PMID:29403552

Citation	Cui, M and Li, Z (2018) Downregulation of YAP inhibits proliferation and induces apoptosis in Eca-109 cells. Exp Ther Med 15:1048-1052
Abstract	A previous study reported that Yes-associated protein (YAP) gene was overexpressed in esophageal squamous cell carcinoma (ESCC); however, the exact role of YAP in ESCC remains largely unclear. The present study aimed to investigate the effects of YAP inhibition on ESCC. In order to investigate the exact role of YAP in ESCC cells, a stable YAP low-expression ESCC cell line was established using YAP-small interfering RNA. MTT assay was performed to examine the cell proliferation ability, while flow cytometry were used to detect the cell apoptosis and cell cycle distribution. In addition, reverse transcription-quantitative polymerase chain reaction and western blot analysis were applied for mRNA and protein level detection, respectively. The results suggested that YAP gene inhibition significantly repressed the ECA-109 cell proliferation and induced cell apoptosis, whereas this inhibition had no significant effects on cell cycle. Furthermore, the expression levels of cell apoptosis-associated proteins were determined in the current study, and the data demonstrated that the B-cell lymphoma 2 (Bcl-2)/Bcl-2-associated X protein ratio and phosphorylated extracellular signal-regulated kinase expression were significantly reduced, while the p53 and caspase 3 levels were notably increased in YAP gene-inhibited ECA-109 cells. In conclusion, the current study revealed that YAP gene inhibition suppresses the proliferation and induces apoptosis in ECA-109 cells, indicating that the YAP gene serves as an oncogene in ESCC.
Links	PubMed PMC5780845 Online version:10.3892/etm.2017.5492
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:YAP1		GO:0010629: negative regulation of gene expression	ECO:0000315:		P		Fig 5 In the YAP siRNA-transfected group the levels of Bax, caspase 3 and p53 were notably increased compared to BL control that did have significant levels of expression. Organism: Human Assay type: western blot analysis	complete CACAO 13068
HUMAN:YAP1		GO:0050679: positive regulation of epithelial cell proliferation	ECO:0000315:		P		“As shown in Fig. 2, compared with the controls, the Eca-109 cell proliferation ability was notably decreased in cells transfected with YAP siRNA. These data indicated that YAP inhibition was able to suppress the proliferation in Eca-109 cells.” Organism: Human Assay type: MTT assay	complete CACAO 13069
HUMAN:YAP1		GO:1904036: negative regulation of epithelial cell apoptotic process	ECO:0000315:		P		“As observed in Fig. 3, the apoptosis rate of Eca-109 cells was significantly increased in the YAP siRNA-transfected group. Thus, these results indicated that YAP inhibition induced cell apoptosis in Eca-109 cells.” Organism: Human Assay type: FCM	complete CACAO 13070
HUMAN:YAP1		GO:0010628: positive regulation of gene expression	ECO:0000315:		P		Fig 5 In the YAP siRNA-transfected group the levels of Bcl-2 and p-Erk were evidently decreased compared to BL. Organism: Human Assay type: western blot analysis	complete CACAO 13079

References

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