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Cacao

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GO:0008237metallopeptidase activityPMID:7854123ISO: Inferred from Sequence Orthology UniProtKB:P15917 F
This annotation made on page: BACAN:LEF
By: Dyd5192 (group Team PSU 1) on 2015-10-14 11:47:27 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeDyd5192,
Team PSU 1
2015-11-04 08:57:10 CST

Evidence code should be changed to ISA: Inferred from sequence alignment.

0
ChallengeDyd5192,
Team PSU 1
2015-11-04 08:49:37 CST

Metallopeptidase activity of LEF is shown in Figure 1 instead of Figure 5 by the DNA sequence comparison of LEF to Thermolysin, Listeria mpl, Elastase, Serratia npr, Tetanus Toxin, Bot D, and Rat apN. They all contain the HEXXH motif that infer the metallopeptidase activity.

0
ChallengeZacharymax,
Team G-nomes
2015-10-21 14:17:14 CDT

The "with" column contains the same protein that is being annotated. Additionally Figure 5 shows levels of zinc binding of LEF and various LEF mutants, not sequence comparison as the notes state. I think this annotation could be fixed by using Figure 1, and changing the evidence code to ISA.

4
ChallengeZacharymax,
Team G-nomes
2015-10-20 22:42:57 CDT

The "with" column contains the same protein that is being annotated. Additionally Figure 5 shows levels of zinc binding of LEF and various LEF mutants, not sequence comparison as the notes state. I think this annotation could be fixed by using Figure 1, and changing the evidence code to ISA.

0
Private
Assessment
Amcmillan2015-11-17 20:19:34 CSTYou need to be an instructor to view these notes.Acceptable
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2015-11-13 10:43:19 CST

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0008237 metallopeptidase activity PMID:7854123 ISA: Inferred from Sequence Alignment UniProtKB:P04958 F Figure 1. Shows DNA sequence comparison of LEF with other proteins from different organisms that present metallopeptidase activity. Lethal factor sequence contain the HEXXH motif inferring its metallopeptidase activity complete
CACAO 11021
on BACAN:LEF
Flagged
Public
Assessment
Suzialeksander2015-11-05 16:34:15 CST

OK, so suggesting the same thing again won't win you anything. Read over how to use that code: http://gowiki.tamu.edu/wiki/index.php/evidence_codes#ISA

You need a With, and there's specific rules about what can be used.

Requires Changes
With/From
Public
Assessment
DanielRenfro2015-11-04 08:57:10 CST

This annotation has been flagged because it has been challenged since last assessment

Flagged
Public
Assessment
DanielRenfro2015-11-04 08:49:37 CST

This annotation has been flagged because it has been challenged since last assessment

Flagged
Private
Assessment
Suzialeksander2015-11-03 13:17:46 CSTYou need to be an instructor to view these notes.Requires Changes
With/From