GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:26414402
| Citation |
Roostalu, J, Cade, NI and Surrey, T' (2015) Complementary activities of TPX2 and chTOG constitute an efficient importin-regulated microtubule nucleation module. Nat. Cell Biol. ' |
|---|---|
| Abstract |
Spindle assembly and function require precise control of microtubule nucleation and dynamics. The chromatin-driven spindle assembly pathway exerts such control locally in the vicinity of chromosomes. One of the key targets of this pathway is TPX2. The molecular mechanism of how TPX2 stimulates microtubule nucleation is not understood. Using microscopy-based dynamic in vitro reconstitution assays with purified proteins, we find that human TPX2 directly stabilizes growing microtubule ends and stimulates microtubule nucleation by stabilizing early microtubule nucleation intermediates. Human microtubule polymerase chTOG (XMAP215/Msps/Stu2p/Dis1/Alp14 homologue) only weakly promotes nucleation, but acts synergistically with TPX2. Hence, a combination of distinct and complementary activities is sufficient for efficient microtubule formation in vitro. Importins control the efficiency of the microtubule nucleation by selectively blocking the interaction of TPX2 with microtubule nucleation intermediates. This in vitro reconstitution reveals the molecular mechanism of regulated microtubule formation by a minimal nucleation module essential for chromatin-dependent microtubule nucleation in cells. |
| Links |
PubMed Online version:10.1038/ncb3241 |
| Keywords |
|
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0008017: microtubule binding |
ECO:0000314: |
F |
Fig. 2 C-F show TPX2 binding along tubulin. |
complete | ||||
| GO:0007020: microtubule nucleation |
ECO:0000314: |
P |
Fig 6.b, and Fig 6.c shows microtuble nucleation by TPX2 |
complete | ||||
| GO:0007026: negative regulation of microtubule depolymerization |
ECO:0000314: |
P |
Fig 3f shows reduced depolymerization activity with TPX2. |
complete | ||||
| GO:0090063: positive regulation of microtubule nucleation |
ECO:0000314: |
P |
Figure 6b&c |
complete | ||||
Notes
See also
References
See Help:References for how to manage references in GONUTS.
