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User:Pierluis

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CACAO Spring 2014

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableMESMA:YA2014-04-01 11:49:08 CDTGO:0001515 opioid peptide activity (F)PMID:3392217ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows that BmK-YA interacts with the three subunits of opioid receptors, with preference to the δ- unit, as seen in Figue 1B.

challenge
updatedbyinstructorDANRE:S4TLP42014-04-01 14:54:19 CDTGO:0001077 RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription (F)PMID:24155663ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6A shows that in mutants who express the Znf219-Like protein, activity at the promotor of the col2a1 gene is substantially increased when compared to the wild-type control.

challenge
updatedbyinstructorXENLA:R4JU552014-04-03 08:04:58 CDTGO:0051017 actin filament bundle assembly (P)PMID:23727888ECO:0000314 direct assay evidence used in manual assertion

Figure 2D shows F-actin interacting with NabKin, resulting in bundling of the F-actin. Compare this image to the control image and you will see that there is a substantially larger amount of bundling in the presence of NabKin

challenge
unacceptableARATH:Q8LE892014-04-05 18:33:42 CDTGO:0003712 transcription cofactor activity (F)PMID:24569844ECO:0000314 direct assay evidence used in manual assertion

Figure 1 illustrates that members of the VQ family, in this study particularly, VQ29, have transcriptional activity according to the results of a heterologous gene reporter system protocol.

challenge
unacceptableARATH:Q8LE892014-04-05 18:49:28 CDTGO:0009704 de-etiolation (P)PMID:24569844ECO:0000315 mutant phenotype evidence used in manual assertion

VQ29 is shown to be a repressor of de-etiolation in Figure 2. Mutants overexpressing VQ29 showed reduced sensitivity to far-red and low light conditions, showing that in these conditions, VQ29 may have been inhibiting de-etiolation processes. This observation is further solidified in Figure 2D.

challenge

acceptable:1
unacceptable:2
requires_changes:0
flagged:0

Annotations challenged by Pierluis

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorDakwalea,
MichSt14A 30
LOCMI:S4W2D1GO:0016246 - RNA interference (P)PMID:23792802ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2

fixed by Dakwalea
acceptableMichelel,
MichSt14A 16
PHATR:L8B080GO:0005886 - plasma membrane (C)PMID:23297242ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
acceptableHLDose,
Team DRPs
CAMJE:Q0P8Q2GO:0016966 - nitric oxide reductase activity (P)PMID:17241202ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 4
unacceptableSarah.budde,
Team A-Squared
HELPX:Q9JP55GO:0016337 - single organismal cell-cell adhesion (P)PMID:8516329ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2
unacceptableEmilyNorwig,
Team A-Squared
CAMJJ:PEB1CGO:0006810 - transport (P)PMID:8360165ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
unacceptableDjgulizia,
Team Texas Aggies
HUMAN:GOLP3GO:0051645 - Golgi localization (P)PMID:24485452ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1

0 annotations fixed by Pierluis

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