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Category:Team You cannot B. cereus

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableCOXBU:Q83F76Annie luca, Team You cannot B. cereus2015-11-24 17:10:56 CSTGO:0044164 host cell cytosol (C)PMID:19411324ECO:0000314 direct assay evidence used in manual assertion

FIG. 6. AnkA-mCherry localized diffusely throughout the host cytoplasm.

challenge
unacceptableCOXBU:Y781Annie luca, Team You cannot B. cereus2015-11-24 17:04:39 CSTGO:0052048 interaction with host via secreted substance involved in symbiotic interaction (P)PMID:19411324ECO:0000314 direct assay evidence used in manual assertion

FIG. 3. Multiple C. burnetii Anks are translocated into the host

cytosol in a Dot/Icm-dependent manner (AnkG).

challenge
unacceptableCOXBU:Q83E81Annie luca, Team You cannot B. cereus2015-11-24 17:01:03 CSTGO:0052048 interaction with host via secreted substance involved in symbiotic interaction (P)PMID:19411324ECO:0000314 direct assay evidence used in manual assertion

FIG. 3. Multiple C. burnetii Anks are translocated into the host

cytosol in a Dot/Icm-dependent manner (AnkF).

challenge
unacceptableCOXBU:Q83F76Annie luca, Team You cannot B. cereus2015-11-24 16:56:45 CSTGO:0052048 interaction with host via secreted substance involved in symbiotic interaction (P)PMID:19411324ECO:0000314 direct assay evidence used in manual assertion

FIG. 3. Multiple C. burnetii Anks are translocated into the host

cytosol in a Dot/Icm-dependent manner (AnkA).

challenge
unacceptableBRUME:VJBRAnnie luca, Team You cannot B. cereus2015-11-24 15:37:05 CSTGO:0044144 modulation of growth of symbiont involved in interaction with host (P)PMID:16008582ECO:0000314 direct assay evidence used in manual assertion

Fig. 1. Virulence of B. melitensis 16M vjbR mutant.

(A) Intracellular replication of B. melitensis 16M NalR (WT), vjbR mutant (DvjbR or vjbR::mTn5) and complemented vjbR mutant (DvjbRc) in HeLa cells or bovine macrophages. Data (B) Intracellular growth phenotypes of WT, mutant vjbR and complemented mutant vjbR in bovine macrophages cells at 48 h p.i. (C) Accelerated clearance of the vjbR mutant from experimentally infected BALB/c mice.

challenge
updatedbyinstructorBRUME:Q8YHR0Annie luca, Team You cannot B. cereus2015-11-24 14:44:12 CSTGO:0044164 host cell cytosol (C)PMID:21501366ECO:0000314 direct assay evidence used in manual assertion

Fig. 2. Cytosolic localization of HA-tagged RicA

challenge
unacceptableBRUA2:Q2YQG1Annie luca, Team You cannot B. cereus2015-11-24 14:39:57 CSTGO:0052050 interaction with host via substance secreted by type IV secretion system (P)PMID:21501366ECO:0000314 direct assay evidence used in manual assertion

Figure 3. TEM-b-lactamase-RicA translocation into RAW264.7 macrophages.

Fig. 4. VirB-independent secretion of 3Flag-RicA by B. abortus.

challenge
acceptableBRUA2:Q2YS16Annie luca, Team You cannot B. cereus2015-11-24 14:07:02 CSTGO:0052050 interaction with host via substance secreted by type IV secretion system (P)PMID:24119283ECO:0000314 direct assay evidence used in manual assertion

Figure 1. (B & D) The protein product of Bab1_1492 (SepA) is secreted during the infection of host cells.

