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PMID:22287524

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Citation

Yan, J, Garza, AG, Bradley, MD and Welch, RD (2012) A Clp/Hsp100 chaperone functions in Myxococcus xanthus sporulation and self-organization. J. Bacteriol. 194:1689-96

Abstract

The Clp/Hsp100 proteins are chaperones that play a role in protein degradation and reactivation. In bacteria, they exhibit a high degree of pleiotropy, affecting both individual and multicellular phenotypes. In this article, we present the first characterization of a Clp/Hsp100 homolog in Myxococcus xanthus (MXAN_4832 gene locus). Deletion of MXAN_4832 causes defects in both swarming and aggregation related to cell motility and the production of fibrils, which are an important component of the extracellular matrix of a swarm. The deletion also affects the formation of myxospores during development, causing them to become sensitive to heat. The protein product of MXAN_4832 can act as a chaperone in vitro, providing biochemical evidence in support of our hypothesis that MXAN_4832 is a functional Clp/Hsp100 homolog. There are a total of 12 Clp/Hsp100 homologs in M. xanthus, including MXAN_4832, and, based on its mutational and biochemical characterization, they may well represent an important group.

Links

PubMed PMC3302451 Online version:10.1128/JB.06492-11

Keywords

Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Endopeptidase Clp/chemistry; Endopeptidase Clp/genetics; Endopeptidase Clp/metabolism; Heat-Shock Proteins/chemistry; Heat-Shock Proteins/genetics; Heat-Shock Proteins/metabolism; Molecular Sequence Data; Myxococcus xanthus/enzymology; Myxococcus xanthus/genetics; Myxococcus xanthus/growth & development; Myxococcus xanthus/metabolism; Sequence Alignment; Spores, Bacterial/enzymology; Spores, Bacterial/genetics; Spores, Bacterial/growth & development; Spores, Bacterial/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MYXXD:Q1D2Y0

involved_in

GO:0097435: supramolecular fiber organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MYXXD:Q1D2Y0

involved_in

GO:0048870: cell motility

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MYXXD:Q1D2Y0

GO:0048870: cell motility

ECO:0000315:

P

Figure 2 shows that a mutant with a deletion of MXAN_4832 exhibits a similar cell motility phenotype to that of DK1253, an A+S- strain. MXAN_4832 contributes to the regulation of S-motility because deletion of this gene causes the loss of S-motility.

complete
CACAO 7747

MYXXD:Q1D2Y0

GO:0097435: fibril organization

ECO:0000315:

P

Fig 3A. When the agglutination rate of WT DK1622 was compared to that of Δ4832, the two rates matched one another for the first hour. After 3 hours, the agglutination rate of Δ4832 was reduced compared to that of DK1622. A reduced rate of decrease in agglutination is indicative of a defect in fibril production.

complete
CACAO 7748

MYXXD:Q1D2Y0

GO:0061077: chaperone-mediated protein folding

ECO:0000314:

P

Fig 5 shows the results of a luciferase reactivation assay in which Clp/Hsp100 proteins can prevent the permanent inactivation of luciferase if added prior to heating. When MXAN_4832 was added before the heat shock and KJE is added after the heat shock some activity was restored. No activity was restored to luciferase when MXAN_4832 was absent before the heat shock as well as when KJE was absent after heat shock.

complete
CACAO 7756


See also

References

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