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User:Messanyn

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableECOLI:FABF2013-04-11 11:38:52 CDTGO:0033817 beta-ketoacyl-acyl-carrier-protein synthase II activity (F)PMID:3549687ECO:0000314 direct assay evidence used in manual assertion

Figures 1, 2, and 3 show that the fabF mutant (fabF1) activity is decreased by expressing F-ACP. The decrease in activity can be seen by the banding pattern seen after running samples through gel electrophoresis. Wild type fabF cells show increased activity by expressing ACP, and the mutants show a lack of activity by expressing F-ACP.

challenge
acceptableCORGL:MURC2013-04-12 18:25:42 CDTGO:0008763 UDP-N-acetylmuramate-L-alanine ligase activity (F)PMID:18974047ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows the phosphorylation by MurC by having lower bands on the gel. Figure 4 Shows the essential domains needed for phosphorylation. Figure 6a Shows that MurC has 100% activity at the phosphorylation site.

challenge
updatedbyinstructorCORGL:PKN22013-04-12 18:41:03 CDTGO:0004674 protein serine/threonine kinase activity (F)PMID:18974047ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 3 & 6.

challenge
updatedbyinstructorARATH:KEA22013-04-14 17:12:44 CDTGO:0015386 potassium:hydrogen antiporter activity (F)PMID:22551943ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 8 shows membrane transport after changes in pH.

challenge

acceptable:2
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Messanyn

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
acceptableZstielper,
Team Everything Burrito
HUMAN:IFM2GO:0046597 - negative regulation of viral entry into host cell (P)PMID:23358889ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
updatedbyinstructorMill2034,
Team Its Miller Time
ECOLI:CPXRGO:0045893 - positive regulation of transcription, DNA-templated (P)PMID:15743952ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3
updatedbyinstructorAcoppola,
Team CFP
DROME:Q9NGK5GO:0050805 - negative regulation of synaptic transmission (P)PMID:21228178ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 5
updatedbyinstructorLuispimentelbennaton,
Team CFP
CHICK:NDF1GO:0042479 - positive regulation of eye photoreceptor cell development (P)PMID:9740021ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
requireschangesFkmasuda,
Team The Z-team
DANRE:E7FB98GO:0030656 - regulation of vitamin metabolic process (P)PMID:23533172ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
updatedbyinstructorPungo,
Team The Terminators
CAEEL:AKT1GO:0042771 - intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator (P)PMID:17276923ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2
updatedbyinstructorElkington,
Team Old Worm
CAEEL:G5EGR6GO:0070551 - endoribonuclease activity, cleaving siRNA-paired mRNA (F)PMID:22904066ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
requireschangesTrevornash,
Team Old Worm
?GO:1900368 - regulation of RNA interference (Table 1)PMID:14961122IMP: Inferred from Mutant Phenotype challengeC: 2
updatedbyinstructorZdebstep,
Team Hot Pockets
ECOLX:E2QE90GO:0002949 - tRNA threonylcarbamoyladenosine modification (P)PMID:21183954ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 4

fixed by Zdebstep

0 annotations fixed by Messanyn

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