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Category:Team Sandy Konas

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableSCHPO:XRN2Sandyl 27, Team Sandy Konas2016-04-19 02:24:30 CDTGO:0070870 heterochromatin maintenance involved in chromatin silencing (P)PMID:26889830ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 5E, Interestingly, mutation of dhp1 does not reduce this histone modification at the same region (Fig 5E), suggesting that Dhp1 plays a role in effective maintenance of epigenetic silencing downstream of H3K9me.

challenge
acceptableSCHPO:GAD8Sandyl 27, Team Sandy Konas2016-03-07 21:34:33 CSTGO:0005737 cytoplasm (C)PMID:26912660ECO:0000314 direct assay evidence used in manual assertion

Figure 2

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acceptableSCHPO:TOR2Sandyl 27, Team Sandy Konas2016-03-07 21:28:01 CSTGO:0005634 nucleus (C)PMID:26912660ECO:0000314 direct assay evidence used in manual assertion

Figure 1E

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acceptableHUMAN:SIR3KonaKuaiwa, Team Sandy Konas2016-03-03 01:33:27 CSTGO:0005759 mitochondrial matrix (C)PMID:12186850ECO:0000314 direct assay evidence used in manual assertion

Figure 5

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unacceptableHUMAN:SIR3KonaKuaiwa, Team Sandy Konas2016-03-02 12:31:12 CSTGO:1903206 negative regulation of hydrogen peroxide-induced cell death (P)PMID:25210848ECO:0000316 genetic interaction evidence used in manual assertion

Figure 3J & 3K show that transfection with the SIRT3 expression construct significantly reduces hydrogen peroxide-induced apoptosis by enhancing antioxidant activity of CAT and MnSOD

challenge
updatedbyinstructorHUMAN:SIR3KonaKuaiwa, Team Sandy Konas2016-03-02 12:31:11 CSTGO:1901671 positive regulation of superoxide dismutase activity (P)PMID:25210848ECO:0000314 direct assay evidence used in manual assertion

Figure 2(E-F) shows that pSIRT3 transfection increases gene & protein levels of MnSOD. 2G indicates enzymatic activity of MnSOD is also enhanced.

challenge
updatedbyinstructorHUMAN:SIR3KonaKuaiwa, Team Sandy Konas2016-03-02 12:31:10 CSTGO:1902553 positive regulation of catalase activity (P)PMID:25210848ECO:0000314 direct assay evidence used in manual assertion

Figure 2(H-I) shows that pSIRT3 transfection increases gene & protein levels of CAT. 2J indicates enzymatic activity of CAT is also enhanced.

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unacceptableYEAST:TOR2Sandyl 27, Team Sandy Konas2016-03-01 20:57:37 CSTGO:0005634 nucleus (C)PMID:26912660ECO:0000314 direct assay evidence used in manual assertion

Figure 1E

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acceptableSCHPO:TOR1Sandyl 27, Team Sandy Konas2016-03-01 20:54:16 CSTGO:0000790 nuclear chromatin (C)PMID:26912660ECO:0000314 direct assay evidence used in manual assertion

Fig 1C

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acceptableSCHPO:GAD8Sandyl 27, Team Sandy Konas2016-03-01 20:49:47 CSTGO:0000790 nuclear chromatin (C)PMID:26912660ECO:0000314 direct assay evidence used in manual assertion

Figure 1A

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unacceptableSCHPO:CLR4KonaKuaiwa, Team Sandy Konas2016-03-01 20:05:20 CSTGO:1900111 positive regulation of histone H3-K9 dimethylation (P)PMID:26889830ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2B&C show that levels of H3K9me2 at the reporter genes embedded in these regions were substantially reduced at these loci in cells deficient in dhp1

challenge
unacceptableSCHPO:XRN2KonaKuaiwa, Team Sandy Konas2016-03-01 20:02:26 CSTGO:1900111 positive regulation of histone H3-K9 dimethylation (P)PMID:26889830ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 2 B & C shows that levels of H3K9me2 at the reporter genes embedded in these regions were substantially reduced at these loci in cells deficient in dhp1

challenge
updatedbyinstructorSCHPO:CLR4Sandyl 27, Team Sandy Konas2016-03-01 19:42:10 CSTGO:0070829 heterochromatin maintenance (P)PMID:26889830ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2A

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acceptableSCHPO:XRN2Sandyl 27, Team Sandy Konas2016-03-01 19:39:26 CSTGO:0051984 positive regulation of chromosome segregation (P)PMID:26889830ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2A chromosome segregation defects in dhp1-1 mutants and Supp. 5

challenge
updatedbyinstructorSCHPO:CLR4Sandyl 27, Team Sandy Konas2016-03-01 19:29:50 CSTGO:0030702 chromatin silencing at centromere (P)PMID:26889830ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1B,C Similar to clr4Δ, dhp1-1 but not din1-null (din1Δ) cells form white colonies, indicating a silencing defect at the mating type locus unique to the dhp1 mutant.

challenge
updatedbyinstructorSCHPO:XRN2Sandyl 27, Team Sandy Konas2016-03-01 19:26:24 CSTGO:0030702 chromatin silencing at centromere (P)PMID:26889830ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1B,C dhp1-1 but not din1-null (din1Δ) cells form white colonies, indicating a silencing defect at the mating type locus unique to the dhp1 mutant

