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Category:Team Gene Queens

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SUPER BOWL ROUND Spring 2012

StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesECOLI:RSMARossira1, Team Gene Queens2012-04-15 14:22:09 CDTGO:0019104 DNA N-glycosylase activity (F)PMID:2673420ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows cleavage of nucleotides on miss-paired bases when KsgA is added.

challenge
requireschangesECOLI:MSBARossira1, Team Gene Queens2012-04-15 13:26:51 CDTGO:0004012 phospholipid-translocating ATPase activity (F)PMID:2888566ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows E.coli and different membranes, with and with out ATP and the distribution of phospholipid membranes when MsbA is incorporated into the membrane.

challenge
acceptableYEAS8:C8ZHG8Beebeje1, Team Gene Queens2012-04-14 22:54:55 CDTGO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA (P)PMID:9482745ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows that Dcp1p is sufficient to remove the m7Gpp cap from mRNA.

challenge
acceptableAGRRK:B9J8G0Beebeje1, Team Gene Queens2012-04-14 22:41:09 CDTGO:0000773 phosphatidyl-N-methylethanolamine N-methyltransferase activity (F)PMID:19181804ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows that in the absence of pmtA PE is not converted to PC by transmethylation reactions. While in the presence of pmtA it is converted to intermediates of the pathway and PC.

challenge
acceptableSCHPO:RAD3Rossira1, Team Gene Queens2012-04-11 14:09:28 CDTGO:0004672 protein kinase activity (F)PMID:22302936ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2A,E show that mutants without Rad3 were unable to phosphorylate Thr93 of Ccq1 and those with Rad3 were able to phosphorylate Thr93 of Ccq1.

challenge
acceptableSCHPO:ATMRossira1, Team Gene Queens2012-04-11 14:05:42 CDTGO:0004672 protein kinase activity (F)PMID:22302936ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 show that Tel 1 is required for phosphorylation of Thr 93 of Ccq1. Mutants without Tel 1 were unable to phosphorylate Ccq1.

challenge
requireschangesSCHPO:CCQ1Rossira1, Team Gene Queens2012-04-11 13:52:38 CDTGO:0071573 telosome assembly (P)PMID:19141478ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1 shows that mutants without Ccq1 are unable to recruit telomerase to the telomerase in vivo.

challenge
requireschangesCLOSR:Q8GJ42Beebeje1, Team Gene Queens2012-04-11 11:36:34 CDTGO:0045493 xylan catabolic process (P)PMID:15256568ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows the hydrolysis of xylobiose to xylose. Xylobiose is a Xylan degradation product that is further degraded to xylose.

challenge
requireschangesHUMAN:BACE1Rossira1, Team Gene Queens2012-04-11 09:52:09 CDTGO:0008233 peptidase activity (F)PMID:22449099ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1 shows that the activity of BACE-1 is actively involved in the cleave of ST6Gal I from the Golgi membrane inducing secretion. Mutants without BACE-1 activity could not induce the cleavage of ST6Gal I and no secretion occurred.

challenge
requireschangesSTAAW:CLPPRossira1, Team Gene Queens2012-04-10 11:51:05 CDTGO:0008462 endopeptidase Clp activity (F)PMID:21969594ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 shows septum formation inhibition in those wild type species treated with antibiotic, in mutant clp species septum formation is able to occur even when treated with an antibiotic.

challenge
acceptableSTAA8:ARCRBeebeje1, Team Gene Queens2012-04-10 11:48:24 CDTGO:0006355 regulation of transcription, DNA-dependent (P)PMID:17557828ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 shows that arcR is responsible for the transcription of genes within the arc operon under anaerobic conditions.

challenge
requireschangesSTAA8:ARCRBeebeje1, Team Gene Queens2012-04-10 11:43:33 CDTGO:1900079 regulation of arginine biosynthetic process (P)PMID:17557828ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 shows that arcR regulates the arginine biosynthetic process by controlling transcription of the arc operon.

challenge
acceptableHV1A2:NEFBeebeje1, Team Gene Queens2012-04-10 11:06:26 CDTGO:0007171 activation of transmembrane receptor protein tyrosine kinase activity (P)PMID:9218412ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows that when nef is present with hck it can induce tyrosine phosphorylation of endogenous proteins in wild type cells

challenge


MSU Internal Competition

StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschanges?Rossira1, Team Gene Queens2012-03-29 11:14:03 CDTGO:0008375 acetylglucosaminyltransferase activity (Fig 4. Shows and assay of recombinant species what oxt gene and product produced when substrate added)PMID:11929872IDA: Inferred from Direct Assay
challenge
requireschangesSTAAU:ARSCBeebeje1, Team Gene Queens2012-03-28 11:40:59 CDTGO:0055114 oxidation-reduction process (P)PMID:1409657ECO:0000316 genetic interaction evidence used in manual assertion

Figure 2: In this figure there is a strain that contains arsB, arsR, and arsC. This stain is capable of reducing arsenate to arsenite. The second strain used has mutations in both arsB and arsR but is still able to reduce arsenate indicating arsC is responsible for arsenate reduction.

challenge
updatedbyinstructorMOUSE:BCCIPRossira1, Team Gene Queens2012-03-27 12:07:45 CDTGO:0006974 cellular response to DNA damage stimulus (P)PMID:21966279ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 7. BCCIP Knockdown cells exhibit greater growth inhibition by irradiation compared to control.

challenge

Pages in category "Team Gene Queens"

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