User:Ken7Smith

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	HUMAN:MKL1	2018-02-13 13:52:48 CST	GO:0044319 wound healing, spreading of cells (P)	PMID:29391067	ECO:0000314 direct assay evidence used in manual assertion	Fig 2(b) and (c) shows that MKL-1 promoted more cell migration compared to a standard in a wound healing assay in HeLa cells. Organism: Human	challenge
acceptable	HUMAN:YAP1	2018-02-14 20:31:13 CST	GO:0010629 negative regulation of gene expression (P)	PMID:29403552	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 5 In the YAP siRNA-transfected group the levels of Bax, caspase 3 and p53 were notably increased compared to BL control that did have significant levels of expression. Organism: Human Assay type: western blot analysis	challenge
acceptable	HUMAN:YAP1	2018-02-15 01:09:33 CST	GO:0050679 positive regulation of epithelial cell proliferation (P)	PMID:29403552	ECO:0000315 mutant phenotype evidence used in manual assertion	“As shown in Fig. 2, compared with the controls, the Eca-109 cell proliferation ability was notably decreased in cells transfected with YAP siRNA. These data indicated that YAP inhibition was able to suppress the proliferation in Eca-109 cells.” Organism: Human Assay type: MTT assay	challenge
acceptable	HUMAN:YAP1	2018-02-15 01:52:24 CST	GO:1904036 negative regulation of epithelial cell apoptotic process (P)	PMID:29403552	ECO:0000315 mutant phenotype evidence used in manual assertion	“As observed in Fig. 3, the apoptosis rate of Eca-109 cells was significantly increased in the YAP siRNA-transfected group. Thus, these results indicated that YAP inhibition induced cell apoptosis in Eca-109 cells.” Organism: Human Assay type: FCM	challenge
acceptable	MOUSE:S27A4	2018-02-15 21:36:43 CST	GO:0062003 negative regulation of all-trans-retinyl-ester hydrolase, 11-cis retinol forming activity (P)	PMID:23407971	ECO:0000314 direct assay evidence used in manual assertion	“As shown in Fig. 2C, synthesis of 11cROL in the cells decreased as FATP4 expression levels increased.” UNIPROT name: S27A4 Organism: Mouse	challenge
acceptable	HUMAN:CRP	2018-02-16 16:15:32 CST	GO:0032945 negative regulation of mononuclear cell proliferation (P)	PMID:29202702	ECO:0000314 direct assay evidence used in manual assertion	“CRP decreased the numbers of Mono Mac 6 cells at 24 and 48 h (Fig. 1d, e).” Organism: Human	challenge
acceptable	HUMAN:TCPR2	2018-02-27 14:19:42 CST	GO:0032527 protein exit from endoplasmic reticulum (P)	PMID:26431026	ECO:0000315 mutant phenotype evidence used in manual assertion	TECPR2 is required for protein exit from ER.(Fig 6E) Organism: Human	challenge
acceptable	HUMAN:YAP1	2018-02-27 14:41:42 CST	GO:0010628 positive regulation of gene expression (P)	PMID:29403552	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 5 In the YAP siRNA-transfected group the levels of Bcl-2 and p-Erk were evidently decreased compared to BL. Organism: Human Assay type: western blot analysis	challenge
acceptable	HUMAN:SOX9	2018-03-27 13:49:15 CDT	GO:0000122 negative regulation of transcription by RNA polymerase II (P)	PMID:29503843	ECO:0000314 direct assay evidence used in manual assertion	Fig 2A and 2B, Sox9 down-regulates BMP2-induced expression of Smad7. Organism: Human	challenge
updatedbyinstructor	MOUSE:SMAD7	2018-04-10 14:46:01 CDT	GO:1903043 positive regulation of chondrocyte hypertrophy (P)	PMID:29503843	ECO:0000314 direct assay evidence used in manual assertion	“overexpression of Smad7 led to a significant expansion of the hypertrophic chondrocyte zone,” compared to just BMP2 Figure 5AB Organism: Mouse	challenge
acceptable	MOUSE:SMAD7	2018-04-10 17:10:47 CDT	GO:1902731 negative regulation of chondrocyte proliferation (P)	PMID:29503843	ECO:0000314 direct assay evidence used in manual assertion	“overexpression of Smad7 led to decreasing the proliferating chondrocyte zone” compared to just BMP2 (Figure 5Bb) Organism: Mouse	challenge
acceptable	HUMAN:SOX9	2018-04-11 10:55:13 CDT	GO:0030279 negative regulation of ossification (P)	PMID:29503843	ECO:0000314 direct assay evidence used in manual assertion	Fig; 4 A-c shows that mineral is significantly decreased with BMP2 and Sox9 compared to the control of just BMP2. Organism: Human	challenge
acceptable	MOUSE:SMAD7	2018-04-11 10:55:13 CDT	GO:0030279 negative regulation of ossification (P)	PMID:29503843	ECO:0000314 direct assay evidence used in manual assertion	Fig;4 A-c shows that mineral is significantly decreased with BMP2 and SMAD7 compared to the control of just BMP2. Organism: Mouse	challenge
acceptable	HUMAN:SOX9	2018-04-17 13:40:00 CDT	GO:0061036 positive regulation of cartilage development (P)	PMID:29503843	ECO:0000314 direct assay evidence used in manual assertion	“Alcian blue staining analysis indicates that Sox9 significantly promoted BMP2-induced cartilage formation from MSCs (Figure 4C-c)” Organism: Human	challenge
acceptable	HUMAN:SOX9	2018-04-17 13:50:00 CDT	GO:1902732 positive regulation of chondrocyte proliferation (P)	PMID:29503843	ECO:0000314 direct assay evidence used in manual assertion	At the endpoint we analyzed the cultured explants histologically and found that BMP2 promoted a coordinated expansion of cartilage zones at the growth plat, while Sox9 overexpression enhanced BMP2-induced expansion of proliferating chondrocyte zone (Figure 5Bb)” Organism: Human	challenge

