GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
|Status||Page||Date/Time||GO Term (Aspect)||Reference||Evidence||Notes||Links|
|ECOLI:FABF||2013-04-11 11:38:52 CDT||GO:0033817 beta-ketoacyl-acyl-carrier-protein synthase II activity (F)||PMID:3549687||ECO:0000314 direct assay evidence used in manual assertion|
Figures 1, 2, and 3 show that the fabF mutant (fabF1) activity is decreased by expressing F-ACP. The decrease in activity can be seen by the banding pattern seen after running samples through gel electrophoresis. Wild type fabF cells show increased activity by expressing ACP, and the mutants show a lack of activity by expressing F-ACP.
|CORGL:MURC||2013-04-12 18:25:42 CDT||GO:0008763 UDP-N-acetylmuramate-L-alanine ligase activity (F)||PMID:18974047||ECO:0000314 direct assay evidence used in manual assertion|
Figure 2 shows the phosphorylation by MurC by having lower bands on the gel. Figure 4 Shows the essential domains needed for phosphorylation. Figure 6a Shows that MurC has 100% activity at the phosphorylation site.
|CORGL:PKN2||2013-04-12 18:41:03 CDT||GO:0004674 protein serine/threonine kinase activity (F)||PMID:18974047||ECO:0000315 mutant phenotype evidence used in manual assertion|
Fig 3 & 6.
|ARATH:KEA2||2013-04-14 17:12:44 CDT||GO:0015386 potassium:hydrogen antiporter activity (F)||PMID:22551943||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 8 shows membrane transport after changes in pH.
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