Figure 2. Confocal analysis of the secretion of the protein product of Bab1_1492 (SepA) Figure 3. Secretion of SepA is a virB dependent process.

challenge
unacceptableBRUA2:Q2YN91Annie luca, Team You cannot B. cereus2015-11-18 09:51:38 CSTGO:0052050 interaction with host via substance secreted by type IV secretion system (P)PMID:23847770ECO:0000314 direct assay evidence used in manual assertion

Figure 3. (A-D) BtpB (BAB1_0756) is translocated into host cells during infection.

challenge
updatedbyinstructorBRUA2:Q2YN91Annie luca, Team You cannot B. cereus2015-11-18 09:45:30 CSTGO:0052170 negative regulation by symbiont of host innate immune response (P)PMID:23847770ECO:0000314 direct assay evidence used in manual assertion

BtpB (BAB1_0756); Figure 5. Modulation of NF-κB translocation to the nucleus during Brucella infection. BtpB mutant showed an increase translocation of NF-kB into the nucleus compared to those infected with Brucella WT.

challenge
unacceptableBRUSU:A0A0H3GG90Annie luca, Team You cannot B. cereus2015-11-17 14:53:25 CSTGO:0052050 interaction with host via substance secreted by type IV secretion system (P)PMID:19019140ECO:0000314 direct assay evidence used in manual assertion

Figure 7. Translocation of TEM1::VceA and TEM1::VceC into J774 macrophages. Cytosolic translocation of b-lactamase by wild type (2308) or virB2 mutant strain (ADH3) of B. abortus was assessed by fluorescence microscopy. Cells in which translocation of the fusion protein has occurred appear blue. Shows translocation of effector protein VceC (BR1038) from Brucella suis strain 1330 by VirB-T4SS.

Figure 8. Translocation of TEM1::VceA and TEM1::VceC into J774 macrophages at different time points after infection. Shows translocation of effector protein VceC (BAR1038) from Brucella suis strain 1330 by VirB-T4SS during Brucella abortus infection.

challenge
updatedbyinstructorBACSU:FLIWSandyl 27, Team You cannot B. cereus2015-11-02 14:46:19 CSTGO:0045727 positive regulation of translation (P)PMID:21895793ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2C

challenge
updatedbyinstructorBACSU:FLIWSandyl 27, Team You cannot B. cereus2015-11-02 14:46:18 CSTGO:0071978 bacterial-type flagellum-dependent swarming motility (P)PMID:21895793ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1B

challenge
acceptableBACSU:FLIWSandyl 27, Team You cannot B. cereus2015-11-02 14:46:18 CSTGO:1902210 positive regulation of bacterial-type flagellum assembly (P)PMID:21895793ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2A

challenge
acceptableBACSU:FLIWSandyl 27, Team You cannot B. cereus2015-11-02 14:30:03 CSTGO:0071978 bacterial-type flagellum-dependent swarming motility (P)PMID:16936039ECO:0000315 mutant phenotype evidence used in manual assertion

Figure3

challenge
acceptableBACSU:FLIWSandyl 27, Team You cannot B. cereus2015-11-02 14:28:45 CSTGO:1902021 regulation of bacterial-type flagellum-dependent cell motility (P)PMID:16936039ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3

challenge
updatedbyinstructorDICDI:PDPKASandyl 27, Team You cannot B. cereus2015-10-20 12:43:29 CDTGO:0023014 signal transduction by protein phosphorylation (P)PMID:20200230ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4

challenge
updatedbyinstructorDICDI:PDPKASandyl 27, Team You cannot B. cereus2015-10-19 15:57:07 CDTGO:0098630 aggregation of unicellular organisms (P)PMID:20200230ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4B,C

challenge
unacceptableYEAST:SET1Sandyl 27, Team You cannot B. cereus2015-10-19 15:40:08 CDTGO:0070452 positive regulation of ergosterol biosynthetic process (P)PMID:23382196ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 Figure 4

challenge
unacceptableYEAST:SET3Sandyl 27, Team You cannot B. cereus2015-10-19 15:38:42 CDTGO:0070452 positive regulation of ergosterol biosynthetic process (P)PMID:23382196ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6

