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PMID:22302936
| Citation |
Yamazaki, H, Tarumoto, Y and Ishikawa, F (2012) Tel1(ATM) and Rad3(ATR) phosphorylate the telomere protein Ccq1 to recruit telomerase and elongate telomeres in fission yeast. Genes Dev. 26:241-6 |
|---|---|
| Abstract |
In fission yeast, the DNA damage sensor kinases Tel1(ATM) and Rad3(ATR) exist at telomeres and are required for telomere maintenance, but the biological role they play at telomeres is not known. Here we show that the telomere protein Ccq1 is phosphorylated at Thr 93 (threonine residue at amino acid 93) by Tel1(ATM) and Rad3(ATR) both in vitro and in vivo. A ccq1 mutant in which alanine was substituted for Thr 93 failed to recruit telomerase to telomeres and showed gradual shortening of telomeres. These results indicate that the direct phosphorylation of Ccq1 Thr 93 by Tel1 and Rad3 is involved in the recruitment of telomerase to elongate telomeres. |
| Links |
PubMed PMC3278891 Online version:10.1101/gad.177873.111 |
| Keywords |
Cell Cycle Proteins/metabolism; Mutation; Phosphorylation; Protein Kinases/metabolism; Protein-Serine-Threonine Kinases/metabolism; Schizosaccharomyces/enzymology; Schizosaccharomyces/genetics; Schizosaccharomyces pombe Proteins/genetics; Schizosaccharomyces pombe Proteins/metabolism; Telomerase/metabolism; Telomere/enzymology; Telomere/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0004672: protein kinase activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0004672: protein kinase activity |
ECO:0000315: |
F |
Figure 2 show that Tel 1 is required for phosphorylation of Thr 93 of Ccq1. Mutants without Tel 1 were unable to phosphorylate Ccq1. |
complete | ||||
|
enables |
GO:0004672: protein kinase activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0004672: protein kinase activity |
ECO:0000315: |
F |
Figure 2A,E show that mutants without Rad3 were unable to phosphorylate Thr93 of Ccq1 and those with Rad3 were able to phosphorylate Thr93 of Ccq1. |
complete | ||||
See also
References
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