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BPDPK:Q8SD18
Contents
| Species (Taxon ID) | Pseudomonas phage phiKZ. (169683) | |
| Gene Name(s) | No Information Provided. | |
| Protein Name(s) | PHIKZ144 (ECO:0000313 with EMBL:AAL83045.1) | |
| External Links | ||
| UniProt | Q8SD18 | |
| EMBL | AF399011 | |
| RefSeq | NP_803710.1 | |
| PDB | 3BKH 3BKV | |
| PDBsum | 3BKH 3BKV | |
| ProteinModelPortal | Q8SD18 | |
| SMR | Q8SD18 | |
| CAZy | GH23 | |
| PRIDE | Q8SD18 | |
| GeneID | 1258427 | |
| EvolutionaryTrace | Q8SD18 | |
| Proteomes | UP000002098 | |
| Gene3D | 1.10.101.10 | |
| InterPro | IPR023346 IPR002477 IPR008258 | |
| Pfam | PF01471 PF01464 | |
| SUPFAM | SSF47090 SSF53955 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0044659 |
cytolysis by virus of host cell |
ECO:0000314 |
P |
Decrease in bacterial concentration of E. coli and P. aeruginosa compared to the gram positive controls shows cell lysis by gp144 (Figure 1A). |
complete | |||||
| GO:0008933 |
lytic transglycosylase activity |
ECO:0000314 |
F |
Table 1 shows lytic transglycosylase activity due to the presence of (1,6)-anhydro N-acetylmuramic muropeptides. |
complete | |||||
| GO:0006508 |
proteolysis |
ECO:0000250 |
|
P |
From first two paragraphs under the "Identification of φKZ gp175 as the morphogenetic protease" says "we used local implementations of the Sequence Analysis and Modeling System and HHpred.The E-value of this match was only 3, however the HHpred P-value, which takes secondary structure into account, was 2.5E-05. This match was solidified using a custom T4 gp21 HHM powerful enough to detect an HHM based on the U35 peptidase family at E-values of 3.5E-05. Members of the U35 peptidase family contain diverged, but known, homologs to the T4 protease, such as the HK97 protease." |
complete | ||||
| GO:0006508 |
proteolysis |
ECO:0000314 |
P |
Figure 2 shows the location of the phage DNA where the prohead protease cleaves. |
complete | |||||
| GO:0006508 |
proteolysis |
ECO:0000314 |
P |
Figure 1. SDS-Page and Mass Spec shows the size and boundaries of the genes cleaved by the prohead protease. |
complete | |||||
|
enables |
GO:0008933 |
lytic transglycosylase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0006508 |
proteolysis |
ECO:0000250 |
|
P |
Blastp with T4 phage shows correlation to prohead protease of T4 |
complete | ||||
|
involved_in |
GO:0044659 |
cytolysis by virus of host cell |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 1.2 1.3 Paradis-Bleau, C et al. (2007) Peptidoglycan lytic activity of the Pseudomonas aeruginosa phage phiKZ gp144 lytic transglycosylase. FEMS Microbiol. Lett. 266 201-9 PubMed GONUTS page
- ↑ 2.0 2.1 2.2 Thomas, JA et al. (2012) Extensive proteolysis of head and inner body proteins by a morphogenetic protease in the giant Pseudomonas aeruginosa phage φKZ. Mol. Microbiol. 84 324-39 PubMed GONUTS page
- ↑ Mesyanzhinov, VV et al. (2002) The genome of bacteriophage phiKZ of Pseudomonas aeruginosa. J. Mol. Biol. 317 1-19 PubMed GONUTS page
