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Cacao

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GO:0003896DNA primase activityPMID:11279245ISA: Inferred from Sequence Alignment PMID:11279245 UniProtKB:P03692 F
This annotation made on page: 9CAUD:A0A0K2CZV8
By: Amariam1 (group Team You can't B. cereus) on 2016-04-11 12:49:11 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeMnguy1,
Team Blue B
2016-03-30 16:18:28 CDT

I believe this paper performs mutagenesis on Helicase domain only to test the activity of primase-helicase complex. But the activity of helicase was shown via direct assay ( monitoring separation of DNA_RNA strands) to measure the activity of primase-helicase complex. aka the evidence code should be inferred from direct assay

2
ChallengeLeakyJungle,
?
2016-03-30 09:36:34 CDT

Needs to have a transfer annotation for Tsarbomba

2
Private
Assessment
JimHu2016-05-02 12:19:38 CDTYou need to be an instructor to view these notes.Unacceptable
Unique/Original
Public
Assessment
DanielRenfro2016-04-11 12:49:12 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003896 DNA primase activity PMID:11279245 ISA: Inferred from Sequence Alignment PMID:11279245

UniProtKB:P03692

F "An altered gene 4 protein that does not form functional hexamers and consequently lacks detectable DNA unwinding activity was created. Remarkably, this monomeric primase readily primes DNA synthesis by T7 DNA polymerase on single-stranded templates. The monomeric gene 4 protein forms a specific and stable complex with T7 DNA polymerase and thereby delivers the RNA primer to the polymerase for the onset of DNA synthesis. These results show that a single subunit of the primase-helicase hexamer contains all of the residues required for primer synthesis and for utilization of primers by T7 DNA polymerase." complete
CACAO 11467
on 9CAUD:A0A0K2CZV8
Flagged