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ECOLI:CRL
Contents
| Species (Taxon ID) | Escherichia coli (strain K12). (83333) | |
| Gene Name(s) | crl | |
| Protein Name(s) | Sigma factor-binding protein Crl
Curlin genes transcriptional activatory protein | |
| External Links | ||
| UniProt | P24251 | |
| EMBL | V00316 X67207 U70214 U00096 AP009048 M13422 | |
| PIR | A64749 | |
| RefSeq | WP_000174689.1 | |
| ProteinModelPortal | P24251 | |
| SMR | P24251 | |
| BioGrid | 4260821 | |
| IntAct | P24251 | |
| STRING | 511145.b0240 | |
| EPD | P24251 | |
| PaxDb | P24251 | |
| PRIDE | P24251 | |
| EnsemblBacteria | BAA77909 | |
| KEGG | ecj:JW0230 | |
| PATRIC | 32115595 | |
| EchoBASE | EB1084 | |
| EcoGene | EG11092 | |
| eggNOG | ENOG4108UVK ENOG4111IQF | |
| HOGENOM | HOG000282284 | |
| KO | K11926 | |
| OMA | GWWLNLE | |
| OrthoDB | EOG64FKG6 | |
| BioCyc | EcoCyc:EG11092-MONOMER ECOL316407:JW0230-MONOMER | |
| PRO | PR:P24251 | |
| Proteomes | UP000000318 UP000000625 | |
| GO | GO:0005829 GO:0043623 GO:0045893 GO:0006351 | |
| HAMAP | MF_01178 | |
| InterPro | IPR009986 | |
| Pfam | PF07417 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0045893 |
positive regulation of transcription, DNA-templated |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0034622 |
cellular protein-containing complex assembly |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005829 |
cytosol |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0016987 |
sigma factor activity |
ECO:0000314 |
F |
Transcriptional assays, as showed in Figure 5, were performed on super-coiled DNA template with and without CRP in the presence of RNA polymerase reconstituted either with wild type of different mutant of sigma factor 70. The analysis showed that CRP AR3+ activates RNA polymerase containing wild-type sigma factor 70 sixfold. However, RNA polymerase with mutant sigma factor were defective in activation by CRP AR3+. |
complete | |||||
|
part_of |
GO:0005829 |
cytosol |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0016987 |
sigma factor activity |
ECO:0000314 |
F |
As figure 2 shows, a series of plasmids were constructed each encoding a different alanine substitution. The complementation by sigma factor 70 C-terminal alanine substitution mutants was similar to the wild-type construct, indicating that the side-chains of most residues in C terminal region are not required for the general functions of sigma factor. |
complete | |||||
|
involved_in |
GO:0045893 |
positive regulation of transcription, DNA-templated |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000100858 |
C |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0045893 |
positive regulation of transcription, DNA-templated |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000100858 |
P |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Typas, A et al. (2007) Stationary phase reorganisation of the Escherichia coli transcription machinery by Crl protein, a fine-tuner of sigmas activity and levels. EMBO J. 26 1569-78 PubMed GONUTS page
- ↑ Banta, AB et al. (2013) Key features of σS required for specific recognition by Crl, a transcription factor promoting assembly of RNA polymerase holoenzyme. Proc. Natl. Acad. Sci. U.S.A. 110 15955-60 PubMed GONUTS page
- ↑ Ishihama, Y et al. (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics 9 102 PubMed GONUTS page
- ↑ 4.0 4.1 Lonetto, MA et al. (1998) Identification of a contact site for different transcription activators in region 4 of the Escherichia coli RNA polymerase sigma70 subunit. J. Mol. Biol. 284 1353-65 PubMed GONUTS page
- ↑ Arnqvist, A et al. (1992) The Crl protein activates cryptic genes for curli formation and fibronectin binding in Escherichia coli HB101. Mol. Microbiol. 6 2443-52 PubMed GONUTS page
