User:Eleo1

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	BPTU2:Q9AYV6	2016-03-28 05:56:41 CDT	GO:0098023 virus tail, tip (C)	PMID:15150235	ECO:0000314 direct assay evidence used in manual assertion	figure 6 shows gene product specific antibody binding to the tip of the phage tail	challenge
updatedbyinstructor	BPT7:ENDO	2016-04-11 10:55:11 CDT	GO:1990238 double-stranded DNA endodeoxyribonuclease activity (F)	PMID:5263754	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1 shows significant degradation of both double and single stranded DNA upon infection with T7. The researchers, therefore, mutated a number of genes, and found out that only the mutants in genes 1 or 3 did not induce an increased endonuclease activity. It is, furthermore, stated that the product of gene 1 (RNA polymerase factor) is needed for the transcription of most of the T7 genome. Mutants in gene 3 were still synthesizing all essential T7 proteins, only lacking the increased endonuclease activity.	challenge
updatedbyinstructor	BPT7:ENDO	2016-04-11 10:55:11 CDT	GO:1990238 double-stranded DNA endodeoxyribonuclease activity (F)	PMID:5541763	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1 shows endonuclease activity after T7 infection of E. coli B. The endonuclease activity was not seen after infection with a mutant in gene 3 (Table 1).	challenge
updatedbyinstructor	BPT7:ENDO	2016-04-11 10:55:11 CDT	GO:1990238 double-stranded DNA endodeoxyribonuclease activity (F)	PMID:5541763	ECO:0000314 direct assay evidence used in manual assertion	The enzyme was purified and tested against various sources DNA. Breaks in the DNA strands were found (3-4 breaks per single strand before double strand breakage (see Figure 3).	challenge
updatedbyinstructor	BPT7:ENDO	2016-04-11 10:55:12 CDT	GO:0008821 crossover junction endodeoxyribonuclease activity (F)	PMID:9236119	ECO:0000314 direct assay evidence used in manual assertion	After binding to the DNA junction as a dimer, the two subunits of the T7 endonuclease 1 catalyze cleavages independently but rather simultaneously before either is released from the junction (within the lifetime of the protein-junction complex) (Figure 6 and text around it, and Discussion). Similar characteristics have been found in T4 endonuclease VII.	challenge

acceptable:1
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Eleo1

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	Jermstadsb, Team TRUMP2016	BPT4:TERL	GO:0016887 - ATP hydrolysis activity (F)	PMID:19109896	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 5
unacceptable	Gerbermm, Team Phiesty Phages	9CAUD:A0A024B1G2	GO:0008301 - DNA binding, bending (F)	PMID:26673721	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2
acceptable	Eleo1, Team Red B	BPTU2:Q9AYV6	GO:0098023 - virus tail, tip (C)	PMID:15150235	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 4

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