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9CAUD:A0A0K2D0I8

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Species (Taxon ID) Bacillus phage TsarBomba. (1690456)
Gene Name(s) No Information Provided.
Protein Name(s) Exopolyphosphatase (ECO:0000313 with EMBL:ALA13182.1)
External Links
UniProt A0A0K2D0I8
EMBL KT224359
RefSeq YP_009206963.1
GeneID 26633412

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0004309

exopolyphosphatase activity

PMID:24275100[1]

ECO:0000315

F

there is an evidence that Exopolyphosphat of Pseudomonas aeruginosa is essential for flagellum dependent swimming and swarming motility. the author evaluated a WT,DppX and DppxC strain on LB medium plate and incubated at 37uC for 24h. the swimming motility of Dppx was 55-75%less than WT. the Dppx strain also decrease the swarming motility: 65-80% with respect to WT.

complete
CACAO 11462

GO:0004309

exopolyphosphatase activity

PMID:24569519[2]

ECO:0000315

F

to test the motility and biofilm formation of C.jejuni delta ppx mutants, the author assessed the motility on 0.4% soft agar at 42C for 24h. motility was quantified by measuring the swarming zone around the stabbed area. the result indicate that deleting the gene ppx in C.jejuni affect the amount of biofilm, therefore its affect the motility. also, ppx/gppa genes play a role in transmission related phenotypes such as motility and biofilm formation in C.jejuni.

complete
CACAO 11488

GO:0004309

exopolyphosphatase activity

PMID:24569519[2]

ECO:0000317

UniProtKB:904677


F

the phylogenetic analysis using MEGA-5 showed that C. jejuni PPX2 clustered with E.coli and mycobacterium tuberculosis species. (fig.S1) also HHpred show that gene TSARBOMBA_148 homologues to Mycobacterium tuberculosis with e-value= 8.8e-28 and probable 99.96.

complete
CACAO 11490

GO:0004309

exopolyphosphatase activity

PMID:10498723[3]

ECO:0000315

F

Multiple genes encoding proteins similar to RecJ are found in eubacteria (Bacillus). Mutant deficient in RecJ nuclease show recombination deficiency.

complete
CACAO 11598

Notes

References

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  1. Gallarato, LA et al. (2014) Exopolyphosphatase of Pseudomonas aeruginosa is essential for the production of virulence factors, and its expression is controlled by NtrC and PhoB acting at two interspaced promoters. Microbiology (Reading, Engl.) 160 406-17 PubMed GONUTS page
  2. 2.0 2.1 Malde, A et al. (2014) Functional characterization of exopolyphosphatase/guanosine pentaphosphate phosphohydrolase (PPX/GPPA) of Campylobacter jejuni. Virulence 5 521-33 PubMed GONUTS page
  3. Sutera, VA Jr et al. (1999) Mutational analysis of the RecJ exonuclease of Escherichia coli: identification of phosphoesterase motifs. J. Bacteriol. 181 6098-102 PubMed GONUTS page