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ECOLI:MSBA

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Species (Taxon ID) Escherichia coli (strain K12). (83333)
Gene Name(s) msbA (ECO:0000255 with HAMAP-Rule:MF_01703)
Protein Name(s) Lipid A export ATP-binding/permease protein MsbA (ECO:0000255 with HAMAP-Rule:MF_01703)
External Links
UniProt P60752
EMBL Z11796
U00096
AP009048
PIR C85617
E90753
S27998
RefSeq NP_415434.1
YP_489186.1
PDB 3B5W
PDBsum 3B5W
DisProt DP00400
ProteinModelPortal P60752
SMR P60752
DIP DIP-36229N
IntAct P60752
MINT MINT-1277336
STRING 511145.b0914
TCDB 3.A.1.106.1
PaxDb P60752
PRIDE P60752
EnsemblBacteria AAC74000
BAA35658
GeneID 12931019
945530
KEGG ecj:Y75_p0886
eco:b0914
PATRIC 32117041
EchoBASE EB0608
EcoGene EG10613
eggNOG COG1132
InParanoid P60752
KO K11085
OMA ENCAVVS
OrthoDB EOG6T7N3V
PhylomeDB P60752
BioCyc EcoCyc:EG10613-MONOMER
ECOL316407:JW0897-MONOMER
MetaCyc:EG10613-MONOMER
EvolutionaryTrace P60752
PRO PR:P60752
Proteomes UP000000318
UP000000625
Genevestigator P60752
GO GO:0043190
GO:0016020
GO:0005524
GO:0008144
GO:0008289
GO:0005319
GO:0034040
GO:0015437
GO:1901264
GO:0006869
Gene3D 3.40.50.300
InterPro IPR003593
IPR011527
IPR003439
IPR017871
IPR011917
IPR027417
Pfam PF00664
PF00005
SMART SM00382
SUPFAM SSF52540
SSF90123
TIGRFAMs TIGR02203
PROSITE PS50929
PS00211
PS50893
PS51239

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0004012

phospholipid-translocating ATPase activity

PMID:PMC2888566

ECO:0000314

F

Figure 2 shows E.coli and different membranes, with and with out ATP and the distribution of phospholipid membranes when MsbA is incorporated into the membrane.

complete
CACAO 4879

part_of

GO:0016020

membrane

PMID:16858726[1]

ECO:0007005

high throughput direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

enables

GO:0042626

ATPase activity, coupled to transmembrane movement of substances

PMID:21873635[2]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG10613
EcoGene:EG11405
FB:FBgn0004513
MGI:MGI:97570
MGI:MGI:98483
PANTHER:PTN002517083
PomBase:SPBC25B2.02c
PomBase:SPCC737.09c
SGD:S000004916
TAIR:locus:2041434
UniProtKB:A0A1D8PKI5
UniProtKB:P21439
UniProtKB:Q03519
UniProtKB:Q9NP58
UniProtKB:Q9NP78
ZFIN:ZDB-GENE-080204-52

F

Seeded From UniProt

complete

part_of

GO:0016021

integral component of membrane

PMID:21873635[2]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG10613
EcoGene:EG11405
FB:FBgn0004513
MGI:MGI:1924956
PANTHER:PTN002517083
PomBase:SPCC737.09c
RGD:3817
RGD:3818
UniProtKB:Q03518
UniProtKB:Q03519
UniProtKB:Q2M3G0
UniProtKB:Q8IDT6
UniProtKB:Q9NP58
UniProtKB:Q9NP78
UniProtKB:Q9NRK6
WB:WBGene00001815

C

Seeded From UniProt

complete

enables

GO:0015238

drug transmembrane transporter activity

PMID:21873635[2]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

EcoGene:EG10613
PANTHER:PTN001954199
UniProtKB:Q9HUG8

F

Seeded From UniProt

complete

enables

GO:0042802

identical protein binding

PMID:25661651[3]

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:P60752

F

Seeded From UniProt

complete

enables

GO:0042802

identical protein binding

PMID:18024585[4]

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:P60752

F

Seeded From UniProt

complete

enables

GO:0042802

identical protein binding

PMID:16858726[1]

ECO:0000353

physical interaction evidence used in manual assertion

UniProtKB:P60752

F

Seeded From UniProt

complete

enables

GO:0034040

lipid-transporting ATPase activity

PMID:18344567[5]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0008289

lipid binding

PMID:18344567[5]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0008144

drug binding

PMID:18344567[5]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

part_of

GO:0043190

ATP-binding cassette (ABC) transporter complex

PMID:18024585[4]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0043190

ATP-binding cassette (ABC) transporter complex

PMID:8809774[6]

ECO:0000245

automatically integrated combinatorial evidence used in manual assertion

C

Seeded From UniProt

complete

involved_in

GO:0034204

lipid translocation

PMID:20412049[7]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0034204

lipid translocation

PMID:15304478[8]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0015920

lipopolysaccharide transport

PMID:11278265[9]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

enables

GO:0015437

lipopolysaccharide-transporting ATPase activity

PMID:20412049[7]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0015437

lipopolysaccharide-transporting ATPase activity

PMID:12119303[10]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0008559

xenobiotic transmembrane transporting ATPase activity

PMID:12842882[11]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

part_of

GO:0005887

integral component of plasma membrane

PMID:8809774[6]

