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PMID:15256568

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Citation

Adelsberger, H, Hertel, C, Glawischnig, E, Zverlov, VV and Schwarz, WH (2004) Enzyme system of Clostridium stercorarium for hydrolysis of arabinoxylan: reconstitution of the in vivo system from recombinant enzymes. Microbiology (Reading, Engl.) 150:2257-66

Abstract

Four extracellular enzymes of the thermophilic bacterium Clostridium stercorarium are involved in the depolymerization of de-esterified arabinoxylan: Xyn11A, Xyn10C, Bxl3B, and Arf51B. They were identified in a collection of eight clones producing enzymes hydrolysing xylan (xynA, xynB, xynC), beta-xyloside (bxlA, bxlB, bglZ) and alpha-arabinofuranoside (arfA, arfB). The modular enzymes Xyn11A and Xyn10C represent the major xylanases in the culture supernatant of C. stercorarium. Both hydrolyse arabinoxylan in an endo-type mode, but differ in the pattern of the oligosaccharides produced. Of the glycosidases, Bxl3B degrades xylobiose and xylooligosaccharides to xylose, and Arf51B is able to release arabinose residues from de-esterified arabinoxylan and from the oligosaccharides generated. The other glycosidases either did not attack or only marginally attacked these oligosaccharides. Significantly more xylanase and xylosidase activity was produced during growth on xylose and xylan. This is believed to be the first time that, in a single thermophilic micro-organism, the complete set of enzymes (as well as the respective genes) to completely hydrolyse de-esterified arabinoxylan to its monomeric sugar constituents, xylose and arabinose, has been identified and the enzymes produced in vivo. The active enzyme system was reconstituted in vitro from recombinant enzymes.

Links

PubMed Online version:10.1099/mic.0.27066-0

Keywords

Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Clostridium/enzymology; Clostridium/genetics; Clostridium/growth & development; Culture Media; Gene Expression Regulation, Bacterial; Glycoside Hydrolases/metabolism; Hydrolysis; Molecular Sequence Data; Polysaccharides/metabolism; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Substrate Specificity; Xylans/metabolism; Xylosidases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CLOSR:Q8GJ42

GO:0045493: xylan catabolic process

ECO:0000314:

P

Figure 3 shows the hydrolysis of xylobiose to xylose. Xylobiose is a Xylan degradation product that is further degraded to xylose.

complete
CACAO 4383


See also

References

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