challenge
unacceptableDICDI:Q54IA6Sandyl 27, Team You cannot B. cereus2015-10-19 15:22:11 CDTGO:0006468 protein phosphorylation (P)PMID:20200230ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1D

challenge
acceptableDICDI:PK2Sandyl 27, Team You cannot B. cereus2015-10-19 15:21:04 CDTGO:0006468 protein phosphorylation (P)PMID:20200230ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1E

challenge
acceptableDICDI:KRACSandyl 27, Team You cannot B. cereus2015-10-19 15:18:44 CDTGO:0006468 protein phosphorylation (P)PMID:20200230ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1D

challenge
acceptableCOXBU:Q83ED7Annie luca, Team You cannot B. cereus2015-10-19 14:05:32 CDTGO:0042025 host cell nucleus (C)PMID:23813730ECO:0000314 direct assay evidence used in manual assertion

Figure 2. C. burnetii secretion substrates target distinct subcellular compartments when ectopically expressed in HeLa cells. Colocalization of Cbu0388 with the host cell nucleus could be demonstrated.

challenge
acceptableCOXBU:Q83A08Annie luca, Team You cannot B. cereus2015-10-19 13:59:38 CDTGO:0033650 host cell mitochondrion (C)PMID:23813730ECO:0000314 direct assay evidence used in manual assertion

Figure 2B. C. burnetii secretion substrates target distinct subcellular compartments when ectopically expressed in HeLa cells. CbuA0020 colocalizes with the mitochondrial marker CoxIV (arrow indication).

challenge
acceptableCOXBU:Q83BD5Annie luca, Team You cannot B. cereus2015-10-19 13:50:44 CDTGO:0044165 host cell endoplasmic reticulum (C)PMID:23813730ECO:0000314 direct assay evidence used in manual assertion

Figure 2B. C. burnetii secretion substrates target distinct subcellular compartments when ectopically expressed in HeLa cells. Cbu1576 colocalization with the endoplasmic reticulum marker calnexin (arrow indication).

challenge
acceptableCOXBU:Q83EF1Annie luca, Team You cannot B. cereus2015-10-19 13:48:41 CDTGO:0044165 host cell endoplasmic reticulum (C)PMID:23813730ECO:0000314 direct assay evidence used in manual assertion

Figure 2B. C. burnetii secretion substrates target distinct subcellular compartments when ectopically expressed in HeLa cells. Cbu0372 colocalizes with

the endoplasmic reticulum marker calnexin (arrow indication)

challenge
unacceptableBRUA2:Q2YQ34Annie luca, Team You cannot B. cereus2015-10-19 11:36:45 CDTGO:0034976 response to endoplasmic reticulum stress (P)PMID:23422410ECO:0000314 direct assay evidence used in manual assertion

Figure 6 and 7. Shows that VceC produces ER stress.

challenge
acceptableBRUA2:Q2YQ34Annie luca, Team You cannot B. cereus2015-10-19 11:12:29 CDTGO:0044165 host cell endoplasmic reticulum (C)PMID:23422410ECO:0000314 direct assay evidence used in manual assertion

Figure 4. Localization of HA-VceC (BAB1_1058) fusions in HeLa cells (A to C) and RAW264.7 macrophages (D). Transfected cells grown on coverslips were stained with anti-calreticulin antibody (red) to visualize the ER (A, B, and D)or with phalloidin (red) to visualize the actin cytoskeleton (C). HA-VceC was visualized with anti-HA antibody (green). The images shown are representative of at least three independent experiments.

challenge
unacceptableBRUA2:Q2YRI6Annie luca, Team You cannot B. cereus2015-10-19 10:43:55 CDTGO:0052050 interaction with host via substance secreted by type IV secretion system (P)PMID:19019140ECO:0000314 direct assay evidence used in manual assertion

Figure 7. Translocation of TEM1::VceA and TEM1::VceC into J774 macrophages. Cytosolic translocation of b-lactamase by wild type (2308) or virB2 mutant strain (ADH3) of B. abortus was assessed by fluorescence microscopy. Cells in which translocation of the fusion protein has occurred appear blue. Shows translocation of effector protein VceA (BAB1_1652) by VirB-T4SS.

