Category:Team Purple A

Multiple genes encoding proteins similar to RecJ are found in eubacteria (Bacillus). Mutant deficient in RecJ nuclease show recombination deficiency.

Transfer annotation from page ECOLI:CRL. BlastP results show the e value of 3e -42 and query cover of 99% with E. Coli

Transfer annotation from page 9CAUD:G9J205. BlastP results shows e value of 5e -130, query cover of 100% with Bacillus Phage BCP78

As figure 2 shows, a series of plasmids were constructed each encoding a different alanine substitution. The complementation by sigma factor 70 C-terminal alanine substitution mutants was similar to the wild-type construct, indicating that the side-chains of most residues in C terminal region are not required for the general functions of sigma factor.

RNRs catalyze conversion of ribonucleotides to deoxyribonucleotides

From U5Q0Y2_9CAUD BlastP illustrations an e value of 1e-77, and query cover of 100% with bacillus spock

Transcriptional assays, as showed in Figure 5, were performed on super-coiled DNA template with and without CRP in the presence of RNA polymerase reconstituted either with wild type of different mutant of sigma factor 70. The analysis showed that CRP AR3+ activates RNA polymerase containing wild-type sigma factor 70 sixfold. However, RNA polymerase with mutant sigma factor were defective in activation by CRP AR3+.

Assays were performed to visualize lytic functionality and as figure 5 shows, this gene seems to encode a tail-associated lysin, involved in cell wall degradation.

BLASTP result indicates that the protein encoded by TSARBOMBA_192 is a homolog of ParM protein, the plasmid segregation actin-like motor protein in Bacteria (PMID:12065424, PMID:20844556), of Bacillus phage Troll orf220 (S5Y097) and Bacillus phage Riley orf215 (A0A075M4S3) with 95% Quer cover, 63% identity with e-value of 6e-166.

Several other closely related Bacillus phages were also found to contain genes that encode for plasmid segregation protein as shown on phylogeny tree based on the amino acid sequences in Figure 7A (PMID:25344242).

Examples of these include Plasmid segregation protein coded by Bacillus phage Spock orf209 (U5PXI1), Bacillus phage BigBertha orf214 (U5PVU6), and Putative plasmid segregation proteins coded by Bacillus phage Bp8p-T orf27 (A0A0A0PJ25), Bacillus phage Bp8p-C orf27 (A0A0A0PKY3).

This protein is the only one affected by the 3nt sequence differences (1 missense and 2 bp indel) between Bp8p-T (turbid) and Bp8p-C (clear) phages, which were separate isolates, but are essentially a mutant and wt version of the same phage.

The result from figure 7 indicates that truncation of putative plasmid segregation proteins coded by Bacillus phage Bp8p-T orf27 leads to decrease of the ATPase and polymerization abilities.

This protein is the only one affected by the 3nt sequence differences (1 missense and 2 bp indel) between Bp8p-T (turbid) and Bp8p-C (clear) phages, which were separate isolates, but are essentially a mutant and wt version of the same phage.

The result from Figure 7 indicates that truncation of putative plasmid segregation proteins coded by Bacillus phage Bp8p-C orf27 leads to changing in phage phenotype, decreasing in protein function, and higher rate of genome-packaging into phage capsid. These results imply direct relationship between the protein itself and lysogenic ability of the phage.

ParM is an actin-like protein which generates dynamic filaments that are necessary for Plasmid DNA segregation. The filament polymerization is activated only with the presence of ATP-bound form of ParM protein (PMID:20550930). Using the combined phase-contrast and immunofluorescence microscopy, figure 2C demonstrates the cells with mutation of ParM proteins fail to bind and hydrolyse ATP, leading to incorrect segregation of plasmid DNA.

Figure 2 Structure and Function of sigma2-3