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User:Natjack1

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Phage Hunters Spring 2016

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptable9CAUD:A0A0K2CZZ12016-03-28 04:47:19 CDTGO:0004604 phosphoadenylyl-sulfate reductase (thioredoxin) activity (F)GO_REF:0000100ECO:0000250 sequence similarity evidence used in manual assertion

BlastP shows that the protein is a homolog of phosphoadenosine phosphosulfate reductase (WP_001466333.1 coded by E.coli). The conserved binding domains and sequence similarity provide support for the claim that this protein provides reductase enzymatic activity

challenge
unacceptable9CAUD:A0A0K2CZV82016-03-28 05:46:07 CDTGO:0003896 DNA primase activity (F)GO_REF:0000100ECO:0000250 sequence similarity evidence used in manual assertion

BlastP shows e-values of 3e-140 or less depicting sequence similarity to Bacillus phage Moonbeam and Spock which have been identified to have DNA primase gene products. Moonbeam and Spock have homologous sequences for this gene therefore it can be assumed that the protein encoded by TsarBomba will have a similar enzymatic function

challenge

acceptable:0
unacceptable:2
requires_changes:0
flagged:0

Annotations challenged by Natjack1

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorFrancis.Landman,
Team Spartan
ENTFC:VANSGO:0043433 - negative regulation of DNA-binding transcription factor activity (P)PMID:8981985ECO:0000316 genetic interaction evidence used in manual assertionchallengeC: 1
unacceptableKaitlynn.Jasken,
Team I'm bringing sexy bacterium
STRPY:O07480GO:0031167 - rRNA methylation (P)PMID:9527769ECO:0000247 sequence alignment evidence used in manual assertionchallengeC: 2

0 annotations fixed by Natjack1

What do the icons mean in the status column?

assessment icons.jpg