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9CAUD:A0A0K2CZV8

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Species (Taxon ID) Bacillus phage TsarBomba. (1690456)
Gene Name(s) No Information Provided.
Protein Name(s) DNA primase (ECO:0000313 with EMBL:ALA13003.1)
External Links
UniProt A0A0K2CZV8
EMBL KT224359
RefSeq YP_009206931.1
GeneID 26633225
GO GO:0003677
GO:0003896
GO:0008270
Gene3D 3.90.580.10
InterPro IPR002694
Pfam PF01807
SMART SM00400

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0003896

DNA primase activity

PMID:11279245[1]

ECO:0000247

PMID:11279245[1] UniProtKB:P03692


F

"An altered gene 4 protein that does not form functional hexamers and consequently lacks detectable DNA unwinding activity was created. Remarkably, this monomeric primase readily primes DNA synthesis by T7 DNA polymerase on single-stranded templates. The monomeric gene 4 protein forms a specific and stable complex with T7 DNA polymerase and thereby delivers the RNA primer to the polymerase for the onset of DNA synthesis. These results show that a single subunit of the primase-helicase hexamer contains all of the residues required for primer synthesis and for utilization of primers by T7 DNA polymerase."

complete
CACAO 11467

GO:0003896

DNA primase activity

GO_REF:0000100

ECO:0000250

PMID:PMC3853052 PMID:PMC4299906 UniProtKB:A0A0A0RSK4 UniProtKB:U5PX97


F

BlastP shows e-values of 3e-140 or less depicting sequence similarity to Bacillus phage Moonbeam and Spock which have been identified to have DNA primase gene products. Moonbeam and Spock have homologous sequences for this gene therefore it can be assumed that the protein encoded by TsarBomba will have a similar enzymatic function

complete
CACAO 11469

GO:0008193

tRNA guanylyltransferase activity

PMID:23844012[2]

ECO:0000314

F

complete
CACAO 11470

involved_in

GO:0006269

DNA replication, synthesis of RNA primer

GO_REF:0000108

ECO:0000366

evidence based on logical inference from automatic annotation used in automatic assertion

GO:0003896

P

Seeded From UniProt

complete

enables

GO:0003677

DNA binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR002694
InterPro:IPR036977

F

Seeded From UniProt

complete

enables

GO:0003896

DNA primase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR002694

F

Seeded From UniProt

complete

involved_in

GO:0006260

DNA replication

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR002694
InterPro:IPR036977

P

Seeded From UniProt

complete

enables

GO:0008270

zinc ion binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR002694
InterPro:IPR036977

F

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 Kato, M et al. (2001) A complex of the bacteriophage T7 primase-helicase and DNA polymerase directs primer utilization. J. Biol. Chem. 276 21809-20 PubMed GONUTS page
  2. Hyde, SJ et al. (2013) Structural studies of a bacterial tRNA(HIS) guanylyltransferase (Thg1)-like protein, with nucleotide in the activation and nucleotidyl transfer sites. PLoS ONE 8 e67465 PubMed GONUTS page