GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
Category:Team The Knockouts
|Status||Page||User||Date/Time||GO Term (Aspect)||Reference||Evidence||Notes||Links|
|ARATH:KEA2||Messanyn, Team The Knockouts||2013-04-14 17:12:44 CDT||GO:0015386 potassium:hydrogen antiporter activity (F)||PMID:22551943||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 8 shows membrane transport after changes in pH.
|CORGL:PKN2||Messanyn, Team The Knockouts||2013-04-12 18:41:03 CDT||GO:0004674 protein serine/threonine kinase activity (F)||PMID:18974047||ECO:0000315 mutant phenotype evidence used in manual assertion|
Fig 3 & 6.
|CORGL:MURC||Messanyn, Team The Knockouts||2013-04-12 18:25:42 CDT||GO:0008763 UDP-N-acetylmuramate-L-alanine ligase activity (F)||PMID:18974047||ECO:0000314 direct assay evidence used in manual assertion|
Figure 2 shows the phosphorylation by MurC by having lower bands on the gel. Figure 4 Shows the essential domains needed for phosphorylation. Figure 6a Shows that MurC has 100% activity at the phosphorylation site.
|HHV1:Q991M3||Fisch183, Team The Knockouts||2013-04-12 12:12:07 CDT||GO:0010560 positive regulation of glycoprotein biosynthetic process (P)||PMID:23443004||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 5 shows blocking of fusion triggered by gD in cells transfected with gB. Showing that gD decreases the percentages of fusion levels in 52S, LP11, and CHL18.
|PSEAE:DHE2||Fisch183, Team The Knockouts||2013-04-11 12:32:29 CDT||GO:0004352 glutamate dehydrogenase (NAD+) activity (F)||PMID:11133942||ECO:0000314 direct assay evidence used in manual assertion|
Table 2 showed that NAD-GDH was induced 27-fold when PAO1 was grown with arginine as the sole source of carbon and nitrogen relative to the level found when grown with succinate and ammonia as carbon and nitrogen sources, respectively. Proving that the gdhB gene encodes an arginine-inducible NAD+ dependent glutamate dehydrogenase.
|ECOLI:FABF||Messanyn, Team The Knockouts||2013-04-11 11:38:52 CDT||GO:0033817 beta-ketoacyl-acyl-carrier-protein synthase II activity (F)||PMID:3549687||ECO:0000314 direct assay evidence used in manual assertion|
Figures 1, 2, and 3 show that the fabF mutant (fabF1) activity is decreased by expressing F-ACP. The decrease in activity can be seen by the banding pattern seen after running samples through gel electrophoresis. Wild type fabF cells show increased activity by expressing ACP, and the mutants show a lack of activity by expressing F-ACP.