PMID:25344242

Citation	Yuan, Y, Peng, Q, Wu, D, Kou, Z, Wu, Y, Liu, P and Gao, M (2015) Effects of actin-like proteins encoded by two Bacillus pumilus phages on unstable lysogeny, revealed by genomic analysis. Appl. Environ. Microbiol. 81:339-50
Abstract	We characterized two newly isolated myoviruses, Bp8p-C and Bp8p-T, infecting the ginger rhizome rot disease pathogen Bacillus pumilus GR8. The plaque of Bp8p-T exhibited a clear center with a turbid rim, suggesting that Bp8p-T could transform into latent phage. Lysogeny assays showed that both the two phages could form latent states, while Bp8p-T could form latent phage at a higher frequency and stability than Bp8p-C. The genomes of Bp8p-C and Bp8p-T were 151,417 and 151,419 bp, respectively; both encoded 212 putative proteins, and only differed by three nucleotides. Moreover, owing to this difference, Bp8p-C encoded a truncated, putative actin-like plasmid segregation protein Gp27-C. Functional analysis of protein Gp27 showed that Gp27-T encoded by Bp8p-T exhibited higher ATPase activity and assembly ability than Gp27-C. The results indicate that the difference in Gp27 affected the phage lysogenic ability. Structural proteome analysis of Bp8p-C virion resulted in the identification of 14 structural proteins, among which a pectin lyase-like protein, a putative poly-gamma-glutamate hydrolase, and three proteins with unknown function, were firstly identified as components of the phage virion. Both phages exhibited specific lytic ability to the host strain GR8. Bp8p-C showed better control effect on the pathogen in ginger rhizome slices than Bp8p-T, suggesting that Bp8p-C has a potential application in bio-control of ginger rhizome rot disease.
Links	PubMed PMC4272706 Online version:10.1128/AEM.02889-14
Keywords	Actins/genetics; Actins/metabolism; Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Bacillus/virology; Bacillus Phages/genetics; Bacillus Phages/isolation & purification; Bacillus Phages/physiology; Genome, Viral; Ginger/microbiology; Lysogeny; Molecular Sequence Data; Myoviridae/genetics; Myoviridae/isolation & purification; Myoviridae/physiology; Plant Diseases/microbiology; Sequence Analysis, DNA; Viral Plaque Assay; Viral Proteins/genetics; Viral Proteins/metabolism
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9CAUD:A0A0A0PKY3		GO:0030541: plasmid partitioning	ECO:0000315:		P		This protein is the only one affected by the 3nt sequence differences (1 missense and 2 bp indel) between Bp8p-T (turbid) and Bp8p-C (clear) phages, which were separate isolates, but are essentially a mutant and wt version of the same phage. The result from Figure 7 indicates that truncation of putative plasmid segregation proteins coded by Bacillus phage Bp8p-C orf27 leads to changing in phage phenotype, decreasing in protein function, and higher rate of genome-packaging into phage capsid. These results imply direct relationship between the protein itself and lysogenic ability of the phage.	complete CACAO 11560
9CAUD:A0A0A0PJ25		GO:0030541: plasmid partitioning	ECO:0000315:		P		This protein is the only one affected by the 3nt sequence differences (1 missense and 2 bp indel) between Bp8p-T (turbid) and Bp8p-C (clear) phages, which were separate isolates, but are essentially a mutant and wt version of the same phage. The result from figure 7 indicates that truncation of putative plasmid segregation proteins coded by Bacillus phage Bp8p-T orf27 leads to decrease of the ATPase and polymerization abilities.	complete CACAO 11561

References

See Help:References for how to manage references in GONUTS.