9CAUD:Q8H9R7

Species (Taxon ID)	Pseudomonas phage PaP3. (188350)
Gene Name(s)	orf3 (ECO:0000313 with EMBL:AAL85569.1)
Protein Name(s)	Terminase (ECO:0000313 with EMBL:AAL85569.1)
External Links
UniProt	Q8H9R7
EMBL	AY078382
RefSeq	NP_775255.1
GeneID	2700601
KEGG	vg:2700601
Proteomes	UP000001163
InterPro	IPR004921
Pfam	PF03237

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0016032	viral process	PMID:3488191	ECO:0000314			P		"This packaging process is initiated by recognition and endonucleolytic cleavage of viral concatemeric DNA. Concatemeric DNA, which consists of head-to-tail unit-length molecules, is generally produced via recombination [21] or rolling-circle replication [34, 37]. Next, the cleaved DNA end is linked to the portal vertex of the empty prohead through specific interactions between the terminase and the portal protein [19, 20, 38, 41]. Thus, a packaging motor is assembled, which drives directional translocation of DNA into the prohead, powered by the energy of ATP hydrolysis."	complete CACAO 11593
	GO:0005198	structural molecule activity	PMID:3488191	ECO:0000314			F		p03 - large subunit "The single p03 peak in the chromatogram suggests that the recombinant PaP3 large terminase subunit exists in solution as a monomer, which is also the case for many other phages [13, 16]."	complete CACAO 11594
	GO:0098772	molecular function regulator	PMID:3488191	ECO:0000314			F		"these results establish that the PaP3 large terminase subunit possesses a specific endonucleolytic activity on the PaP3 cos sequence, while the small subunit has a stimulatory effect on this activity. These two subunits may act cooperatively on the cos site of multimeric replicating PaP3 DNA and introduce staggered nicks to generate the 20-base ssDNA cohesive ends of the mature phage genome."	complete CACAO 11595
	GO:0098009	virus terminase, large subunit	PMID:23011306	ECO:0000314			C		The Orf3 Gene has a terminase function. Terminase typically has two subunits, the smaller is responsible for binding the viral DNA, while the larger subunit is responsible primarily for DNA cleavage and translocation. This particular gene makes the protein p03, which is the larger subunit in the terminase process (Figure 1). When unassembled from p01, p03 can typically perform the binding function by itself, however, when performing endonucleolytic activity, efficiency was tested, and was found to perform higher when both p01 and p03 were present (Figure 4).	complete CACAO 11781
	GO:0016887	ATPase activity	PMID:23011306	ECO:0000314			F		ATPase Activity has been found to be largely associated with the large subunit in terminase in this phage. This has been found through sequence analysis, as well as experimentally. The ATPase activity from p03 gel filtration columns were analyzed and it was found that there is a direct, positive correlation between the amount of product made and the presence of p03. The ATPase product greatly increases with the addition of p01 (the small subunit of terminase) as well. See Figure 6.	complete CACAO 11958
	GO:0043493	viral terminase complex	PMID:23011306	ECO:0000314			C		The terminase function in this phage requires both of the proteins p01 and p03. The p01 protein in this phage corresponds to the small subunit of terminase, while p03 corresponds to the large subunit of terminase. Although p03 can function without p01, p01 cannot function without p03. However, the endonucleolytic activity of p03 functions more effectively with the presence of p01. Thus exhibiting a cos-dependent endonucleolytic activity. See Figure 3	complete CACAO 11964
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References

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