PMID:3549687

Citation	Jackowski, S and Rock, CO (1987) Altered molecular form of acyl carrier protein associated with beta-ketoacyl-acyl carrier protein synthase II (fabF) mutants. J. Bacteriol. 169:1469-73
Abstract	Acyl carrier protein (ACP) is a required cofactor for fatty acid synthesis in Escherichia coli. Mutants lacking beta-ketoacyl-ACP synthase II activity (fabF1 or fabF3) possessed a different molecular species of ACP (F-ACP) that was separated from the normal form of the protein by conformationally sensitive gel electrophoresis. Synthase I mutants contained the normal protein. Complementation of fabF1 mutants with an F' factor harboring the wild-type synthase II allele resulted in the appearance of normal ACP, whereas complementation with an F' possessing the fabF2 allele (a mutation that produces a synthase II enzyme with altered catalytic activity) resulted in the production of both forms of ACP. The structural difference between F-ACP and ACP persisted after the removal of the 4'-phosphopantetheine prosthetic group, and both forms of the protein had identical properties in an in vitro fatty acid synthase assay. Both ACP and F-ACP were purified to homogeneity, and their primary amino acid sequences were determined. The two ACP species were identical but differed from the sequence reported for E. coli E-15 ACP in that an Asn instead of an Asp was at position 24 and an Ile instead of a Val was at position 43. Therefore, F-ACP appears to be a modification of ACP that is detected when beta-ketoacyl-ACP synthase II activity is impaired.
Links	PubMed PMC211969
Keywords	3-Oxoacyl-(Acyl-Carrier-Protein) Synthase/genetics; 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase/metabolism; Acyl Carrier Protein/analysis; Acyl Carrier Protein/isolation & purification; Acyl Carrier Protein/metabolism; Acyltransferases/genetics; Amino Acid Sequence; Escherichia coli/analysis; Escherichia coli/enzymology; Escherichia coli/genetics; Fatty Acids/biosynthesis; Genetic Complementation Test; Mutation; Oleic Acids/biosynthesis
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ECOLI:FABF		GO:0033817: beta-ketoacyl-acyl-carrier-protein synthase II activity	ECO:0000314:		F		Figures 1, 2, and 3 show that the fabF mutant (fabF1) activity is decreased by expressing F-ACP. The decrease in activity can be seen by the banding pattern seen after running samples through gel electrophoresis. Wild type fabF cells show increased activity by expressing ACP, and the mutants show a lack of activity by expressing F-ACP.	complete CACAO 7631
ECOLI:FABF	enables	GO:0033817: beta-ketoacyl-acyl-carrier-protein synthase II activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete

References

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