BPT4:TERL

Species (Taxon ID)	Enterobacteria phage T4 (Bacteriophage T4). (10665)
Gene Name(s)	17
Protein Name(s)	Terminase, large subunit DNA-packaging protein Gp17 Gene product 17 gp17 Terminase large subunit
External Links
UniProt	P17312
EMBL	X52394 AF158101 AF158101 AF158101 AF158101
PIR	JU0287
RefSeq	NP_049776.1 NP_049777.1 NP_049778.1 NP_049779.1
PDB	2O0H 2O0J 2O0K 3CPE 3EZK
PDBsum	2O0H 2O0J 2O0K 3CPE 3EZK
ProteinModelPortal	P17312
SMR	P17312
DIP	DIP-60322N
GeneID	1258545 1258647 1258656 1258675
EvolutionaryTrace	P17312
Proteomes	UP000009087
GO	GO:0005524 GO:0004518 GO:0046797 GO:0019076
InterPro	IPR027417 IPR004921
Pfam	PF03237
SUPFAM	SSF52540

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0046797	viral procapsid maturation	PMID:660719	ECO:0000314			P		Figure 2	complete CACAO 7040
	GO:0016887	ATPase activity	PMID:19109896	ECO:0000315			F		The ATPase protein was compared to two mutants (W533A and ΔS527G538) which had weakened interactions between their domains. The mutants were then compared to the wild-type with a Packaging ATPase assay. Both of the mutants had a loss of their ATPase activity. This is proven in figure 3e, where the concentration of cold ATP was increased every hour, for the wild-type and mutants. The third phosphate group was only cleaved in only the wild-type samples, which is seen in the appearance of the upper bands in the thin layer chromotherapy.	complete CACAO 11500
Contributes to	GO:0043493	viral terminase complex	PMID:3294420	ECO:0000314			C		gp16 and gp17 Terminase genes from T4 were over expressed in Ecoli bacteria in the presence of empty phage proheads in order to asses the headful DNA packaging abilities of the genes. Gp16 and gp17 were expressed individually and together in a complex. It was found that, "the gp16 function of the terminase complex is dispensable for packaging mature DNA, whereas gp17 is essential for packaging DNA under any condition tested." It was therefore determined that gp17 is the main Terminase protein in T4 necessary for the molecular function of DNA packaging.	complete CACAO 11547
	GO:0098009	virus terminase, large subunit	PMID:19109896	ECO:0000314			C		Fig 1 of the paper illustrates gp17 as a large terminase.	complete CACAO 11608
	GO:0004518	nuclease activity	PMID:19109896	ECO:0000314			F		The assay experiment of the gp17 terminase can be viewed in figure 3b. Lane's 0, 1, and 2 are how many hours after the addition of isopropyl β-D-1-thiogalactopyranoside (IPTG). This induces the expression of the DNA samples. The mutant sample are then compared to the known DNA. It is then compared to the mutant types, the mutant types did not have cleaved DNA. Proving its identity as a nuclease.	complete CACAO 11955
	GO:0043493	viral terminase complex		ECO:0000255			C		Terminate acts as a an ATP driven molecular motor for viral DNA translocation	Missing: with/from, reference CACAO 12012
	GO:0004536	deoxyribonuclease activity	PMID:19109896	ECO:0000315			F		Figure 3b compares the mutant gp17 proteins to the wild types. Arg406 in T4 participates in binding DNA and nuclease activity. Mutant "Arg406Ala" showed that any substitutions in amino acids at the 406 position produced a null phenotype. Meaning that when they tested the phages in a plating assay the mutants failed to produce plaques. See figure 3b shows that unlike the wild-type, the mutant R406A did not degrade the DNA after 2 hours of incubation.	complete CACAO 12014
involved_in	GO:0019072	viral genome packaging	PMID:18627466	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
enables	GO:0004518	nuclease activity	PMID:18627466	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
part_of	GO:0098009	viral terminase, large subunit	PMID:18627466	ECO:0000314	direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
involved_in	GO:0046797	viral procapsid maturation	PMID:660719	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0090305	nucleic acid phosphodiester bond hydrolysis	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0004518	P		Seeded From UniProt	complete
involved_in	GO:0090305	nucleic acid phosphodiester bond hydrolysis	GO_REF:0000108	ECO:0000366	evidence based on logical inference from automatic annotation used in automatic assertion	GO:0004518	P		Seeded From UniProt	complete
enables	GO:0016787	hydrolase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0378	F		Seeded From UniProt	complete
enables	GO:0046872	metal ion binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0479	F		Seeded From UniProt	complete
enables	GO:0000166	nucleotide binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0547	F		Seeded From UniProt	complete
involved_in	GO:0032359	provirus excision	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1250	P		Seeded From UniProt	complete
enables	GO:0004518	nuclease activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0540	F		Seeded From UniProt	complete
enables	GO:0005524	ATP binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0067	F		Seeded From UniProt	complete
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References

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