Figure 8. Translocation of TEM1::VceA and TEM1::VceC into J774 macrophages at different time points after infection. Shows translocation of effector protein VceA (BAB1_1652) by VirB-T4SS during Brucella abortus infection.

challenge
unacceptableBRUA2:Q2YQ34Annie luca, Team You cannot B. cereus2015-10-19 10:40:53 CDTGO:0052050 interaction with host via substance secreted by type IV secretion system (P)PMID:19019140ECO:0000314 direct assay evidence used in manual assertion

Figure 7. Translocation of TEM1::VceA and TEM1::VceC into J774 macrophages. Cytosolic translocation of b-lactamase by wild type (2308) or virB2 mutant strain (ADH3) of B. abortus was assessed by fluorescence microscopy. Cells in which translocation of the fusion protein has occurred appear blue. Shows translocation of effector protein VceC (BAB1_1058) by VirB-T4SS.

Figure 8. Translocation of TEM1::VceA and TEM1::VceC into J774 macrophages at different time points after infection. Shows translocation of effector protein VceC (BAB1_1058) by VirB-T4SS during Brucella abortus infection.

challenge
unacceptableBRUA2:Q2YLD2Annie luca, Team You cannot B. cereus2015-10-18 21:03:34 CDTGO:0052050 interaction with host via substance secreted by type IV secretion system (P)PMID:21707904ECO:0000314 direct assay evidence used in manual assertion

Figure 2. Effector proteins (BPE's) translocation into J774.A1. Shows translocation of BPE123 (BAB2_0123) into host cell.

Figure 3. VirB-dependent translocation of effector proteins (BPE's) into J774.A1 cells. Shows translocation of BPE123 (BAB2_0123) dependent on VirB-T4SS.

challenge
unacceptableBRUA2:Q2YQG9Annie luca, Team You cannot B. cereus2015-10-18 20:59:36 CDTGO:0052050 interaction with host via substance secreted by type IV secretion system (P)PMID:21707904ECO:0000314 direct assay evidence used in manual assertion

Figure 2. Effector proteins (BPE's) translocation into J774.A1. Shows translocation of BPE275 (BAB1_1275) into host cell.

Figure 3. VirB-dependent translocation of effector proteins (BPE's) into J774.A1 cells. Shows translocation of BPE275 (BAB1_1275) dependent on VirB-T4SS.

challenge
unacceptableBRUA2:Q2YJ64Annie luca, Team You cannot B. cereus2015-10-18 20:56:34 CDTGO:0052050 interaction with host via substance secreted by type IV secretion system (P)PMID:21707904ECO:0000314 direct assay evidence used in manual assertion

Figure 2. Effector proteins (BPE's) translocation into J774.A1. Shows translocation of BPE 159 (BAB2_0159) into host cell.

Figure 3. VirB-dependent translocation of effector proteins (BPE's) into J774.A1 cells. Shows translocation of BPE 159 (BAB2_0159) dependent on VirB-T4SS.

challenge
unacceptableBRUA2:Q2YQ47Annie luca, Team You cannot B. cereus2015-10-18 20:50:21 CDTGO:0052050 interaction with host via substance secreted by type IV secretion system (P)PMID:21707904ECO:0000314 direct assay evidence used in manual assertion

Figure 2. Effector proteins (BPE's) translocation into J774.A1. Shows translocation of BPE043 (BAB1_1043) into host cell.

