User:Kdiawara

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	HUMAN:HS90B	2014-01-30 16:13:07 CST	GO:0097435 fibril organization (P)	PMID:24466266	ECO:0000315 mutant phenotype evidence used in manual assertion	An Interaction observed in vitro and colocalization of Hsp90 and FN in both normal and cancer cell lines were investigated using confocal microscopy and the DOC assay. These methods helped to determine the effect of exogenous Hsp90β on FN matrix formation and morphology in Hs578T cells. Treatment of the Hs578T cell line with exogenous soluble Hsp90β led to an increase in insoluble FN relative to soluble FN, suggesting a role for extracellular Hsp90 in fibril formation. Other methods used include co-immunoprecipitation, solid phase binding assay, immunoblot analysis, SPR spectroscopy and mass spectrometry.	challenge
unacceptable	9HIV1:Q2ME96	2014-01-30 16:15:34 CST	GO:2000353 positive regulation of endothelial cell apoptotic process (P)	PMID:24318111	ECO:0000315 mutant phenotype evidence used in manual assertion	Results show a series of graphs and fgures to portray that concentrations of gp120 enhanced the induction of apoptosis in cigarette smoke exposed endothelial cells; Gel Electrophoresis was used.	challenge
updatedbyinstructor	SULSO:CAS4	2014-01-30 16:31:18 CST	GO:0045145 single-stranded DNA 5'-3' exodeoxyribonuclease activity (F)	PMID:23056615	ECO:0000314 direct assay evidence used in manual assertion	Figures 4,5- the exonuclease activity of the gene was studied. A DNA oligonucleotide substrate was labeled at the 5' and 3' terminus with fluorescence. When labeled at the 5' end, the product had a size of around 4 nucleotides, while the 3' end had products that got progressively smaller until they reached 1-2 nucleotides in length. This shows that the gene is involved in the 5' to 3' exodeoxyribonuclease activity and initiates cleavage at the 5' end.	challenge
unacceptable	HUMAN:CH60	2014-01-30 17:48:09 CST	GO:0046789 host cell surface receptor binding (F)	PMID:12496435	ECO:0000314 direct assay evidence used in manual assertion	2D gel electrophoresis, flow cytometric analysis and other methods were used and analyzed to determine and identify the ligand(s) on the surface of Hc yeasts that binds to human macrophage CD11/CD18.	challenge

acceptable:0
unacceptable:2
requires_changes:0
flagged:0

Annotations challenged by Kdiawara

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
updatedbyinstructor	Kdiawara, Team Lobster Soul	HUMAN:HS90B	GO:0097435 - supramolecular fiber organization (P)	PMID:24466266	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 8
unacceptable	Kdiawara, Team Lobster Soul	HUMAN:CH60	GO:0046789 - host cell surface receptor binding (F)	PMID:12496435	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 4

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