User:Csobelman

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	HUMAN:P53	2014-01-29 20:40:04 CST	GO:0000075 cell cycle checkpoint (P)	Reactome:REACT_1538	ECO:0000314 direct assay evidence used in manual assertion	Mutations in the p53 gene have been associated with human tumors including osteosarcomas. This study shows that the over expression of the gene encoding wild-type p53 blocks the growth of osteosarcoma cells. The growth arrest was determined to be due to an inability of the transfected cells to progress to S phase. This suggests that the told of p53 gene as an antioncogene may be in controlling the cell cycle in a fashion analogous to the check point control genes in Saccharomyces cerevisiae. Immunofluorescence and Southern blots were used.	challenge
unacceptable	MOUSE:FOXP2	2014-02-02 11:20:39 CST	GO:0042297 vocal learning (P)	PMID:15983371	ECO:0000315 mutant phenotype evidence used in manual assertion	Disruption of both copies of the Foxp2 gene caused severe motor impairment, premature death, and an absence of ultrasonic vocalizations that are elicited when pups are removed from their mothers. Figure 1b shows a southern blot analysis showing the alteration of the mutant foxp2 allele.	challenge
unacceptable	MOUSE:P53	2014-02-02 12:16:26 CST	GO:0042771 intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator (P)	PMID:12952892	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1 A shows DNA damage-induced apoptosis of E1A-expressing MEFs. MEFs were infected with control (pBabe) or E1A-expressing retrovirus (pBabe-E1A), and were treated with adriamycin (0.25 μg/mL) for 24 h. Wild-type (WT) and Noxa-/- MEFs (left), as well as Noxa-/- and Noxa-/-/Bax-/- MEFs (right), were each prepared from the same litter. Results are representative of three independent experiments.	challenge
unacceptable	CHICK:MLE1	2014-02-02 13:47:42 CST	GO:0006936 muscle contraction (P)	PMID:6238848	ECO:0000314 direct assay evidence used in manual assertion	Figure 2 A shows the sub fragments of myosin chains that helps in movement. The recent crystal structure of myosin subfragment-1 suggests the presence of two distinct domains: a catalytic or motor domain, from which extends a long, 8.5-nm alpha-helix that is stabilized by the regulatory and essential light chains.	challenge
updatedbyinstructor	CHICK:MLE1	2014-02-02 14:08:02 CST	GO:0043520 regulation of myosin II filament assembly (P)	PMID:1464306	ECO:0000314 direct assay evidence used in manual assertion	Fig 3	challenge

acceptable:0
unacceptable:4
requires_changes:0
flagged:0

Annotations challenged by Csobelman

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	Relledge, Team Blot Blot Wstrn Baby	MOUSE:TNR16	GO:0045773 - positive regulation of axon extension (P)	PMID:3815101	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 3
unacceptable	Tatkinson, Team Thndrcats Ho!	HUMAN:FLOT1	GO:0071806 - protein transmembrane transport (P)	PMID:24465508	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 3
updatedbyinstructor	Kfree, Team Bfast Taco	MOUSE:ACHB4	GO:0051971 - positive regulation of transmission of nerve impulse (P)	PMID:12606764	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 5 fixed by Kfree
unacceptable	Rthompson, Team Bfast Taco	HUMAN:OPSD	GO:0071482 - cellular response to light stimulus (P)	PMID:8643442	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 4

0 annotations fixed by Csobelman

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