User:Mart1202

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	MYXXA:Q7BU54	2013-03-27 10:32:45 CDT	GO:0000156 phosphorelay response regulator activity (F)	PMID:17581122	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 4B shows that radiolabelled phosphate is transferred from FrzE when in the presence of FrzZ as seen by the appearnace of a radiolabelled band in lane 3 at the FrzZ position. Fig 4B also shows that both domains of FrzZ are able to be phosphorylated by FrzE through the individual mutation of each domain at an aspartate residue, as seen by a decrease in band intensity at the FrzECheA position and the appearance of a radiolabbelled band at the FrzZ position in lanes 4 & 5. Phosphotransfer can not occur when both domains are mutated concurrently at an aspartate residue as evidenced from a lack of a radiolabelled band at the FrzZ position in lane 6.	challenge
acceptable	MYXXA:Q7BU54	2013-03-29 22:52:59 CDT	GO:0071976 cell gliding (P)	PMID:17581122	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig. 3 shows that the strain with mutations in both phosphorylation sites in frzZ exhibits the same phenotype as strains that have a complete deletion of frzZ. As seen in Fig 3B, these two strains have inhibited motility, in comparison to the wild-type strain, as evident by a smaller colony diameter on both 0.3% agar and 1.5% agar. Further, strains with a mutation in one of the possible phosphorylation sites exhibited motility similar to wild-type indicating that phosphorylation can occur at one site or the other as well as the role of frzZ in cell motility.	challenge
updatedbyinstructor	MYXXD:Q1D4V8	2013-04-09 15:26:00 CDT	GO:0071978 bacterial-type flagellar swarming motility (P)	PMID:21856842	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig. 4B illustrates reduced swarm diameter observed in a frzE mutant compared to the swarm diameter observed in WT DK1622. Deletion of this gene does not completely knock out swarming ability but greatly decreases it.	challenge
updatedbyinstructor	MYXXD:Q1D2Y0	2013-04-12 18:26:44 CDT	GO:0048870 cell motility (P)	PMID:22287524	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2 shows that a mutant with a deletion of MXAN_4832 exhibits a similar cell motility phenotype to that of DK1253, an A+S- strain. MXAN_4832 contributes to the regulation of S-motility because deletion of this gene causes the loss of S-motility.	challenge
acceptable	MYXXD:Q1D2Y0	2013-04-12 18:40:58 CDT	GO:0097435 fibril organization (P)	PMID:22287524	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 3A. When the agglutination rate of WT DK1622 was compared to that of Δ4832, the two rates matched one another for the first hour. After 3 hours, the agglutination rate of Δ4832 was reduced compared to that of DK1622. A reduced rate of decrease in agglutination is indicative of a defect in fibril production.	challenge
requireschanges	MYXXD:Q1D2Y0	2013-04-12 20:16:12 CDT	GO:0061077 chaperone-mediated protein folding (P)	PMID:22287524	ECO:0000314 direct assay evidence used in manual assertion	Fig 5 shows the results of a luciferase reactivation assay in which Clp/Hsp100 proteins can prevent the permanent inactivation of luciferase if added prior to heating. When MXAN_4832 was added before the heat shock and KJE is added after the heat shock some activity was restored. No activity was restored to luciferase when MXAN_4832 was absent before the heat shock as well as when KJE was absent after heat shock.	challenge

acceptable:3
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Mart1202

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
updatedbyinstructor	Singhpr4, Team The Monstars	BRASZ:NAAC	GO:0046573 - lactonohydrolase activity (F)	PMID:21498645	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 3 fixed by Mart1202
updatedbyinstructor	Singhpr4, Team The Monstars	PSEAE:PHHC	GO:0006572 - tyrosine catabolic process (P)	PMID:9846749	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1 fixed by Mart1202
updatedbyinstructor	Mart1202, Team Mom Genes	MYXXD:Q1D4V8	GO:0071978 - bacterial-type flagellum-dependent swarming motility (P)	PMID:21856842	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2 fixed by Mart1202
requireschanges	Amanda.karpa, Team Cytoskeleton	MOUSE:ITSN1	GO:0043524 - negative regulation of neuron apoptotic process (P)	PMID:23054079	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 4
updatedbyinstructor	Kulhan11, Team ttKtt	THESS:E8PQ06	GO:0016491 - oxidoreductase activity (F)	PMID:22094050	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1 fixed by Mart1202
updatedbyinstructor	Reddenc1, Team Better Than Hot Pocket	XANC5:Q3BUB5	GO:0043709 - cell adhesion involved in single-species biofilm formation (P)	PMID:21995764	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 6
correctedthroughchallenges	Duquefeg, Team The Monstars	?	GO:0042325 - regulation of phosphorylation (Figure 6 (a)(b)(c)(d) Together suggest that Atr works upstream of Chk1regulating its phosphorylation after UV damage.)	PMID:10859164	IDA: Inferred from Direct Assay	challenge	C: 3 fixed by Mart1202
updatedbyinstructor	Priesko4, Team ttKtt	RHILE:Q20DQ4	GO:0008378 - galactosyltransferase activity (F)	PMID:22110131	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 4 fixed by Herdman1
updatedbyinstructor	Neillmeg, Team Peacock	HUMAN:DCR1A	GO:0035312 - 5'-3' DNA exonuclease activity (F)	PMID:22692201	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 3
updatedbyinstructor	Priesko4, Team ttKtt	LEPIR:C0J1Q3	GO:0045916 - negative regulation of complement activation (P)	PMID:22911815	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1 fixed by Mart1202

6 annotations fixed by Mart1202

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