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PMID:5541763
| Citation |
Sadowski, PD (1971) Bacteriophage T7 endonuclease. I. Properties of the enzyme purified from T7 phage-infected Escherichia coli B. J. Biol. Chem. 246:209-16 |
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| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Centrifugation, Density Gradient; Chloromercuribenzoates; Coliphages/enzymology; DNA, Bacterial; Deoxyribonucleases/antagonists & inhibitors; Deoxyribonucleases/isolation & purification |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:1990238: double-stranded DNA endodeoxyribonuclease activity |
ECO:0000315: |
F |
Figure 1 shows endonuclease activity after T7 infection of E. coli B. The endonuclease activity was not seen after infection with a mutant in gene 3 (Table 1). |
complete | ||||
| GO:1990238: double-stranded DNA endodeoxyribonuclease activity |
ECO:0000314: |
F |
The enzyme was purified and tested against various sources DNA. Breaks in the DNA strands were found (3-4 breaks per single strand before double strand breakage (see Figure 3). |
complete | ||||
Notes
See also
References
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