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9CAUD:A0A0K2CZS7
Contents
| Species (Taxon ID) | Bacillus phage TsarBomba. (1690456) | |
| Gene Name(s) | No Information Provided. | |
| Protein Name(s) | RNA polymerase sigma factor (ECO:0000313 with EMBL:ALA13054.1) | |
| External Links | ||
| UniProt | A0A0K2CZS7 | |
| EMBL | KT224359 | |
| RefSeq | YP_009206960.1 | |
| GeneID | 26633265 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0016987 |
sigma factor activity |
ECO:0000315 |
F |
The authors created a plasmid construct with a promoterless Lacz and a random segment of E.coli Genomic DNA and transformed E.coli with it. RNA polymerase Sigma E factor fuctions under heat shock conditions. The colonies were placed under heat shock conditions. Those expressing Lacz were selected. These then went through a further selection process in order to determine the genes the sigma factor regualtes and the promoters it target. They also used mutants of RseA, which antagonized the sigma factor, to determine if RseA also regulates those genes effected by the sigma factor, as well as finding more promoters acted on by the sigma factor. |
complete | |||||
| GO:0016987 |
sigma factor activity |
ECO:0000250 |
PMID:3052291[2] InterPro:IPR000943 InterPro:IPR007627
|
F |
From U5Q0Y2_9CAUD BlastP illustrations an e value of 1e-77, and query cover of 100% with bacillus spock |
complete | ||||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Dartigalongue, C et al. (2001) Characterization of the Escherichia coli sigma E regulon. J. Biol. Chem. 276 20866-75 PubMed GONUTS page
- ↑ Helmann, JD & Chamberlin, MJ (1988) Structure and function of bacterial sigma factors. Annu. Rev. Biochem. 57 839-72 PubMed GONUTS page
