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BPT7:DPOL
Contents
| Species (Taxon ID) | Enterobacteria phage T7 (Bacteriophage T7). (10760) | |
| Gene Name(s) | No Information Provided. | |
| Protein Name(s) | DNA-directed DNA polymerase
DNA polymerase gp5 Gene product 5 Gp5 T7 DNA polymerase | |
| External Links | ||
| UniProt | P00581 | |
| EMBL | V01146 | |
| PIR | A00716 | |
| RefSeq | NP_041982.1 | |
| PDB | 1SKR 1SKS 1SKW 1SL0 1SL1 1SL2 1T7P 1T8E 1TK0 1TK5 1TK8 1TKD 1X9M 1X9S 1X9W 1ZYQ 2AJQ | |
| PDBsum | 1SKR 1SKS 1SKW 1SL0 1SL1 1SL2 1T7P 1T8E 1TK0 1TK5 1TK8 1TKD 1X9M 1X9S 1X9W 1ZYQ 2AJQ | |
| ProteinModelPortal | P00581 | |
| SMR | P00581 | |
| DIP | DIP-41665N | |
| IntAct | P00581 | |
| MINT | MINT-1513442 | |
| GeneID | 1261044 | |
| EvolutionaryTrace | P00581 | |
| Proteomes | UP000000840 | |
| GO | GO:0003677 GO:0003887 GO:0004527 GO:0006261 GO:0016032 | |
| Gene3D | 3.30.420.10 | |
| InterPro | IPR019760 IPR001098 IPR002298 IPR012337 | |
| Pfam | PF00476 | |
| PRINTS | PR00868 | |
| SMART | SM00482 | |
| SUPFAM | SSF53098 | |
| PROSITE | PS00447 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0003887 |
DNA-directed DNA polymerase activity |
ECO:0000314 |
F |
Fig 3 |
complete | |||||
| GO:0004527 |
exonuclease activity |
2703498:2703498 |
ECO:0000315 |
F |
The experiment shows that mutation at His residue within the gene cause inactive exonuclease. |
complete | ||||
| GO:0090592 |
DNA synthesis involved in DNA replication |
ECO:0000315 |
P |
Figure 2 compared the activities of both gp5/trx and its mutated form Gp5-Fbpneu/trx in the presence of gp4 by looking at how much nucleotide (dTMP) had been incorporated in a sample of dsDNA . According to the paper and from the results, Gp5-Fbpneu/trx did not mediate the synthesis in the presence of gp4 over a large range of concentrations of gp5/trx and gp4. This means that a mutated gp5 would result in disrupted DNA synthesis in the presence of gp4 as compared to a normal version of gp5, showing its critical function for DNA replication. |
complete | |||||
| GO:0004529 |
exodeoxyribonuclease activity |
ECO:0000315 |
F |
Table 3 (specifically k_exo) compares the rates of exonuclease activity between the wild-type enzyme (105 1/s) and mutant enzyme (6.9e-4 1/s), revealing that the wild-type exonuclease rates are dramatically higher than that of the mutant enzyme's exonuclease rates. This shows that this domain of DNA polymerase has exonuclease activity. |
complete | |||||
|
enables |
GO:0008408 |
3'-5' exonuclease activity |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0071897 |
DNA biosynthetic process |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0003887 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0071897 |
DNA biosynthetic process |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0003887 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0071897 |
DNA biosynthetic process |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0003887 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0071897 |
DNA biosynthetic process |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0003887 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0090305 |
nucleic acid phosphodiester bond hydrolysis |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0008408 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0090305 |
nucleic acid phosphodiester bond hydrolysis |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0008408 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0090305 |
nucleic acid phosphodiester bond hydrolysis |
ECO:0000364 |
evidence based on logical inference from manual annotation used in automatic assertion |
GO:0008408 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0090305 |
nucleic acid phosphodiester bond hydrolysis |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0004527 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0090305 |
nucleic acid phosphodiester bond hydrolysis |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0004518 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003676 |
nucleic acid binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003887 |
DNA-directed DNA polymerase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006260 |
DNA replication |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006261 |
DNA-dependent DNA replication |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008408 |
3'-5' exonuclease activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003887 |
DNA-directed DNA polymerase activity |
ECO:0000501 |
evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000351416 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0008408 |
3'-5' exonuclease activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000351416 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0000166 |
nucleotide binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000351416 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003887 |
DNA-directed DNA polymerase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000351416 |
F |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0008152 |
metabolic process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0004527 |
exonuclease activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006260 |
DNA replication |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0016032 |
viral process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003824 |
catalytic activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0004518 |
nuclease activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0046872 |
metal ion binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016787 |
hydrolase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016740 |
transferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0039693 |
viral DNA genome replication |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003887 |
DNA-directed DNA polymerase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016779 |
nucleotidyltransferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Tran, NQ et al. (2012) Thioredoxin, the processivity factor, sequesters an exposed cysteine in the thumb domain of bacteriophage T7 DNA polymerase. J. Biol. Chem. 287 39732-41 PubMed GONUTS page
- ↑ Zhang, H et al. (2011) Helicase-DNA polymerase interaction is critical to initiate leading-strand DNA synthesis. Proc. Natl. Acad. Sci. U.S.A. 108 9372-7 PubMed GONUTS page
- ↑ Patel, SS et al. (1991) Pre-steady-state kinetic analysis of processive DNA replication including complete characterization of an exonuclease-deficient mutant. Biochemistry 30 511-25 PubMed GONUTS page
- ↑ Tabor, S & Richardson, CC (1989) Selective inactivation of the exonuclease activity of bacteriophage T7 DNA polymerase by in vitro mutagenesis. J. Biol. Chem. 264 6447-58 PubMed GONUTS page
d
- GO:0003887 ! DNA-directed DNA polymerase activity
- GO:0006261 ! DNA-templated DNA replication
- GO:0003677 ! DNA binding
- GO:0071897 ! DNA biosynthetic process
- GO:0004529 ! DNA exonuclease activity
- GO:0006260 ! DNA replication
- GO:0090592 ! DNA synthesis involved in DNA replication
- dsDNA viruses, no RNA stage
n