Figure 3. VirB-dependent translocation of effector proteins (BPE's) into J774.A1 cells. Shows translocation of BPE043 (BAB1_1043) dependent on VirB-T4SS.

challenge
unacceptableBRUA2:Q2YR64Annie luca, Team You cannot B. cereus2015-10-18 20:46:56 CDTGO:0052050 interaction with host via substance secreted by type IV secretion system (P)PMID:21707904ECO:0000314 direct assay evidence used in manual assertion

Figure 2. Effector proteins (BPE's) translocation into J774.A1. Shows translocation of BPE005 (BAB1_2005) into host cell.

Figure 3. VirB-dependent translocation of effector proteins (BPE's) into J774.A1 cells. Shows translocation of BPE005 (BAB1_2005) dependent on VirB-T4SS.

challenge
unacceptableBRUA2:Q2YLL0Annie luca, Team You cannot B. cereus2015-10-18 20:43:45 CDTGO:0052050 interaction with host via substance secreted by type IV secretion system (P)PMID:21707904ECO:0000314 direct assay evidence used in manual assertion

Figure 2. Effector proteins (BPE's) translocation into J774.A1. Shows translocation of BPE865 (BAB1_1865) into host cell.

Figure 3. VirB-dependent translocation of effector proteins (BPE's) into J774.A1 cells. Shows translocation of BPE865 (BAB1_1865) dependent on VirB-T4SS.

challenge
acceptableYEAST:SET1Sandyl 27, Team You cannot B. cereus2015-10-13 20:00:37 CDTGO:1990872 negative regulation of sterol import by negative regulation of transcription from RNA polymerase II promoter (P)PMID:23382196ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5

challenge
acceptableYEAST:SET1Sandyl 27, Team You cannot B. cereus2015-10-13 19:54:37 CDTGO:0055092 sterol homeostasis (P)PMID:23382196ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4

challenge
acceptableYEAST:SET3Sandyl 27, Team You cannot B. cereus2015-10-13 19:52:26 CDTGO:0035961 positive regulation of ergosterol biosynthetic process by positive regulation of transcription from RNA polymerase II promoter (P)PMID:23382196ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6

challenge
acceptableHUMAN:CKAP5Sandyl 27, Team You cannot B. cereus2015-10-13 18:07:43 CDTGO:0090063 positive regulation of microtubule nucleation (P)PMID:26414402ECO:0000314 direct assay evidence used in manual assertion

Figure 6b&c

challenge
unacceptableDICDI:ERK2Sandyl 27, Team You cannot B. cereus2015-10-06 12:44:28 CDTGO:0004713 protein tyrosine kinase activity (F)PMID:9164876ECO:0000314 direct assay evidence used in manual assertion

Figure 2

cAMP-induced tyrosine phosphorylation of ERK2 in Ax2 cells

challenge
acceptableECOLI:FKBASandyl 27, Team You cannot B. cereus2015-10-05 15:35:37 CDTGO:0003755 peptidyl-prolyl cis-trans isomerase activity (F)PMID:10748201ECO:0000314 direct assay evidence used in manual assertion

Figure 2: Fluorescence traces of refolding of RNase T1 (0.2 μM) at 10 °C starting from equilibrium-denatured protein (trace a) in the absence or presence of 0.5 nM (trace b), 2 nM (trace c), and 5 nM (trace d) FkpA (calculated for the dimer). The intensity at 323 nm was followed. Inset, the refolding curves were evaluated with a double exponential fit; and the rates of the faster refolding phase, divided by the uncatalyzed rate, are plotted against the respective FkpA concentration present during the measurement. arb., arbitrary.

challenge
unacceptableBRUA2:Q2YKV5Annie luca, Team You cannot B. cereus2015-10-04 22:16:25 CDTGO:0034976 response to endoplasmic reticulum stress (P)PMID:23950720ECO:0000314 direct assay evidence used in manual assertion

Figure S1. Panel B and C shows that, when ectopically expressed in mammalian cells (HEK 293T), BAB2_0541 expression induces significant ER stress to levels similar or higher than a positive control VceC and upregulation of BiP, but not CHOP.

