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Category:Team Lobster Soul

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorCHICK:MLE1Csobelman, Team Lobster Soul2014-02-02 14:08:02 CSTGO:0043520 regulation of myosin II filament assembly (P)PMID:1464306ECO:0000314 direct assay evidence used in manual assertion

Fig 3

unacceptableCHICK:MLE1Csobelman, Team Lobster Soul2014-02-02 13:47:42 CSTGO:0006936 muscle contraction (P)PMID:6238848ECO:0000314 direct assay evidence used in manual assertion

Figure 2 A shows the sub fragments of myosin chains that helps in movement. The recent crystal structure of myosin subfragment-1 suggests the presence of two distinct domains: a catalytic or motor domain, from which extends a long, 8.5-nm alpha-helix that is stabilized by the regulatory and essential light chains.

unacceptableMOUSE:P53Csobelman, Team Lobster Soul2014-02-02 12:16:26 CSTGO:0042771 intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator (P)PMID:12952892ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1 A shows DNA damage-induced apoptosis of E1A-expressing MEFs. MEFs were infected with control (pBabe) or E1A-expressing retrovirus (pBabe-E1A), and were treated with adriamycin (0.25 μg/mL) for 24 h. Wild-type (WT) and Noxa-/- MEFs (left), as well as Noxa-/- and Noxa-/-/Bax-/- MEFs (right), were each prepared from the same litter. Results are representative of three independent experiments.

unacceptableMOUSE:FOXP2Csobelman, Team Lobster Soul2014-02-02 11:20:39 CSTGO:0042297 vocal learning (P)PMID:15983371ECO:0000315 mutant phenotype evidence used in manual assertion

Disruption of both copies of the Foxp2 gene caused severe motor impairment, premature death, and an absence of ultrasonic vocalizations that are elicited when pups are removed from their mothers. Figure 1b shows a southern blot analysis showing the alteration of the mutant foxp2 allele.

unacceptableHUMAN:CH60Kdiawara, Team Lobster Soul2014-01-30 17:48:09 CSTGO:0046789 host cell surface receptor binding (F)PMID:12496435ECO:0000314 direct assay evidence used in manual assertion

2D gel electrophoresis, flow cytometric analysis and other methods were used and analyzed to determine and identify the ligand(s) on the surface of Hc yeasts that binds to human macrophage CD11/CD18.

updatedbyinstructorSULSO:CAS4Kdiawara, Team Lobster Soul2014-01-30 16:31:18 CSTGO:0045145 single-stranded DNA 5'-3' exodeoxyribonuclease activity (F)PMID:23056615ECO:0000314 direct assay evidence used in manual assertion

Figures 4,5- the exonuclease activity of the gene was studied. A DNA oligonucleotide substrate was labeled at the 5' and 3' terminus with fluorescence. When labeled at the 5' end, the product had a size of around 4 nucleotides, while the 3' end had products that got progressively smaller until they reached 1-2 nucleotides in length. This shows that the gene is involved in the 5' to 3' exodeoxyribonuclease activity and initiates cleavage at the 5' end.

unacceptable9HIV1:Q2ME96Kdiawara, Team Lobster Soul2014-01-30 16:15:34 CSTGO:2000353 positive regulation of endothelial cell apoptotic process (P)PMID:24318111ECO:0000315 mutant phenotype evidence used in manual assertion

Results show a series of graphs and fgures to portray that concentrations of gp120 enhanced the induction of apoptosis in cigarette smoke exposed endothelial cells; Gel Electrophoresis was used.

updatedbyinstructorHUMAN:HS90BKdiawara, Team Lobster Soul2014-01-30 16:13:07 CSTGO:0097435 fibril organization (P)PMID:24466266ECO:0000315 mutant phenotype evidence used in manual assertion

An Interaction observed in vitro and colocalization of Hsp90 and FN in both normal and cancer cell lines were investigated using confocal microscopy and the DOC assay. These methods helped to determine the effect of exogenous Hsp90β on FN matrix formation and morphology in Hs578T cells. Treatment of the Hs578T cell line with exogenous soluble Hsp90β led to an increase in insoluble FN relative to soluble FN, suggesting a role for extracellular Hsp90 in fibril formation. Other methods used include co-immunoprecipitation, solid phase binding assay, immunoblot analysis, SPR spectroscopy and mass spectrometry.

unacceptableHUMAN:P53Csobelman, Team Lobster Soul2014-01-29 20:40:04 CSTGO:0000075 cell cycle checkpoint (P)Reactome:REACT_1538ECO:0000314 direct assay evidence used in manual assertion

Mutations in the p53 gene have been associated with human tumors including osteosarcomas. This study shows that the over expression of the gene encoding wild-type p53 blocks the growth of osteosarcoma cells. The growth arrest was determined to be due to an inability of the transfected cells to progress to S phase. This suggests that the told of p53 gene as an antioncogene may be in controlling the cell cycle in a fashion analogous to the check point control genes in Saccharomyces cerevisiae. Immunofluorescence and Southern blots were used.


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Pages in category "Team Lobster Soul"

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