ECO:0000255

match to sequence model evidence used in manual assertion

C

Seeded From UniProt

Missing: with/from

part_of

GO:0005886

plasma membrane

PMID:8809774[6]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005886

plasma membrane

PMID:17309111[12]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005886

plasma membrane

PMID:15919996[13]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005886

plasma membrane

PMID:15911532[14]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

enables

GO:0005524

ATP binding

PMID:8809774[6]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0005524

ATP binding

PMID:8809774[6]

ECO:0000255

match to sequence model evidence used in manual assertion

F

Seeded From UniProt

Missing: with/from

involved_in

GO:0006855

drug transmembrane transport

GO_REF:0000108

ECO:0000364

evidence based on logical inference from manual annotation used in automatic assertion

GO:0008559

P

Seeded From UniProt

complete

involved_in

GO:0006855

drug transmembrane transport

GO_REF:0000108

ECO:0000364

evidence based on logical inference from manual annotation used in automatic assertion

GO:0015238

P

Seeded From UniProt

complete

involved_in

GO:0042908

xenobiotic transport

GO_REF:0000108

ECO:0000364

evidence based on logical inference from manual annotation used in automatic assertion

GO:0008559

P

Seeded From UniProt

complete

enables

GO:0005524

ATP binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR003439
InterPro:IPR011527
InterPro:IPR011917
InterPro:IPR017871
InterPro:IPR036640

F

Seeded From UniProt

complete

part_of

GO:0016021

integral component of membrane

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR011527
InterPro:IPR011917
InterPro:IPR036640

C

Seeded From UniProt

complete

enables

GO:0016887

ATPase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR003439
InterPro:IPR017871

F

Seeded From UniProt

complete

enables

GO:0034040

lipid-transporting ATPase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR011917

F

Seeded From UniProt

complete

enables

GO:0042626

ATPase activity, coupled to transmembrane movement of substances

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR011527

F

Seeded From UniProt

complete

involved_in

GO:0055085

transmembrane transport

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR011527

P

Seeded From UniProt

complete

enables

GO:0034040

lipid-transporting ATPase activity

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000100839

F

Seeded From UniProt

complete

part_of

GO:0005886

plasma membrane

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000100839

C

Seeded From UniProt

complete

involved_in

GO:0006869

lipid transport

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000100839

P

Seeded From UniProt

complete

involved_in

GO:0006869

lipid transport

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0445

P

Seeded From UniProt

complete

enables

GO:0005524

ATP binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0067

F

Seeded From UniProt

complete

part_of

GO:0005886

plasma membrane

GO_REF:0000037
GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0997
UniProtKB-KW:KW-1003
UniProtKB-SubCell:SL-0037

C

Seeded From UniProt

complete

part_of

GO:0016021

integral component of membrane

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0812

C

Seeded From UniProt

complete

part_of

GO:0016020

membrane

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0472

C

Seeded From UniProt

complete

enables

GO:0000166

nucleotide binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0547

F

Seeded From UniProt

complete

edit table

Notes

References

See Help:References for how to manage references in GONUTS.

  1. ↑ 1.0 1.1 Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed GONUTS page
  2. ↑ 2.0 2.1 2.2 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
  3. ↑ Moeller, A et al. (2015) Distinct conformational spectrum of homologous multidrug ABC transporters. Structure 23 450-460 PubMed GONUTS page
  4. ↑ 4.0 4.1 Ward, A et al. (2007) Flexibility in the ABC transporter MsbA: Alternating access with a twist. Proc. Natl. Acad. Sci. U.S.A. 104 19005-10 PubMed GONUTS page
  5. ↑ 5.0 5.1 5.2 Eckford, PD & Sharom, FJ (2008) Functional characterization of Escherichia coli MsbA: interaction with nucleotides and substrates. J. Biol. Chem. 283 12840-50 PubMed GONUTS page
  6. ↑ 6.0 6.1 6.2 6.3 6.4 Polissi, A & Georgopoulos, C (1996) Mutational analysis and properties of the msbA gene of Escherichia coli, coding for an essential ABC family transporter. Mol. Microbiol. 20 1221-33 PubMed GONUTS page
  7. ↑ 7.0 7.1 Eckford, PD & Sharom, FJ (2010) The reconstituted Escherichia coli MsbA protein displays lipid flippase activity. Biochem. J. 429 195-203 PubMed GONUTS page
  8. ↑ Doerrler, WT et al. (2004) MsbA-dependent translocation of lipids across the inner membrane of Escherichia coli. J. Biol. Chem. 279 45102-9 PubMed GONUTS page
  9. ↑ Doerrler, WT et al. (2001) An Escherichia coli mutant defective in lipid export. J. Biol. Chem. 276 11461-4 PubMed GONUTS page
  10. ↑ Doerrler, WT & Raetz, CR (2002) ATPase activity of the MsbA lipid flippase of Escherichia coli. J. Biol. Chem. 277 36697-705 PubMed GONUTS page
  11. ↑ Reuter, G et al. (2003) The ATP binding cassette multidrug transporter LmrA and lipid transporter MsbA have overlapping substrate specificities. J. Biol. Chem. 278 35193-8 PubMed GONUTS page
  12. ↑ Zhang, N et al. (2007) Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS. Proteomics 7 484-93 PubMed GONUTS page
  13. ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed GONUTS page
  14. ↑ Lopez-Campistrous, A et al. (2005) Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth. Mol. Cell Proteomics 4 1205-9 PubMed GONUTS page
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