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CACAO Spring 2014

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorHUMAN:HS90B2014-01-30 16:13:07 CSTGO:0097435 fibril organization (P)PMID:24466266ECO:0000315 mutant phenotype evidence used in manual assertion

An Interaction observed in vitro and colocalization of Hsp90 and FN in both normal and cancer cell lines were investigated using confocal microscopy and the DOC assay. These methods helped to determine the effect of exogenous Hsp90β on FN matrix formation and morphology in Hs578T cells. Treatment of the Hs578T cell line with exogenous soluble Hsp90β led to an increase in insoluble FN relative to soluble FN, suggesting a role for extracellular Hsp90 in fibril formation. Other methods used include co-immunoprecipitation, solid phase binding assay, immunoblot analysis, SPR spectroscopy and mass spectrometry.

unacceptable9HIV1:Q2ME962014-01-30 16:15:34 CSTGO:2000353 positive regulation of endothelial cell apoptotic process (P)PMID:24318111ECO:0000315 mutant phenotype evidence used in manual assertion

Results show a series of graphs and fgures to portray that concentrations of gp120 enhanced the induction of apoptosis in cigarette smoke exposed endothelial cells; Gel Electrophoresis was used.

updatedbyinstructorSULSO:CAS42014-01-30 16:31:18 CSTGO:0045145 single-stranded DNA 5'-3' exodeoxyribonuclease activity (F)PMID:23056615ECO:0000314 direct assay evidence used in manual assertion

Figures 4,5- the exonuclease activity of the gene was studied. A DNA oligonucleotide substrate was labeled at the 5' and 3' terminus with fluorescence. When labeled at the 5' end, the product had a size of around 4 nucleotides, while the 3' end had products that got progressively smaller until they reached 1-2 nucleotides in length. This shows that the gene is involved in the 5' to 3' exodeoxyribonuclease activity and initiates cleavage at the 5' end.

unacceptableHUMAN:CH602014-01-30 17:48:09 CSTGO:0046789 host cell surface receptor binding (F)PMID:12496435ECO:0000314 direct assay evidence used in manual assertion

2D gel electrophoresis, flow cytometric analysis and other methods were used and analyzed to determine and identify the ligand(s) on the surface of Hc yeasts that binds to human macrophage CD11/CD18.



Annotations challenged by Kdiawara

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
Team Lobster Soul
HUMAN:HS90BGO:0097435 - supramolecular fiber organization (P)PMID:24466266ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 8
Team Lobster Soul
HUMAN:CH60GO:0046789 - host cell surface receptor binding (F)PMID:12496435ECO:0000314 direct assay evidence used in manual assertionchallengeC: 4

0 annotations fixed by Kdiawara

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