CACAO Spring 2014
My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
|---|
| acceptable | PSEAE:Q9I4H4 | 2014-04-05 11:50:03 CDT | GO:0006075 (1->3)-beta-D-glucan biosynthetic process (P) | PMID:23637868 | ECO:0000315 mutant phenotype evidence used in manual assertion | Figure 1A shows the different growth patterns shown in both the ndvB mutant and also in the wild type. Clearly, the wild type exhibits a higher growth capability due to the beta-glucan biosynthesis and sequestration of antibiotics in the periplasm.
| challenge |
| updatedbyinstructor | YEAST:BAP2 | 2014-04-06 16:43:26 CDT | GO:1990122 leucine import into cell (P) | PMID:24699373 | ECO:0000315 mutant phenotype evidence used in manual assertion | Figure 1A shows the OD concentrations of leucine from a cell for both wild types strains and for mutant strains. The wild type, with a BAP2 gene, has the highest OD of leucine and the mutants have decreased ODs.
| challenge |
| unacceptable | HUMAN:CXB2 | 2014-04-06 16:45:33 CDT | GO:0005243 gap junction channel activity (F) | PMID:24624091 | ECO:0000315 mutant phenotype evidence used in manual assertion | Figure 1 shows a much higher concentration of ions in components of the inner human ear for the mutant phenotype. However, in the wild type, with the functional Cx26, you see a much smaller ion concentration, indicating gap junction channel activity.
| challenge |
| unacceptable | CHLRE:A8IC80 | 2014-04-06 17:02:31 CDT | GO:0047714 galactolipase activity (F) | PMID:23161887 | ECO:0000315 mutant phenotype evidence used in manual assertion | Figure 1 shows the accumulation of triacylglycerides in both wild type and mutants. In the mutant knockout strain, we see a much lower percentage of triacylglycerols in the total fatty acid content. This indicates that the protein has galactolipase function that, in this case, is important following nitrogen deprivation.
| challenge |
| unacceptable | HUMAN:PHOP1 | 2014-04-06 17:23:32 CDT | GO:0044606 phosphocholine hydrolase activity (F) | PMID:24225136 | ECO:0000314 direct assay evidence used in manual assertion | Figure 1 shows some of the human phosphocholine hydrolase enzymes and the correlation between various cytotoxic t cell lines and apoptosis. Without the phosphocholine hydrolase activity of these enzymes, apoptosis wouldn't be induced and there would be no reduction in percent survival.
| challenge |
| requireschanges | HUMAN:VNN1 | 2014-04-06 17:27:17 CDT | GO:0017159 pantetheine hydrolase activity (F) | PMID:24140347 | ECO:0000315 mutant phenotype evidence used in manual assertion | In the article, Figure 2 shows that wild-type cells have much higher pantetheinase activity compared to mutant cells with Vnn1 knocked out, indicating that Vnn1 has pantetheine hydrolase activity.
| challenge |
acceptable:1
unacceptable:3
requires_changes:1
flagged:0
Annotations challenged by Bonifera
0 annotations fixed by Bonifera
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