challenge
unacceptableBRUA2:Q2YP75Annie luca, Team You cannot B. cereus2015-10-04 22:14:59 CDTGO:0034976 response to endoplasmic reticulum stress (P)PMID:23950720ECO:0000314 direct assay evidence used in manual assertion

Figure S1. Panel B and C shows that, when ectopically expressed in mammalian cells (HEK 293T), BAB1_0227 expression induces significant ER stress to levels similar or higher than a positive control VceC and upregulation of BiP, but not CHOP.

challenge
unacceptableBRUA2:Q2YNH3Annie luca, Team You cannot B. cereus2015-10-04 22:13:19 CDTGO:0034976 response to endoplasmic reticulum stress (P)PMID:23950720ECO:0000314 direct assay evidence used in manual assertion

Figure S1. Panel B and C shows that, when ectopically expressed in mammalian cells (HEK 293T), BAB1_0847 expression induces significant ER stress to levels similar or higher than a positive control VceC and upregulation of BiP, but not CHOP.

challenge
acceptableBRUA2:Q2YLT8Annie luca, Team You cannot B. cereus2015-10-04 22:04:19 CDTGO:0052208 modification of morphology or physiology of other organism via substance secreted by type IV secretion system involved in symbiotic interaction (P)PMID:23950720ECO:0000314 direct assay evidence used in manual assertion

Figure 1. TEM1 b-lactamase protein translocation reporter assay. Shows translocation of effector protein BspF (BAB1_1948)

Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells. Shows effector protein BspF (BAB1_01948) translocated by T4SS.

Figure 4. A subset of Bsp effectors target the host secretory pathway. Shows that effector protein BspF (BAB1_1948) targets secretory pathway by colocalization with calnexin a marker of ER.

Figure 5. Ectopic expression of BspF (BAB1_1948) in HeLa cells interferes with the host secretory pathway.

Figure 7. Contribution of BspF (BAB1_1948) to inhibition of host cell secretion during infection.

challenge
unacceptableBRUA2:Q2YRH9Annie luca, Team You cannot B. cereus2015-10-04 22:03:35 CDTGO:0042000 translocation of peptides or proteins into host (P)PMID:23950720ECO:0000314 direct assay evidence used in manual assertion

Figure 1. TEM1 b-lactamase protein translocation reporter assay.

Panel A and B shows percentage of shift in fluorescence emission that correlates with protein Brucella putative effector BAB1_1671 being translocated into 774A.1 host cell (murine cell line). Panel C and D shows representative fluorescence micrographs of protein translocation. Also look Figure S1 and S2.

Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells.

Panel A. B. abortus strain 2308 (black bars) or virB9 mutant strain (white bars) expressing the CyaA fusions to the indicated Brucella effector proteins were used to infect J774.A1 cells, and the cAMP level of infected cells was determined.

challenge
unacceptableBRUA2:Q2YNH3Annie luca, Team You cannot B. cereus2015-10-04 22:02:43 CDTGO:0042000 translocation of peptides or proteins into host (P)PMID:23950720ECO:0000314 direct assay evidence used in manual assertion

Figure 1. TEM1 b-lactamase protein translocation reporter assay.

Panel A and B shows percentage of shift in fluorescence emission that correlates with protein Brucella putative effector BAB1_0847 being translocated into J774A.1 host cell (murine cell line). Panel C and D shows representative fluorescence micrographs of protein translocation. Also look Figure S1 and S2.

Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells.

Panel A. B. abortus strain 2308 (black bars) or virB9 mutant strain (white bars) expressing the CyaA fusions to the indicated Brucella effector proteins were used to infect J774.A1 cells, and the cAMP level of infected cells was determined.

challenge
updatedbyinstructorBRUA2:Q2YN05Annie luca, Team You cannot B. cereus2015-10-04 22:01:51 CDTGO:0052058 modification by symbiont of host morphology or physiology via substance secreted by type IV secretion system (P)PMID:23950720ECO:0000314 direct assay evidence used in manual assertion

Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells. Shows effector protein BspA (BAB1_0678) translocated by T4SS.

