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User:Trange

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CACAO Spring 2014

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorRAT:DNAS12014-02-02 02:22:28 CSTGO:0004536 deoxyribonuclease activity (F)PMID:8428592ECO:0000314 direct assay evidence used in manual assertion

Fig 4.

challenge
updatedbyinstructorMOUSE:DNAS12014-02-02 02:52:14 CSTGO:0004530 deoxyribonuclease I activity (F)PMID:15015938ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1 has homogenous Dnase1 (+) and homogenous mutant Dnase1 (-) mice. Dnase1 activity was able to be detected in (+) mice in several tissues including stomach, pituitary, kidney, lymph, and thyroid. Double mutants had no Dnase1 activity in any of the tissues.

challenge
unacceptableHUMAN:THBG2014-02-02 03:29:53 CSTGO:0009791 post-embryonic development (P)PMID:22260853ECO:0000314 direct assay evidence used in manual assertion

The second half of Figure 2 shows the progression of thyroxine-binding globulin over time in hypothyroid women from stimulation, to post-embryonic development. It was tested between women who were treated for hypothyroidism vs. women who were not. Overall, the increase in TBG presence is gradual over time, but the increase is not statistically significant.

challenge
unacceptableHUMAN:CLCN12014-02-02 03:58:10 CSTGO:0005254 chloride channel activity (F)PMID:0000037ECO:0000314 direct assay evidence used in manual assertion

Figure 1 depicts an RT-PCR of mRNAs of CLC-1 through CLC-7. Chloride ion flux is a necessary part of corneal wound electric current in human corneal epithelium. Chloride channel proteins 2,3,4, 5, and 6, but chloride channel 1 is not involved.

challenge
unacceptableHUMAN:CLCN12014-02-02 04:11:38 CSTGO:0042493 response to drug (P)PMID:19153558ECO:0000314 direct assay evidence used in manual assertion

Figure 2Ac depicts the increase in chloride sensing of drug by increase in pH. The quick response of chloride ions to drug implies the possibility of chloride channel drug targeting for inhibition of disease progression.

challenge
unacceptableRAT:Q6I9B62014-02-02 17:52:35 CSTGO:0005267 potassium channel activity (F)PMID:17346910ECO:0000314 direct assay evidence used in manual assertion

Figure 3 is a gel electrophoresis of the RT-PCR experiment of amplifying DNA of the rat cochlear nucleus. This gel electrophoresis shows that a subset of potassium channel's are expressed in the rat cochlear nucleus and present in all subdivisions of the cochlear nucleus.

challenge
unacceptableHUMAN:CDKN32014-02-02 18:17:48 CSTGO:0004721 phosphoprotein phosphatase activity (F)PMID:18669648ECO:0000314 direct assay evidence used in manual assertion

Figure 5 B and C are entire proteome topographical plots of up-regulated phosphorylation in G1 (B) and M-phase (C) cells. M-phase cells have much more M-phase phosphoprotein phosphatase activity than G1 protein activity seen in G1 cells based on a Protein Regulated Phosphorylation (P-RePh) scores > 10.

challenge

acceptable:0
unacceptable:5
requires_changes:0
flagged:0

Annotations challenged by Trange

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorMalexander2,
Team Pancakes At Nite
HUMAN:CIB1GO:0005886 - plasma membrane (C)PMID:20473878ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3

fixed by Grevever
updatedbyinstructorMalexander2,
Team Pancakes At Nite
HUMAN:CIB1GO:0030291 - protein serine/threonine kinase inhibitor activity (F)PMID:20473878ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
updatedbyinstructorTrange,
Team Kitten Me Right Meow
RAT:DNAS1GO:0004536 - deoxyribonuclease activity (F)PMID:8428592ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3
updatedbyinstructorKdiawara,
Team Lobster Soul
HUMAN:HS90BGO:0097435 - supramolecular fiber organization (P)PMID:24466266ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 8
unacceptableRthompson,
Team Bfast Taco
HUMAN:PEPA5GO:0006508 - proteolysis (P)other:PMC1712889ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3
unacceptableKfree,
Team Bfast Taco
MOUSE:ACHA7GO:0050728 - negative regulation of inflammatory response (P)PMID:24454942ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1

0 annotations fixed by Trange

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