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GO:0034235GPI anchor bindingother:http://www.ncbi.nlm.nih.gov/pubmed/?term=19109896IDA: Inferred from Direct Assay F
This annotation made on page: BPT4:TERL
By: Jermstadsb (group Team TRUMP2016) on 2016-04-05 07:32:33 CDT.



TeamPoints
Team TRUMP20165




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeEleo1,
Team Red B
2016-04-18 14:46:40 CDT

Oh and validation of the GO terms I suggested earlier can be found in Figure 3 and in the text below it.

None, yet.
ChallengeEleo1,
Team Red B
2016-04-18 14:44:56 CDT

In agreement with the challenge by connor.long a different GO term should be used. As a matter of fact, CACAO students are not allowed to use 'binding' terms. You could use terms like these: GO:0006323 DNA packaging GO:0016887 ATPase activity

As for the PubMed ID: you can find that on the ncbi website following the link you already provided. (PMID: 19109896)

None, yet.
ChallengeSpanch1,
Team Red B
2016-04-18 09:03:02 CDT

use the pubmed ID!

None, yet.
ChallengeConnor.Long,
Team Tiger1
2016-04-12 14:19:56 CDT

Multiple GO terms are possible for this paper as the "energy for compacting DNA" (N-terminal ATPase domain), "terminat[ion] [of] packaging" (C-terminal nuclease domain), and "translocat[ion] [of] the genome into the procapsid" (C-terminal domain) are all discussed in the abstract specifically and the discussion portion of the paper (quotes were taking from abstract).

However, GPI anchor binding, in my opinion, would not be an appropriate term for this specific paper as the main focus centers around the the stimulation and termination of viral DNA packaging as well as the movement of the genome into the procapsid.

More appropriate GO terms would be:

- Viral DNA genome packaging
- DNA packaging complex
- Viral genome packaging

Furthermore PMID is 19109896

- challenging reference as well because there is a usable PMID code
None, yet.
ChallengeE63,
Team Blue A
2016-04-11 18:24:30 CDT

How can you assume that the structure created from the model functions as a GPI anchor for binding?

Just something minor - the PMID is: 19109896

None, yet.
Public
Assessment
AAJohnson2016-05-16 08:39:34 CDT

The notes accurately describe an experiment showing ATPase activity for the appropriate uniprot ID.

Acceptable
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2016-05-02 10:47:33 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0016887 ATPase activity PMID:19109896 IMP: Inferred from Mutant Phenotype F The ATPase protein was compared to two mutants (W533A and ΔS527G538) which had weakened interactions between their domains. The mutants were then compared to the wild-type with a Packaging ATPase assay. Both of the mutants had a loss of their ATPase activity. This is proven in figure 3e, where the concentration of cold ATP was increased every hour, for the wild-type and mutants. The third phosphate group was only cleaved in only the wild-type samples, which is seen in the appearance of the upper bands in the thin layer chromotherapy. complete
CACAO 11500
on BPT4:TERL
Flagged