PMID:23970550

Citation	Chen, C, Krishnan, V, Macon, K, Manne, K, Narayana, SV and Schneewind, O (2013) Secreted proteases control autolysin-mediated biofilm growth of Staphylococcus aureus. J. Biol. Chem. 288:29440-52
Abstract	Staphylococcus epidermidis, a commensal of humans, secretes Esp protease to prevent Staphylococcus aureus biofilm formation and colonization. Blocking S. aureus colonization may reduce the incidence of invasive infectious diseases; however, the mechanism whereby Esp disrupts biofilms is unknown. We show here that Esp cleaves autolysin (Atl)-derived murein hydrolases and prevents staphylococcal release of DNA, which serves as extracellular matrix in biofilms. The three-dimensional structure of Esp was revealed by x-ray crystallography and shown to be highly similar to that of S. aureus V8 (SspA). Both atl and sspA are necessary for biofilm formation, and purified SspA cleaves Atl-derived murein hydrolases. Thus, S. aureus biofilms are formed via the controlled secretion and proteolysis of autolysin, and this developmental program appears to be perturbed by the Esp protease of S. epidermidis.
Links	PubMed PMC3795244 Online version:10.1074/jbc.M113.502039
Keywords	Antibiosis/physiology; Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; Biofilms/growth & development; Crystallography, X-Ray; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; Electrophoresis, Polyacrylamide Gel; Humans; Models, Molecular; N-Acetylmuramoyl-L-alanine Amidase/genetics; N-Acetylmuramoyl-L-alanine Amidase/metabolism; Nasal Cavity/microbiology; Protein Structure, Secondary; Protein Structure, Tertiary; Serine Proteases/chemistry; Serine Proteases/metabolism; Staphylococcal Infections/microbiology; Staphylococcus aureus/genetics; Staphylococcus aureus/metabolism; Staphylococcus aureus/physiology; Staphylococcus epidermidis/enzymology; Staphylococcus epidermidis/physiology; Substrate Specificity
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
STAEP:GSEA		GO:0008233: peptidase activity	ECO:0000315:		F		Figure 1C. Esp activity assay was performed and enzymatic activity was measured. Figure 1D. purified pro-Esp, Esp, or EspS235A was incubated with S. aureus Newman during assembly of biofilms and graph shoes considerable reduction on biofilm formation for Esp sample. In conclusion, purified, recombinant Esp displays protease activity, inhibiting S. aureus biofilm formation and cleaving Atl	complete CACAO 11144
STAEP:GSEA		GO:0009405: pathogenesis	ECO:0000315:		P		Figure 6. Shows that Esp blocks the release of extracellular DNA during biofilm formation	complete CACAO 11148

References

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