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Category:MichSt14A 27

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesHUMAN:VNN1Bonifera, MichSt14A 272014-04-06 17:27:17 CDTGO:0017159 pantetheine hydrolase activity (F)PMID:24140347ECO:0000315 mutant phenotype evidence used in manual assertion

In the article, Figure 2 shows that wild-type cells have much higher pantetheinase activity compared to mutant cells with Vnn1 knocked out, indicating that Vnn1 has pantetheine hydrolase activity.

challenge
unacceptableHUMAN:PHOP1Bonifera, MichSt14A 272014-04-06 17:23:32 CDTGO:0044606 phosphocholine hydrolase activity (F)PMID:24225136ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows some of the human phosphocholine hydrolase enzymes and the correlation between various cytotoxic t cell lines and apoptosis. Without the phosphocholine hydrolase activity of these enzymes, apoptosis wouldn't be induced and there would be no reduction in percent survival.

challenge
unacceptableCHLRE:A8IC80Bonifera, MichSt14A 272014-04-06 17:02:31 CDTGO:0047714 galactolipase activity (F)PMID:23161887ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1 shows the accumulation of triacylglycerides in both wild type and mutants. In the mutant knockout strain, we see a much lower percentage of triacylglycerols in the total fatty acid content. This indicates that the protein has galactolipase function that, in this case, is important following nitrogen deprivation.

challenge
unacceptableHUMAN:CXB2Bonifera, MichSt14A 272014-04-06 16:45:33 CDTGO:0005243 gap junction channel activity (F)PMID:24624091ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1 shows a much higher concentration of ions in components of the inner human ear for the mutant phenotype. However, in the wild type, with the functional Cx26, you see a much smaller ion concentration, indicating gap junction channel activity.

challenge
updatedbyinstructorYEAST:BAP2Bonifera, MichSt14A 272014-04-06 16:43:26 CDTGO:1990122 leucine import into cell (P)PMID:24699373ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1A shows the OD concentrations of leucine from a cell for both wild types strains and for mutant strains. The wild type, with a BAP2 gene, has the highest OD of leucine and the mutants have decreased ODs.

challenge
acceptablePSEAE:Q9I4H4Bonifera, MichSt14A 272014-04-05 11:50:03 CDTGO:0006075 (1->3)-beta-D-glucan biosynthetic process (P)PMID:23637868ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1A shows the different growth patterns shown in both the ndvB mutant and also in the wild type. Clearly, the wild type exhibits a higher growth capability due to the beta-glucan biosynthesis and sequestration of antibiotics in the periplasm.

challenge

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Pages in category "MichSt14A 27"

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