:25636800	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1 fixed by Dyd5192
updatedbyinstructor	Zacharymax, Team G-nomes	HUMAN:UBP15	GO:1990380 - K48-linked deubiquitinase activity (F)	PMID:24399297	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2
updatedbyinstructor	Vvg0009, Team Hardy Weinburgers	ARATH:SCL23	GO:0001714 - endodermal cell fate specification (P)	PMID:26415082	ECO:0000316 genetic interaction evidence used in manual assertion	challenge	C: 2
updatedbyinstructor	Annie luca, Team You cannot B. cereus	BRUA2:Q2YN91	GO:0052170 - symbiont-mediated suppression of host innate immune response (P)	PMID:23847770	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
updatedbyinstructor	Sandyl 27, Team Sandy Konas	BACSU:FLIW	GO:0071978 - bacterial-type flagellum-dependent swarming motility (P)	PMID:21895793	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1 fixed by Dyd5192
unacceptable	Zacharymax, Team G-nomes	HUMAN:TBK1	GO:0060340 - positive regulation of type I interferon-mediated signaling pathway (P)	PMID:25636800	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
unacceptable	Zacharymax, Team G-nomes	HUMAN:M3K5	GO:0023014 - signal transduction by protein phosphorylation (P)	PMID:26243192	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1
updatedbyinstructor	Christin3sheets, Team Hardy Weinburgers	BACSU:MLEN	GO:0015385 - sodium:proton antiporter activity (F)	PMID:10903309	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1 fixed by Dyd5192

5 annotations fixed by Dyd5192

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