Figure 7. Contribution of BspA (BAB1_0678) to inhibition of host cell secretion during infection.

challenge
updatedbyinstructorBRUA2:Q2YN43Annie luca, Team You cannot B. cereus2015-10-04 22:01:11 CDTGO:0052058 modification by symbiont of host morphology or physiology via substance secreted by type IV secretion system (P)PMID:23950720ECO:0000314 direct assay evidence used in manual assertion

Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells. Shows effector protein BspB (BAB1_0712) translocated by T4SS.

Figure 7. Contribution of BspB (BAB1_0712) to inhibition of host cell secretion during infection.

challenge
updatedbyinstructorHUMAN:MUS81Sandyl 27, Team You cannot B. cereus2015-09-29 21:06:18 CDTGO:0033687 osteoblast proliferation (P)PMID:26415217ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4B,C

challenge
acceptableYEAST:SET1Sandyl 27, Team You cannot B. cereus2015-09-29 19:44:06 CDTGO:0035961 positive regulation of ergosterol biosynthetic process by positive regulation of transcription from RNA polymerase II promoter (P)PMID:23382196ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2

challenge
acceptableBRUA2:Q2YNH3Annie luca, Team You cannot B. cereus2015-09-22 16:13:54 CDTGO:0044177 host cell Golgi apparatus (C)PMID:23950720ECO:0000314 direct assay evidence used in manual assertion

Figure 3. Confocal micrographs of HeLa cells transfected with Brucella abortus effector protein BspC expressing GFP displayed a golgi apparatus localization.

Figure 4. Shows co-localization of BspC with a protein resident in golgi GM130 that confirmed BspC golgi localization.

challenge
acceptableBRUA2:Q2YKV5Annie luca, Team You cannot B. cereus2015-09-22 16:10:22 CDTGO:0044165 host cell endoplasmic reticulum (C)PMID:23950720ECO:0000314 direct assay evidence used in manual assertion

Figure 3. Confocal micrographs of HeLa cells transfected with Brucella abortus effector protein BspK expressing hemagglutinin displayed a ER localization.

Figure 4. Shows co-localiztion of BspK with ER-resident chaperone, calnexin that confirmed its ER localization.

challenge
acceptableBRUA2:Q2YQA7Annie luca, Team You cannot B. cereus2015-09-22 16:07:12 CDTGO:0044165 host cell endoplasmic reticulum (C)PMID:23950720ECO:0000314 direct assay evidence used in manual assertion

Figure 3. Confocal micrographs of HeLa cells transfected with Brucella abortus effector protein BspD expressing GFP displayed a ER localization.

Figure 4. Shows co-localization of BspD with ER-resident chaperon, calnexin confirming its localization.

challenge
acceptableBRUA2:Q2YN43Annie luca, Team You cannot B. cereus2015-09-22 16:04:06 CDTGO:0044165 host cell endoplasmic reticulum (C)PMID:23950720ECO:0000314 direct assay evidence used in manual assertion

Figure 3. Confocal micrographs of HeLa cells transfected with Brucella abortus effector protein BspB expressing hemagglutinin displayed a localization in the ER.

Figure 4. Shows co_localization of BspB with ER-resident chaperone protein calnexin confirmed its ER localization.

challenge
acceptableBRUA2:Q2YLT8Annie luca, Team You cannot B. cereus2015-09-22 15:57:15 CDTGO:0020002 host cell plasma membrane (C)PMID:23950720ECO:0000314 direct assay evidence used in manual assertion

Figure 3. Confocal micrographs of HeLa cells transfected with Brucella abortus effector protein BspF expressing hemagglutinin displayed a host plasma membrane localization.

