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SCHPO:RAD3

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Species (Taxon ID) Schizosaccharomyces pombe (strain ATCC 38366 / 972) (Fission yeast). (taxon:284812)
Gene Name(s) rad3
Protein Name(s)
  • Protein kinase rad3
  • DNA repair protein rad3
External Links
UniProt Identifier RAD3_SCHPO
UniProt Accessions Q02099, Q92391, Q9UUM1,
EMBL Y09076, U76307, CU329671, X63544,
PIR T39911,
RefSeq NP_595357.1,
IntAct Q02099,
Pfam PF02259, PF02260, PF00454, PF08064,

Annotations

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005829

cytosol

IDA: Inferred from Direct Assay

C

Source: GeneDB_Spombe

GO:0000228

nuclear chromosome

IDA: Inferred from Direct Assay

C

Source: GeneDB_Spombe

GO:0043234

protein complex

NAS: Non-traceable Author Statement

C

Source: GeneDB_Spombe

GO:0005524

ATP binding

IEA: Inferred from Electronic Annotation

F

Source: UniProtKB-KW

GO:0005515

protein binding

IPI: Inferred from Physical Interaction

F

Source: IntAct

GO:0004674

protein serine/threonine kinase activity

IDA: Inferred from Direct Assay

F

Source: GeneDB_Spombe

GO:0006281

DNA repair

IEA: Inferred from Electronic Annotation

P

Source: UniProtKB-KW

GO:0006260

DNA replication

IEA: Inferred from Electronic Annotation

P

Source: UniProtKB-KW

GO:0031573

intra-S DNA damage checkpoint

IDA: Inferred from Direct Assay

P

Source: GeneDB_Spombe

GO:0007131

reciprocal meiotic recombination

IMP: Inferred from Mutant Phenotype

P

Source: GeneDB_Spombe

GO:0000166

nucleotide binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

GO:0004672

protein kinase activity

PMID:22302936[1]

IMP: Inferred from Mutant Phenotype

F

Figure 2A,E show that mutants without Rad3 were unable to phosphorylate Thr93 of Ccq1 and those with Rad3 were able to phosphorylate Thr93 of Ccq1.

complete

GO:0000228

nuclear chromosome

PMID:8843195[2]

IDA: Inferred from Direct Assay

C

GO:0004674

protein serine/threonine kinase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012993

F

GO:0004674

protein serine/threonine kinase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0723

F

GO:0004674

protein serine/threonine kinase activity

PMID:11553781[3]

IDA: Inferred from Direct Assay

F

GO:0005488

binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011990

F

GO:0005488

binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016024

F

GO:0005515

protein binding

PMID:10512862[4]

IPI: Inferred from Physical Interaction

GeneDB_Spombe:SPCC1259.13

F

GO:0005515

protein binding

PMID:14739927[5]

IPI: Inferred from Physical Interaction

UniProtKB:P34208

F

GO:0005515

protein binding

PMID:14739927[5]

IPI: Inferred from Physical Interaction

UniProtKB:P87074

F

GO:0005515

protein binding

PMID:15173168[6]

IPI: Inferred from Physical Interaction

GeneDB_Spombe:SPAC694.06c

F

GO:0005524

ATP binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

GO:0005634

nucleus

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0539

C

GO:0005634

nucleus

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0191

C

GO:0005829

cytosol

PMID:16823372[7]

IDA: Inferred from Direct Assay

C

GO:0006260

DNA replication

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0237

P

GO:0006281

DNA repair

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0234

P

GO:0006974

response to DNA damage stimulus

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0227

P

GO:0007131

reciprocal meiotic recombination

PMID:12628934[8]

IMP: Inferred from Mutant Phenotype

P

GO:0007346

regulation of mitotic cell cycle

GO_REF:0000001

NAS: Non-traceable Author Statement

P

GO:0016301

kinase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0418

F

GO:0016310

phosphorylation

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0418

P

GO:0016740

transferase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

GO:0016772

transferase activity, transferring phosphorus-containing groups

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011009

F

GO:0016773

phosphotransferase activity, alcohol group as acceptor

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000403

F

GO:0016773

phosphotransferase activity, alcohol group as acceptor

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018936

F

GO:0031573

intra-S DNA damage checkpoint

PMID:12032307[9]

IMP: Inferred from Mutant Phenotype

P

GO:0031573

intra-S DNA damage checkpoint

PMID:19037101[10]

IDA: Inferred from Direct Assay

P

GO:0043234

protein complex

GO_REF:0000001

NAS: Non-traceable Author Statement

C


Notes

References

See Help:References for how to manage references in GONUTS.

  1. Yamazaki, H et al. (2012) Tel1(ATM) and Rad3(ATR) phosphorylate the telomere protein Ccq1 to recruit telomerase and elongate telomeres in fission yeast. Genes Dev. 26 241-6 PubMed GONUTS page
  2. Keegan, KS et al. (1996) The Atr and Atm protein kinases associate with different sites along meiotically pairing chromosomes. Genes Dev. 10 2423-37 PubMed GONUTS page
  3. Lopez-Girona, A et al. (2001) Serine-345 is required for Rad3-dependent phosphorylation and function of checkpoint kinase Chk1 in fission yeast. Proc. Natl. Acad. Sci. U.S.A. 98 11289-94 PubMed GONUTS page
  4. Chapman, CR et al. (1999) Requirement of sequences outside the conserved kinase domain of fission yeast Rad3p for checkpoint control. Mol. Biol. Cell 10 3223-38 PubMed GONUTS page
  5. 5.0 5.1 Mochida, S et al. (2004) Regulation of checkpoint kinases through dynamic interaction with Crb2. EMBO J. 23 418-28 PubMed GONUTS page
  6. Tanaka, K & Russell, P (2004) Cds1 phosphorylation by Rad3-Rad26 kinase is mediated by forkhead-associated domain interaction with Mrc1. J. Biol. Chem. 279 32079-86 PubMed GONUTS page
  7. Matsuyama, A et al. (2006) ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol. 24 841-7 PubMed GONUTS page
  8. Prudden, J et al. (2003) Pathway utilization in response to a site-specific DNA double-strand break in fission yeast. EMBO J. 22 1419-30 PubMed GONUTS page
  9. Marchetti, MA et al. (2002) A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway. Proc. Natl. Acad. Sci. U.S.A. 99 7472-7 PubMed GONUTS page
  10. Willis, N & Rhind, N (2009) Mus81, Rhp51(Rad51), and Rqh1 form an epistatic pathway required for the S-phase DNA damage checkpoint. Mol. Biol. Cell 20 819-33 PubMed GONUTS page