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9CAUD:A0A0K2CZV8
Contents
| Species (Taxon ID) | Bacillus phage TsarBomba. (1690456) | |
| Gene Name(s) | No Information Provided. | |
| Protein Name(s) | DNA primase (ECO:0000313 with EMBL:ALA13003.1) | |
| External Links | ||
| UniProt | A0A0K2CZV8 | |
| EMBL | KT224359 | |
| RefSeq | YP_009206931.1 | |
| GeneID | 26633225 | |
| GO | GO:0003677 GO:0003896 GO:0008270 | |
| Gene3D | 3.90.580.10 | |
| InterPro | IPR002694 | |
| Pfam | PF01807 | |
| SMART | SM00400 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0003896 |
DNA primase activity |
ECO:0000247 |
PMID:11279245[1] UniProtKB:P03692
|
F |
"An altered gene 4 protein that does not form functional hexamers and consequently lacks detectable DNA unwinding activity was created. Remarkably, this monomeric primase readily primes DNA synthesis by T7 DNA polymerase on single-stranded templates. The monomeric gene 4 protein forms a specific and stable complex with T7 DNA polymerase and thereby delivers the RNA primer to the polymerase for the onset of DNA synthesis. These results show that a single subunit of the primase-helicase hexamer contains all of the residues required for primer synthesis and for utilization of primers by T7 DNA polymerase." |
complete | ||||
| GO:0003896 |
DNA primase activity |
ECO:0000250 |
PMID:PMC3853052 PMID:PMC4299906 UniProtKB:A0A0A0RSK4 UniProtKB:U5PX97
|
F |
BlastP shows e-values of 3e-140 or less depicting sequence similarity to Bacillus phage Moonbeam and Spock which have been identified to have DNA primase gene products. Moonbeam and Spock have homologous sequences for this gene therefore it can be assumed that the protein encoded by TsarBomba will have a similar enzymatic function |
complete | ||||
| GO:0008193 |
tRNA guanylyltransferase activity |
ECO:0000314 |
F |
complete | ||||||
|
involved_in |
GO:0006269 |
DNA replication, synthesis of RNA primer |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0003896 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003896 |
DNA primase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006260 |
DNA replication |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008270 |
zinc ion binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Kato, M et al. (2001) A complex of the bacteriophage T7 primase-helicase and DNA polymerase directs primer utilization. J. Biol. Chem. 276 21809-20 PubMed GONUTS page
- ↑ Hyde, SJ et al. (2013) Structural studies of a bacterial tRNA(HIS) guanylyltransferase (Thg1)-like protein, with nucleotide in the activation and nucleotidyl transfer sites. PLoS ONE 8 e67465 PubMed GONUTS page