challenge
acceptableBRUA2:Q2YLT8Annie luca, Team You cannot B. cereus2015-09-22 15:53:14 CDTGO:0044164 host cell cytosol (C)PMID:23950720ECO:0000314 direct assay evidence used in manual assertion

Figure 3. Confocal micrographs of HeLa cells transfected with Brucella abortus effector protein BspF expressing hemagglutinin displayed a cytosol localization

challenge
acceptableBRUA2:Q2YLL0Annie luca, Team You cannot B. cereus2015-09-22 15:48:46 CDTGO:0044164 host cell cytosol (C)PMID:23950720ECO:0000314 direct assay evidence used in manual assertion

Figure 3. Confocal micrographs of HeLa cells transfected with Brucella abortus effector protein BspI expressing hemagglutinin displayed cytosolic localization.

challenge
acceptableBRUA2:Q2YLL1Annie luca, Team You cannot B. cereus2015-09-22 15:45:28 CDTGO:0044164 host cell cytosol (C)PMID:23950720ECO:0000314 direct assay evidence used in manual assertion

Figure 3. Confocal micrgraphs of HeLa transfected cells with Brucella abortus effector proten BspH expressing hemagglutinin display a cytosolic localization

challenge
acceptableBRUA2:Q2YN05Annie luca, Team You cannot B. cereus2015-09-22 15:39:39 CDTGO:0044165 host cell endoplasmic reticulum (C)PMID:23950720ECO:0000314 direct assay evidence used in manual assertion

Figure 3. Confocal micrographs of HeLa cells transfected with Brucella abortus effector protein BspA expressing GFP. BspA localizes in the host ER.

Figure 4. Shows co-localization of BspA with ER resident chaperone protein calnexin.

challenge
acceptableBRUA2:Q2YRH9Annie luca, Team You cannot B. cereus2015-09-22 15:29:30 CDTGO:0044220 host cell perinuclear region of cytoplasm (C)PMID:23950720ECO:0000314 direct assay evidence used in manual assertion

Figure 3. Confocal micrographs of HeLa cells transfected with Brucella abortus effector protein BspE labeled with HA. BspE was observed forming discrete vesicles in the perinuclear area.

challenge
acceptableYEAST:KPYK1Sandyl 27, Team You cannot B. cereus2015-09-22 12:55:45 CDTGO:0004743 pyruvate kinase activity (F)PMID:21907146ECO:0000316 genetic interaction evidence used in manual assertion

Figure 1B and 1C

Pyruvate kinase activity of TEFpr-PYK1 and CYCpr-PYK1.

TEFpr is a strong promotor, CYCpr is a weak promotor.

challenge
acceptableYEAST:KPYK2Sandyl 27, Team You cannot B. cereus2015-09-22 12:55:02 CDTGO:0004743 pyruvate kinase activity (F)PMID:21907146ECO:0000316 genetic interaction evidence used in manual assertion

Figure 1B and C

Pyruvate kinase activity of TEFpr-PYK2 and CYCpr-PYK2. TEFpr is a strong promotor, CYCpr is a weak promotor.

challenge
unacceptableYEAST:KPYK1Sandyl 27, Team You cannot B. cereus2015-09-21 14:38:02 CDTGO:0006098 pentose-phosphate shunt (P)PMID:21907146ECO:0000314 direct assay evidence used in manual assertion

Figure 5B

Cells with low PYK activity have increased concentrations of PPP intermediates

challenge
unacceptableYEAST:KPYK2Sandyl 27, Team You cannot B. cereus2015-09-21 14:36:52 CDTGO:0006098 pentose-phosphate shunt (P)PMID:21907146ECO:0000314 direct assay evidence used in manual assertion

Figure 5B

Cells with low PYK activity have increased concentrations of PPP intermediates

challenge
acceptableHUMAN:GRDNSandyl 27, Team You cannot B. cereus2015-09-15 20:29:21 CDTGO:0051496 positive regulation of stress fiber assembly (P)PMID:16139227ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4B and 5C

challenge

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