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PMID:2963141

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Citation

Arisaka, F, Ishimoto, L, Kassavetis, G, Kumazaki, T and Ishii, S (1988) Nucleotide sequence of the tail tube structural gene of bacteriophage T4. J. Virol. 62:882-6

Abstract

The nucleotide sequence of gene 19 of bacteriophage T4, the structural gene of the tail tube protein, was determined by both the dideoxy and the Maxam-Gilbert methods. The predicted Mr of tube protein gene product 19 is 18,842. The N-terminal amino acid of the tube protein was determined by Edman degradation, and the C-terminal sequence was confirmed by isolation of the C-terminal tryptic peptide. In the noncoding region between genes 18 and 19, there are two late-T4-promoter consensus sequences, 51 bases apart. The implication of the two late promoter sequences was examined by an S1 nuclease protection experiment. Both serve as weak promoters, but the bulk of the transcripts arise from further upstream of the two promoters.

Links

PubMed PMC253646

Keywords

Amino Acid Sequence; Base Sequence; Genes; Genes, Viral; Molecular Sequence Data; Nucleic Acid Conformation; T-Phages/genetics; Viral Proteins/genetics; Viral Tail Proteins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BPT4:TUBE

located_in

GO:0098026: virus tail, tube

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPT4:D9IEK2

GO:0019012: virion

ECO:0000314:

C

Figure 1

complete
CACAO 11677

BPT4:TUBE

part_of

GO:0098026: virus tail, tube

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

Notes

See also

References

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