| unacceptable | 9CAUD:J9PVF5 | 2016-04-25 10:02:39 CDT | GO:0016301 kinase activity (F) | GO_REF:0000100 | ECO:0000250 sequence similarity evidence used in manual assertion | On Blast, this protein matches the Dephospho-CoA Kinase protein from Bacillus phage Spock with a 100% coverage, 82% identity, and an e-value of 3e-113. On HHpred, the probability of this gene being Dephospho-CoA Kinase is 99.8%, and the e-value is 1.1e-18. Also, in the article, Table 2 shows functional grouping of proteins, which includes the dephospho-CoA kinase, however not much more information is provided in the article.
| challenge |
| unacceptable | 9CAUD:A0A0K2FLF8 | 2016-04-20 09:35:51 CDT | GO:0016301 kinase activity (F) | GO_REF:0000100 | ECO:0000250 sequence similarity evidence used in manual assertion | On Blast, the protein matches the Dephospho-CoA Kinase protein in Bacillus phage Spock with an 81% coverage, 39% identity, and an e-value of 8e-33. On HHpred, the probability of this gene being Dephospho-CoA Kinase is 99.8%, and the e-value is 4.4e-18.
| challenge |
| unacceptable | 9CAUD:S5Y0G8 | 2016-04-11 09:59:26 CDT | GO:0016301 kinase activity (F) | GO_REF:0000100 | ECO:0000250 sequence similarity evidence used in manual assertion | On Blast, the gene matched the Dephospho-CoA Kinase on Bacillus phage Riley with 100% coverage, 99% identity, and an e-value of 2e-141. On HHpred, there is a 99.8% probability that this gene is, in fact, Dephospho-CoA Kinase. The e-value on HHpred is 1.1e-17.
| challenge |
| unacceptable | 9CAUD:J9PLX2 | 2016-04-20 09:38:48 CDT | GO:0016301 kinase activity (F) | GO_REF:0000100 | ECO:0000250 sequence similarity evidence used in manual assertion | On Blast, this gene from Bastille matched with the Adenylate Kinase gene from Bacillus phage Evoli with a 100% coverage, 89% identity, and an e-value of 1e-123. Also, HHpred says that there is a 99.9% probability that Adenylate Kinase is the gene's function. The e-value from HHpred is 1.7e-20.
| challenge |
| unacceptable | 9CAUD:Q8H9R7 | 2016-04-19 09:05:36 CDT | GO:0098009 virus terminase, large subunit (C) | PMID:23011306 | ECO:0000314 direct assay evidence used in manual assertion | The Orf3 Gene has a terminase function. Terminase typically has two subunits, the smaller is responsible for binding the viral DNA, while the larger subunit is responsible primarily for DNA cleavage and translocation. This particular gene makes the protein p03, which is the larger subunit in the terminase process (Figure 1). When unassembled from p01, p03 can typically perform the binding function by itself, however, when performing endonucleolytic activity, efficiency was tested, and was found to perform higher when both p01 and p03 were present (Figure 4).
| challenge |
| unacceptable | 9CAUD:K4F7V6 | 2016-04-20 09:39:35 CDT | GO:0098009 virus terminase, large subunit (For gene GAP52_005, the Blastp results, when compared with phage PaP3, showed 93% coverage, 42% identity, and an e-value of 4e-110 in favor of the large subunit of terminase. When run through HHpred, it gave a 99.9% probability with an e-vaule of 2.9e-23 for the large subunit of a terminase protein.) | GO_REF:0000100 | ISS: Inferred from Sequence Similarity UniProtKB:K4F7V6
UniProtKB:Q8H9R7
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| updatedbyinstructor | 9CAUD:A0A0B5A471 | 2016-04-20 09:40:18 CDT | GO:0043493 viral terminase complex (C) | GO_REF:0000100 | ECO:0000250 sequence similarity evidence used in manual assertion | The Blastp results for gene PhiCHU_71, when compared with phage PaP3, showed 100% coverage, 99.8% identity, and an e-value of 0.0 (the e-value gets rounded when it is close enough to 0) in favor of the large subunit of terminase. When run through HHpred, it gave a 99.9% probability with an e-vaule of 1.5e-23 for the large subunit of terminase protein.
| challenge |
| updatedbyinstructor | 9CAUD:A0A0F6SJ84 | 2016-04-20 09:40:53 CDT | GO:0043493 viral terminase complex (C) | GO_REF:0000100 | ECO:0000250 sequence similarity evidence used in manual assertion | When run through Blastp, this gene matched with the orf3 gene from the phage PaP3 (terminase large subunit) with a 100% coverage, 97% identity, and an e-value of 0.0 (when the e-value gets too low, Blast automatically rounds to 0.0). On HHpred, there is a 99.9% probability that the gene is the large subunit of terminase, and an e-value of 1.5e-23.
| challenge |
| updatedbyinstructor | 9CAUD:W0XAC1 | 2016-04-20 09:41:26 CDT | GO:0043493 viral terminase complex (C) | GO_REF:0000100 | ECO:0000250 sequence similarity evidence used in manual assertion | The Blastp results for this gene showed a 97% identity, 100% coverage, and an e-value of 0.0 (Blast automatically rounds down when the e-value is very small) match to the large subunit of terminase gene in phage PaP3. HHpred showed a 99.9% probability and an e-value of 3e-23 for the large subunit in terminase function.
| challenge |
| updatedbyinstructor | 9CAUD:K0ILM7 | 2016-04-25 10:13:30 CDT | GO:0043493 viral terminase complex (C) | GO_REF:0000100 | ECO:0000247 sequence alignment evidence used in manual assertion | On Blastp, this protein matched up with the terminase, large subunit protein of Pseudomonas phage PaP3 with a 100% coverage match, and an 81% identity match. The e-value came back as 0.0 (the e-values get rounded when they're too small).
| challenge |
| updatedbyinstructor | 9CAUD:Q8H9R7 | 2016-04-27 09:39:39 CDT | GO:0016887 ATPase activity (F) | PMID:23011306 | ECO:0000314 direct assay evidence used in manual assertion | ATPase Activity has been found to be largely associated with the large subunit in terminase in this phage. This has been found through sequence analysis, as well as experimentally. The ATPase activity from p03 gel filtration columns were analyzed and it was found that there is a direct, positive correlation between the amount of product made and the presence of p03. The ATPase product greatly increases with the addition of p01 (the small subunit of terminase) as well. See Figure 6.
| challenge |
| updatedbyinstructor | 9CAUD:Q8H9R7 | 2016-04-27 09:53:59 CDT | GO:0043493 viral terminase complex (C) | PMID:23011306 | ECO:0000314 direct assay evidence used in manual assertion | The terminase function in this phage requires both of the proteins p01 and p03. The p01 protein in this phage corresponds to the small subunit of terminase, while p03 corresponds to the large subunit of terminase. Although p03 can function without p01, p01 cannot function without p03. However, the endonucleolytic activity of p03 functions more effectively with the presence of p01. Thus exhibiting a cos-dependent endonucleolytic activity. See Figure 3
| challenge |
| unacceptable | BPT4:PORTL | 2016-04-27 10:34:45 CDT | GO:0046718 viral entry into host cell (P) | PMID:24126213 | ECO:0000314 direct assay evidence used in manual assertion | This protein aids in head assembly, genome packaging, and genome injection. The most common function is of a portal protein that aids in infection by forming a channel, connecting the phage to the membrane of the host bacteria. See Figure 5 for complete outline and analysis of function.
| challenge |
