BPT7:ENDO

Species (Taxon ID)	Enterobacteria phage T7 (Bacteriophage T7). (10760)
Gene Name(s)	No Information Provided.
Protein Name(s)	Endonuclease I Gene product 3 Gp3 Junction-resolving enzyme gp3
External Links
UniProt	P00641
EMBL	V01127 V01146
PIR	B94615
RefSeq	NP_041972.1
PDB	1FZR 1M0D 1M0I 2PFJ 3CAE
PDBsum	1FZR 1M0D 1M0I 2PFJ 3CAE
ProteinModelPortal	P00641
SMR	P00641
DIP	DIP-41668N
IntAct	P00641
MINT	MINT-1513471
GeneID	1261079
KEGG	vg:1261079
EvolutionaryTrace	P00641
Proteomes	UP000000840
GO	GO:0008833 GO:0003677 GO:0015074 GO:0039657
Gene3D	3.40.91.30
InterPro	IPR008029 IPR011335 IPR011578
Pfam	PF05367
SUPFAM	SSF52980

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:1990238	double-stranded DNA endodeoxyribonuclease activity	PMID:5263754	ECO:0000315			F		Figure 1 shows significant degradation of both double and single stranded DNA upon infection with T7. The researchers, therefore, mutated a number of genes, and found out that only the mutants in genes 1 or 3 did not induce an increased endonuclease activity. It is, furthermore, stated that the product of gene 1 (RNA polymerase factor) is needed for the transcription of most of the T7 genome. Mutants in gene 3 were still synthesizing all essential T7 proteins, only lacking the increased endonuclease activity.	complete CACAO 11613
	GO:1990238	double-stranded DNA endodeoxyribonuclease activity	PMID:5541763	ECO:0000315			F		Figure 1 shows endonuclease activity after T7 infection of E. coli B. The endonuclease activity was not seen after infection with a mutant in gene 3 (Table 1).	complete CACAO 11615
	GO:1990238	double-stranded DNA endodeoxyribonuclease activity	PMID:5541763	ECO:0000314			F		The enzyme was purified and tested against various sources DNA. Breaks in the DNA strands were found (3-4 breaks per single strand before double strand breakage (see Figure 3).	complete CACAO 11617
	GO:0008821	crossover junction endodeoxyribonuclease activity	PMID:9236119	ECO:0000314			F		After binding to the DNA junction as a dimer, the two subunits of the T7 endonuclease 1 catalyze cleavages independently but rather simultaneously before either is released from the junction (within the lifetime of the protein-junction complex) (Figure 6 and text around it, and Discussion). Similar characteristics have been found in T4 endonuclease VII.	complete CACAO 11618
involved_in	GO:0090305	nucleic acid phosphodiester bond hydrolysis	GO_REF:0000108	ECO:0000366	evidence based on logical inference from automatic annotation used in automatic assertion	GO:0004519	P		Seeded From UniProt	complete
involved_in	GO:0090305	nucleic acid phosphodiester bond hydrolysis	GO_REF:0000108	ECO:0000366	evidence based on logical inference from automatic annotation used in automatic assertion	GO:0004518	P		Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR011578	F		Seeded From UniProt	complete
enables	GO:0004536	deoxyribonuclease activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR011578	F		Seeded From UniProt	complete
enables	GO:0008833	deoxyribonuclease IV (phage-T4-induced) activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR008029	F		Seeded From UniProt	complete
involved_in	GO:0015074	DNA integration	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR008029	P		Seeded From UniProt	complete
involved_in	GO:0016032	viral process	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR008029	P		Seeded From UniProt	complete
enables	GO:0008833	deoxyribonuclease IV (phage-T4-induced) activity	GO_REF:0000003	ECO:0000501	evidence used in automatic assertion	EC:3.1.21.2	F		Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0238	F		Seeded From UniProt	complete
enables	GO:0004518	nuclease activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0540	F		Seeded From UniProt	complete
involved_in	GO:0039657	suppression by virus of host gene expression	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1190	P		Seeded From UniProt	complete
enables	GO:0016787	hydrolase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0378	F		Seeded From UniProt	complete
enables	GO:0004519	endonuclease activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0255	F		Seeded From UniProt	complete
involved_in	GO:0016032	viral process	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0945	P		Seeded From UniProt	complete
involved_in	GO:0099015	degradation of host chromosome by virus	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1247	P		Seeded From UniProt	complete
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References

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