acceptable:14
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Ken7Smith

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	CameronCriswell, Team I broke mitosis	ARATH:OE64M	GO:0030150 - protein import into mitochondrial matrix (P)	PMID:29374544	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1
acceptable	CameronCriswell, Team I broke mitosis	MOUSE:KDM1A	GO:0034648 - obsolete histone demethylase activity (H3-dimethyl-K4 specific) (F)	PMID:29370269	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1
acceptable	CameronCriswell, Team I broke mitosis	MOUSE:KDM1A	GO:0034649 - obsolete histone demethylase activity (H3-monomethyl-K4 specific) (F)	PMID:29370269	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2
updatedbyinstructor	Reiglej, Team Cincy BISA	HUMAN:MLP3C	GO:0032527 - protein exit from endoplasmic reticulum (P)	PMID:26431026	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 3 fixed by CameronCriswell
correctedthroughchallenges	CameronCriswell, Team I broke mitosis	MOUSE:TMPS3	GO:0010628 - positive regulation of gene expression (P)	PMID:23255163	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2 fixed by Ken7Smith
correctedthroughchallenges	CameronCriswell, Team I broke mitosis	MOUSE:TMPS3	GO:0010629 - negative regulation of gene expression (P)	PMID:23255163	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1 fixed by Ken7Smith
acceptable	CameronCriswell, Team I broke mitosis	MOUSE:KDM1A	GO:0045665 - negative regulation of neuron differentiation (P)	PMID:29370269	ECO:0000316 genetic interaction evidence used in manual assertion	challenge	C: 1
updatedbyinstructor	Rashid, Team Cincy BISA	MOUSE:MFS12	GO:0048022 - negative regulation of melanin biosynthetic process (P)	PMID:29025994	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2 fixed by Ken7Smith

3 annotations fixed by Ken7Smith

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