GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
Cacao
| GO:0045145 | single-stranded DNA 5'-3' exodeoxyribonuclease activity | PMID:23056615 | IDA: Inferred from Direct Assay | F | ||||
| This annotation made on page: SULSO:CAS4 By: Kdiawara (group Team Lobster Soul) on 2014-01-30 16:31:18 CST. | ||||||||
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| Entry Type | Challenging User,Group | Time/Date | Challenge Reason | Points/Assessment |
|---|---|---|---|---|
| Challenge | Jack1034, MichSt14A 38 | 2014-04-12 21:39:26 CDT | In Figure 4, the exonuclease activity of the gene was studied. A DNA oligonucleotide substrate was labeled at the 5' and 3' terminus with fluorescence. When labeled at the 5' end, the product had a size of around 4 nucleotides, while the 3' end had products that got progressively smaller until they reached 1-2 nucleotides in length. This shows that the gene is involved in the 5' to 3' exodeoxyribonuclease activity and initiates cleavage at the 5' end. | 5 |
| Challenge | Sirons, Team Takin This Outside | 2014-02-06 22:28:44 CST | invalid pubmed ID | 0 |
| Private Assessment | Suzialeksander | 2014-05-09 12:09:14 CDT | You need to be an instructor to view these notes. | Updated by Instructor |
| Private Assessment | JimHu | 2014-04-19 19:44:22 CDT | You need to be an instructor to view these notes. | Corrected Through Challenges ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✔ Notes ✔ Unique/Original |
| Public Assessment | DanielRenfro | 2014-04-12 21:39:26 CDT | This annotation has been flagged because it has been edited since last assessment ||GO:0045145||single-stranded DNA 5'-3' exodeoxyribonuclease activity||PMID:23056615||IDA: Inferred from Direct Assay||||F||It has been demonstrated through methods such as gel Electrophoresis, gel filtration chromatography, and Iron chelation assay that the Cas4 family of proteins, associated with obtaining new spacer sequences in the CRISPR system, are 5′ end directed 5′–3′ ssDNA exonucleases with an iron-sulfur cluster binding site. ||complete | Flagged |
| Private Assessment | Suzialeksander | 2014-02-17 14:39:01 CST | You need to be an instructor to view these notes. | Requires Changes ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✗ Notes ✔ Unique/Original |
