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PMID:11278265

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Citation

Doerrler, WT, Reedy, MC and Raetz, CR (2001) An Escherichia coli mutant defective in lipid export. J. Biol. Chem. 276:11461-4

Abstract

Escherichia coli phospholipids and lipopolysaccharide, made on the inner surface of the inner membrane, are rapidly transported to the outer membrane by mechanisms that are not well characterized. We now report a temperature-sensitive mutant (WD2) with an A270T substitution in a trans-membrane region of the ABC transporter MsbA. As shown by (32)P(i) and (14)C-acetate labeling, export of all major lipids to the outer membrane is inhibited by approximately 90% in WD2 after 30 min at 44 degrees C. Transport of newly synthesized proteins is not impaired. Electron microscopy shows reduplicated inner membranes in WD2 at 44 degrees C, consistent with a key role for MsbA in lipid trafficking.

Links

PubMed Online version:10.1074/jbc.C100091200

Keywords

ATP-Binding Cassette Transporters; Bacterial Proteins/metabolism; Biological Transport; Chromatography, Thin Layer; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/ultrastructure; Lipid Metabolism; Membrane Proteins/metabolism; Microscopy, Electron; Point Mutation

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:MSBA

acts_upstream_of_or_within

GO:0015920: lipopolysaccharide transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:MSBA

involved_in

GO:0006869: lipid transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:MSBA

involved_in

GO:0015920: lipopolysaccharide transport

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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