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Category:Team Gene ACKman

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableHUMAN:SOX9Ken7Smith, Team Gene ACKman2018-04-17 13:50:00 CDTGO:1902732 positive regulation of chondrocyte proliferation (P)PMID:29503843ECO:0000314 direct assay evidence used in manual assertion

At the endpoint we analyzed the cultured explants histologically and found that BMP2 promoted a coordinated expansion of cartilage zones at the growth plat, while Sox9 overexpression enhanced BMP2-induced expansion of proliferating chondrocyte zone (Figure 5Bb)”

Organism: Human

challenge
acceptableHUMAN:SOX9Ken7Smith, Team Gene ACKman2018-04-17 13:40:00 CDTGO:0061036 positive regulation of cartilage development (P)PMID:29503843ECO:0000314 direct assay evidence used in manual assertion

“Alcian blue staining analysis indicates that Sox9 significantly promoted BMP2-induced cartilage formation from MSCs (Figure 4C-c)”

Organism: Human

challenge
acceptableHUMAN:SOX9Ken7Smith, Team Gene ACKman2018-04-11 10:55:13 CDTGO:0030279 negative regulation of ossification (P)PMID:29503843ECO:0000314 direct assay evidence used in manual assertion

Fig; 4 A-c shows that mineral is significantly decreased with BMP2 and Sox9 compared to the control of just BMP2.

Organism: Human

challenge
acceptableMOUSE:SMAD7Ken7Smith, Team Gene ACKman2018-04-11 10:55:13 CDTGO:0030279 negative regulation of ossification (P)PMID:29503843ECO:0000314 direct assay evidence used in manual assertion

Fig;4 A-c shows that mineral is significantly decreased with BMP2 and SMAD7 compared to the control of just BMP2.

Organism: Mouse

challenge
acceptableMOUSE:SMAD7Ken7Smith, Team Gene ACKman2018-04-10 17:10:47 CDTGO:1902731 negative regulation of chondrocyte proliferation (P)PMID:29503843ECO:0000314 direct assay evidence used in manual assertion

“overexpression of Smad7 led to decreasing the proliferating chondrocyte zone” compared to just BMP2 (Figure 5Bb)

Organism: Mouse

challenge
updatedbyinstructorMOUSE:SMAD7Ken7Smith, Team Gene ACKman2018-04-10 14:46:01 CDTGO:1903043 positive regulation of chondrocyte hypertrophy (P)PMID:29503843ECO:0000314 direct assay evidence used in manual assertion

“overexpression of Smad7 led to a significant expansion of the hypertrophic chondrocyte zone,” compared to just BMP2 Figure 5AB

Organism: Mouse

challenge
acceptableHUMAN:SOX9Ken7Smith, Team Gene ACKman2018-03-27 13:49:15 CDTGO:0000122 negative regulation of transcription by RNA polymerase II (P)PMID:29503843ECO:0000314 direct assay evidence used in manual assertion

Fig 2A and 2B, Sox9 down-regulates BMP2-induced expression of Smad7.

Organism: Human

challenge
acceptableHUMAN:YAP1Ken7Smith, Team Gene ACKman2018-02-27 14:41:42 CSTGO:0010628 positive regulation of gene expression (P)PMID:29403552ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 5 In the YAP siRNA-transfected group the levels of Bcl-2 and p-Erk were evidently decreased compared to BL.

Organism: Human Assay type: western blot analysis

challenge
acceptableHUMAN:TCPR2Ken7Smith, Team Gene ACKman2018-02-27 14:19:42 CSTGO:0032527 protein exit from endoplasmic reticulum (P)PMID:26431026ECO:0000315 mutant phenotype evidence used in manual assertion

TECPR2 is required for protein exit from ER.(Fig 6E)

Organism: Human

challenge
acceptableHUMAN:CRPKen7Smith, Team Gene ACKman2018-02-16 16:15:32 CSTGO:0032945 negative regulation of mononuclear cell proliferation (P)PMID:29202702ECO:0000314 direct assay evidence used in manual assertion

“CRP decreased the numbers of Mono Mac 6 cells at 24 and 48 h (Fig. 1d, e).”

Organism: Human

challenge
acceptableMOUSE:S27A4Ken7Smith, Team Gene ACKman2018-02-15 21:36:43 CSTGO:0062003 negative regulation of all-trans-retinyl-ester hydrolase, 11-cis retinol forming activity (P)PMID:23407971ECO:0000314 direct assay evidence used in manual assertion

“As shown in Fig. 2C, synthesis of 11cROL in the cells decreased as FATP4 expression levels increased.”

UNIPROT name: S27A4

Organism: Mouse

challenge
acceptableHUMAN:YAP1Ken7Smith, Team Gene ACKman2018-02-15 01:52:24 CSTGO:1904036 negative regulation of epithelial cell apoptotic process (P)PMID:29403552ECO:0000315 mutant phenotype evidence used in manual assertion

“As observed in Fig. 3, the apoptosis rate of Eca-109 cells was significantly increased in the YAP siRNA-transfected group. Thus, these results indicated that YAP inhibition induced cell apoptosis in Eca-109 cells.”

Organism: Human

Assay type: FCM

challenge
acceptableHUMAN:YAP1Ken7Smith, Team Gene ACKman2018-02-15 01:09:33 CSTGO:0050679 positive regulation of epithelial cell proliferation (P)PMID:29403552ECO:0000315 mutant phenotype evidence used in manual assertion

“As shown in Fig. 2, compared with the controls, the Eca-109 cell proliferation ability was notably decreased in cells transfected with YAP siRNA. These data indicated that YAP inhibition was able to suppress the proliferation in Eca-109 cells.”

Organism: Human

Assay type: MTT assay

challenge
acceptableHUMAN:YAP1Ken7Smith, Team Gene ACKman2018-02-14 20:31:13 CSTGO:0010629 negative regulation of gene expression (P)PMID:29403552ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 5 In the YAP siRNA-transfected group the levels of Bax, caspase 3 and p53 were notably increased compared to BL control that did have significant levels of expression.

Organism: Human Assay type: western blot analysis

challenge
updatedbyinstructorWHEAT:A0A024DAP6Ahmed8342, Team Gene ACKman2018-02-13 14:07:06 CSTGO:0060261 positive regulation of transcription initiation from RNA polymerase II promoter (P)PMID:24323502ECO:0000314 direct assay evidence used in manual assertion

Figure 7b TaHsfC2a

Triticum aestivum

challenge
acceptableHUMAN:MKL1Ken7Smith, Team Gene ACKman2018-02-13 13:52:48 CSTGO:0044319 wound healing, spreading of cells (P)PMID:29391067ECO:0000314 direct assay evidence used in manual assertion

Fig 2(b) and (c) shows that MKL-1 promoted more cell migration compared to a standard in a wound healing assay in HeLa cells.

Organism: Human

challenge
updatedbyinstructorWHEAT:A0A024DAJ6Ahmed8342, Team Gene ACKman2018-02-06 14:45:39 CSTGO:0060261 positive regulation of transcription initiation from RNA polymerase II promoter (P)PMID:24323502ECO:0000314 direct assay evidence used in manual assertion

Common Wheat

Figure 7b TaHsfA4e

challenge
updatedbyinstructorWHEAT:A0A024DB95Ahmed8342, Team Gene ACKman2018-02-06 14:29:38 CSTGO:0060261 positive regulation of transcription initiation from RNA polymerase II promoter (P)PMID:24323502ECO:0000314 direct assay evidence used in manual assertion

Figure 7b TaHsfA2b

challenge

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Pages in category "Team Gene ACKman"

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