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acceptableSCHPO:CSK2BSandyl 27, Team Sandy Konas2016-02-19 15:27:58 CSTGO:0006468 protein phosphorylation (P)PMID:19136623ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 Since Ckb1 is a regulatory subunit of casein kinase II (CK2) CK2 phosphorylates Swi6

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acceptableSCHPO:SWI6Sandyl 27, Team Sandy Konas2016-02-19 15:12:46 CSTGO:0090053 positive regulation of chromatin silencing at centromere (P)PMID:19136623ECO:0000315 mutant phenotype evidence used in manual assertion

silencing defects in ckb1Δ cells was comparable with that caused by disruption of other heterochromatic genes, swi6 (Fig. 1B).

challenge
acceptableSCHPO:CLR3Sandyl 27, Team Sandy Konas2016-02-19 15:09:08 CSTGO:0090053 positive regulation of chromatin silencing at centromere (P)PMID:19136623ECO:0000315 mutant phenotype evidence used in manual assertion

silencing defects in ckb1Δ cells was comparable with that caused by disruption of other heterochromatic genes, including clr3, (Fig. 1B).

challenge
acceptableSCHPO:CLR4Sandyl 27, Team Sandy Konas2016-02-19 15:05:53 CSTGO:0090053 positive regulation of chromatin silencing at centromere (P)PMID:19136623ECO:0000315 mutant phenotype evidence used in manual assertion

silencing defects in ckb1Δ cells was comparable with that caused by disruption of other heterochromatic genes, including clr4,Fig. 1B).

challenge
updatedbyinstructorSCHPO:CSK2BSandyl 27, Team Sandy Konas2016-02-19 14:58:52 CSTGO:0090053 positive regulation of chromatin silencing at centromere (P)PMID:19136623ECO:0000315 mutant phenotype evidence used in manual assertion

disruption of ckb1 alleviated the silencing of marker genes inserted in centromeric heterochromatin (Fig. 1B)

challenge
unacceptableSCHPO:ALG2Sandyl 27, Team Sandy Konas2016-02-19 14:21:06 CSTGO:0031965 nuclear membrane (C)PMID:22156748ECO:0000314 direct assay evidence used in manual assertion

We verified the localization of the fusion protein by

fluorescence microscopy and found it to be present mainly in the nuclear membrane (Fig. 1A)

challenge
acceptableSCHPO:LEM2Sandyl 27, Team Sandy Konas2016-02-16 20:37:52 CSTGO:0140462 pericentric heterochromatin organization (P)PMID:26744419ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1C Quantitative analysis by RT-qPCR revealed that lem2Δ cells display a twofold increase in reporter gene expression

needs relation

challenge
acceptableSCHPO:CAPSandyl 27, Team Sandy Konas2016-02-09 14:53:26 CSTGO:0030308 negative regulation of cell growth (P)PMID:26542710ECO:0000316 genetic interaction evidence used in manual assertion

Figure 5

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acceptableSCHPO:CAPSandyl 27, Team Sandy Konas2016-02-09 14:47:35 CSTGO:0034613 cellular protein localization (P)PMID:26542710ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6 this protein is keeping csh3 in the right place

challenge
acceptableSCHPO:CSH3Sandyl 27, Team Sandy Konas2016-02-09 14:39:04 CSTGO:0005737 cytoplasm (C)PMID:26542710ECO:0000314 direct assay evidence used in manual assertion

Figure 6. Following transformation, Csh3-GFP localized to the cytoplasm,

challenge
acceptableSCHPO:CAPSandyl 27, Team Sandy Konas2016-02-05 11:48:57 CSTGO:0030428 cell septum (C)PMID:26542710ECO:0000314 direct assay evidence used in manual assertion

Figure 6 Cap1-GFP localized to cortical patches at the growing ends or septum

challenge
acceptableSCHPO:CAPSandyl 27, Team Sandy Konas2016-02-05 11:42:01 CSTGO:0007015 actin filament organization (P)PMID:26542710ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 7 actin patches were not properly formed in cap1Δ

challenge
updatedbyinstructorSCHPO:SGO2Sandyl 27, Team Sandy Konas2016-02-04 16:39:20 CSTGO:1903467 negative regulation of mitotic DNA replication initiation (P)PMID:26804021ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 7

challenge
acceptablePIG:HMGB2Sandyl 27, Team Sandy Konas2016-02-02 19:25:54 CSTGO:1904877 positive regulation of DNA ligase activity (P)PMID:9600082ECO:0000314 direct assay evidence used in manual assertion

Figure 4

challenge
acceptablePIG:HMGB1Sandyl 27, Team Sandy Konas2016-02-02 19:20:35 CSTGO:1904877 positive regulation of DNA ligase activity (P)PMID:9600082ECO:0000314 direct assay evidence used in manual assertion

Figure 2

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updatedbyinstructorSCHPO:SGO2Sandyl 27, Team Sandy Konas2016-02-02 18:07:31 CSTGO:1990421 subtelomeric heterochromatin (C)PMID:26804021ECO:0000314 direct assay evidence used in manual assertion

ChIP–chip analysis of the genome-wide distribution of Sgo2-Flag in logarithmically growing wt cells. It is noteworthy that the probes used in the tiling array did not encompass regions outside the rDNA repeats on chromosome 3. (c) Magnification of the ChIP–chip data for the subtelomeric regions shown in b.

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Pages in category "Team Sandy Konas"

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