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PMID:15150235
| Citation |
Kenny, JG, McGrath, S, Fitzgerald, GF and van Sinderen, D (2004) Bacteriophage Tuc2009 encodes a tail-associated cell wall-degrading activity. J. Bacteriol. 186:3480-91 |
|---|---|
| Abstract |
Tuc2009 is a P335-type member of the tailed-phage supergroup Siphoviridae and was originally identified as a resident prophage of the gram-positive bacterium Lactococcus lactis UC509. A Tuc2009 gene designated tal2009 which is located within the morphogenic module was shown to specify a lytic activity within the 3' portion of its coding region. Comparative sequence analysis indicated that the cell wall-degrading part of Tal2009 is a member of the M37 protein family and that Tal2009 lacks a cell-binding domain, a finding supported by binding studies. Tal2009 appears to undergo self-mediated posttranslational processing in both L. lactis and Escherichia coli. Antibodies directed against a purified C-terminal portion of Tal2009 were used for immunoelectron microscopy, which showed that Tal2009 is located at the tail tip of Tuc2009. Antibody neutralization studies demonstrated that Tal2009-directed antibodies inhibited the ability of phage to mediate host lysis by more than 100-fold. These data indicate that tal2009 encodes a tail-associated lysin involved in localized cell wall degradation, thus allowing the Tuc2009 DNA injection machinery access to the membrane of its bacterial host. |
| Links |
PubMed PMC415775 Online version:10.1128/JB.186.11.3480-3491.2004 |
| Keywords |
Catalysis; Cell Wall/metabolism; Enzymes/chemistry; Lactococcus lactis/virology; Prophages/enzymology; Protein Processing, Post-Translational; Siphoviridae/enzymology; Viral Proteins/chemistry; Viral Proteins/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0098023: virus tail, tip |
ECO:0000314: |
C |
figure 6 shows gene product specific antibody binding to the tip of the phage tail |
complete | ||||
| GO:0008933: lytic transglycosylase activity |
ECO:0000314: |
F |
Assays were performed to visualize lytic functionality and as figure 5 shows, this gene seems to encode a tail-associated lysin, involved in cell wall degradation. |
complete | ||||
Notes
See also
References
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