User:Nitika1

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	THEAQ:SIGA	2016-03-27 08:47:53 CDT	GO:0016987 sigma factor activity (F)	PMID:11931761	ECO:0000314 direct assay evidence used in manual assertion	Figure 2 Structure and Function of sigma2-3	challenge
unacceptable	9CAUD:G9J205	2016-04-10 09:32:49 CDT	GO:0008933 lytic transglycosylase activity (F)	PMID:15150235	ECO:0000314 direct assay evidence used in manual assertion	Assays were performed to visualize lytic functionality and as figure 5 shows, this gene seems to encode a tail-associated lysin, involved in cell wall degradation.	challenge
unacceptable	9CAUD:A0A0K2CZX6	2016-04-11 09:03:12 CDT	GO:0008933 lytic transglycosylase activity (F)	GO_REF:0000100	ECO:0000250 sequence similarity evidence used in manual assertion	Transfer annotation from page 9CAUD:G9J205. BlastP results shows e value of 5e -130, query cover of 100% with Bacillus Phage BCP78	challenge
unacceptable	ECOLI:CRL	2016-04-10 10:07:17 CDT	GO:0016987 sigma factor activity (F)	PMID:9878355	ECO:0000314 direct assay evidence used in manual assertion	Transcriptional assays, as showed in Figure 5, were performed on super-coiled DNA template with and without CRP in the presence of RNA polymerase reconstituted either with wild type of different mutant of sigma factor 70. The analysis showed that CRP AR3+ activates RNA polymerase containing wild-type sigma factor 70 sixfold. However, RNA polymerase with mutant sigma factor were defective in activation by CRP AR3+.	challenge
unacceptable	9CAUD:A0A0K2D0M5	2016-04-11 09:05:03 CDT	GO:0016987 sigma factor activity (F)	GO_REF:0000100	ECO:0000250 sequence similarity evidence used in manual assertion	Transfer annotation from page ECOLI:CRL. BlastP results show the e value of 3e -42 and query cover of 99% with E. Coli	challenge
unacceptable	ECOLI:CRL	2016-04-11 04:25:40 CDT	GO:0016987 sigma factor activity (F)	PMID:9878355	ECO:0000314 direct assay evidence used in manual assertion	As figure 2 shows, a series of plasmids were constructed each encoding a different alanine substitution. The complementation by sigma factor 70 C-terminal alanine substitution mutants was similar to the wild-type construct, indicating that the side-chains of most residues in C terminal region are not required for the general functions of sigma factor.	challenge

acceptable:0
unacceptable:5
requires_changes:0
flagged:0

Annotations challenged by Nitika1

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	All5, Team You can't B. cereus	9CAUD:A0A0K2CZV8	GO:0008193 - tRNA guanylyltransferase activity (F)	PMID:23844012	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 6
unacceptable	Amariam1, Team You can't B. cereus	9CAUD:A0A0K2D089	GO:0046798 - viral portal complex (C)	PMID:12239300	ECO:0000316 genetic interaction evidence used in manual assertion	challenge	C: 5
unacceptable	Chensi1, Team Red A	9CAUD:G0XM98	GO:0098025 - virus tail, baseplate (C)	PMID:21481199	ECO:0000247 sequence alignment evidence used in manual assertion	challenge	C: 4
updatedbyinstructor	Schuhmzp, Team Go Dogs Go	BPT7:DPOL	GO:0003887 - DNA-directed DNA polymerase activity (F)	PMID:23012374	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 3

0 annotations fixed by Nitika1

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