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BPT4:PORTL
Contents
| Species (Taxon ID) | Enterobacteria phage T4 (Bacteriophage T4). (10665) | |
| Gene Name(s) | 20 | |
| Protein Name(s) | Portal protein gp20
Gene product 20 gp20 | |
| External Links | ||
| UniProt | P13334 | |
| EMBL | X16055 AF158101 M19085 | |
| PIR | JU0161 | |
| RefSeq | NP_049782.1 | |
| PDB | 3JA7 | |
| PDBsum | 3JA7 | |
| PRIDE | P13334 | |
| GeneID | 1258582 | |
| KEGG | vg:1258582 | |
| Proteomes | UP000009087 | |
| GO | GO:0020002 GO:0016020 GO:0019028 GO:0019012 GO:0046718 GO:0019076 | |
| InterPro | IPR010823 | |
| Pfam | PF07230 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0019012 |
virion |
ECO:0000314 |
C |
Figure 3. |
complete | |||||
| GO:0019073 |
viral DNA genome packaging |
ECO:0000314 |
P |
"In any case, a scrambled 24 residue peptide with the same amino acid composition (Figure 1(c)) was without effect on in vitropackaging by the same assay, thereby providing evidence, at least with the unpolymerized peptide, that the packaging inhibition observed was specific and dependent upon the gp20 peptide sequence" |
complete | |||||
| GO:0046718 |
viral entry into host cell |
ECO:0000314 |
P |
This protein aids in head assembly, genome packaging, and genome injection. The most common function is of a portal protein that aids in infection by forming a channel, connecting the phage to the membrane of the host bacteria. See Figure 5 for complete outline and analysis of function. |
complete | |||||
| GO:0046798 |
viral portal complex |
ECO:0000314 |
C |
This is a ring of proteins where DNA enters and exits the phages capsid. The structure was determined by cryo-electron microscopy (cryo-EM) Figure 1. |
complete | |||||
| GO:0020002 |
host cell plasma membrane |
ECO:0000314 |
C |
Figure 3: To examine the localization of the plasma-encoded gp20, researchers created a gp20 and GFP fusion protein. The gp20-GFP fusion protein and gp40, encoded by Plasmid pT20gfp-40, was transformed into E. coli BL21(DE3) cells. When analyzed using fluoromicroscopy, the researchers found that the fluorescence signal was found mostly at the cell periphery where clusters of fluorescence were observed with an apparently regular spacing between them. From this, they concluded that gp20 is membrane bound, either by itself or in an aggregated form. |
complete | |||||
| GO:0020002 |
host cell plasma membrane |
ECO:0000314 |
C |
Fig 7,Protease mapping of His-gp20 and His-gp20s, shows Both His-gp20 and His-gp20s were not digested by the protease mapping, compared with OmpA. So both proteins are most likely peripherally bound to the cytoplasmic surface of the inner membrane. |
complete | |||||
|
part_of |
GO:0019012 |
virion |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0099000 |
viral genome ejection through host cell envelope, contractile tail mechanism |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000674883 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0019072 |
viral genome packaging |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000674883 |
P |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0019028 |
viral capsid |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000674883 |
C |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0019076 |
viral release from host cell |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000674883 |
P |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0020002 |
host cell plasma membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-1030 |
C |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0019028 |
viral capsid |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0033644 |
host cell membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0046718 |
viral entry into host cell |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016020 |
membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0099000 |
viral genome ejection through host cell envelope, contractile tail mechanism |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0019012 |
virion |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Clokie, MR et al. (2008) A proteomic approach to the identification of the major virion structural proteins of the marine cyanomyovirus S-PM2. Microbiology (Reading, Engl.) 154 1775-82 PubMed GONUTS page
- ↑ Lin, H et al. (1999) Analysis of capsid portal protein and terminase functional domains: interaction sites required for DNA packaging in bacteriophage T4. J. Mol. Biol. 289 249-60 PubMed GONUTS page
- ↑ Padilla-Sanchez, V et al. (2014) Structure-function analysis of the DNA translocating portal of the bacteriophage T4 packaging machine. J. Mol. Biol. 426 1019-38 PubMed GONUTS page
- ↑ Sun, L et al. (2015) Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution. Nat Commun 6 7548 PubMed GONUTS page
- ↑ 5.0 5.1 Quinten, TA & Kuhn, A (2012) Membrane interaction of the portal protein gp20 of bacteriophage T4. J. Virol. 86 11107-14 PubMed GONUTS page
