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PMID:2943077
| Citation |
Kato, H, Fujisawa, H and Minagawa, T (1986) Subunit arrangement of the tail fiber of bacteriophage T3. Virology 153:80-6 |
|---|---|
| Abstract |
A tail fiber of phage T3 is a trimer of the product of gene 17 (gp17). Treatment of T3 phage particles with chymotrypsin resulted in cleavage of only the tail fiber protein, at a site near the distal end of the fiber, causing a decrease of about 10% in the size of gp17 in the treated virion. The N-terminal amino acid sequences of intact and cleaved tail fiber proteins were identical and corresponded to that deduced from the nucleotide sequence of gene 17 except for the absence of the initiation Met residue. These results indicate that cleavage of the tail fiber occurred near the C terminus and suggest that gp17 polypeptides are oriented parallel to each other in the tail fiber. Association of tail fibers with the tail involves the N-terminal region of gp17. Under mild conditions of SDS-polyacrylamide gel electrophoresis, intact tail fibers dissociated from virions but cleaved ones did not. The nucleotide sequences indicate that T3 and T7 gp17 contain many sites that are potentially sensitive to chymotrypsin. In fact, free tail fibers, purified from T3-infected cells, were cleaved to many smaller fragments by chymotrypsin. These results suggest that the attachment of the tail fibers to the tail may induce a change(s) in the configuration and/or arrangement of gp17 to mask the sensitive sites from cleavage by chymotrypsin. |
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| Keywords |
Amino Acid Sequence; Chymotrypsin/pharmacology; Electrophoresis, Polyacrylamide Gel; Molecular Weight; Protein Conformation; T-Phages/analysis; T-Phages/physiology; Viral Proteins/analysis; Viral Tail Proteins |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0098024: virus tail, fiber |
ECO:0000317: |
|
C |
"may induce a change(s) in the configuration and/or arrangement of gp17 to mask the sensitive sites from cleavage by chymotrypsin" |
complete | |||
Notes
See also
References
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- ↑ Kato, H et al. (1986) Subunit arrangement of the tail fiber of bacteriophage T3. Virology 153 80-6 PubMed GONUTS page
